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Family Report for: NLS3-Tex30

NLS3-Tex30



Relationship
Family NLS3-Tex30
Block X
Comment
This entry consists of the KAT8 regulatory NSL complex subunit 3 (KANSL3 or NSL3, also known as testis development protein PRTD) and the testis-expressed sequence 30 proteins (TEX30). KAT8 regulatory NSL complex subunit 3 is part of the NSL complex that is involved in acetylation of nucleosomal histone H4 on several lysine residues and therefore may be involved in the regulation of transcription. Meunier et al identified KANSL3 as a microtubule minus-end-binding protein. In C. Elegans SUMV-2 interacts with SUMV-1, and they may function together with MYS-2 in a nematode KAT8/MOF-like complex to antagonise the activity of the synMuv (synthetic multivulva) genes. The function of the testis-expressed sequence 30 protein is not known. Wu et al. showed that KANSL3 is a subunit of a nonspecific lethal (NSL) histone acetyltransferase (HAT) complex that acetylates core histone H4. NSL3 and the O-linked N-acetylglucosamine (O-GlcNAc) transferase OGT1 are subunits of an NSL HAT complex that acetylates histone H4 residues lys5 (K5), K8, and K16. Knockdown of OGT1 or NSL3 reduced global H4K16 acetylation in a dose-dependent manner. Immunoprecipitation analysis in HEK293 and HeLa cells revealed that endogenous OGT1 and NSL3 interacted, and mutation analysis revealed that the N-terminal TPR repeat domain of OGT1 interacted with the abhydrolase domain of NSL3. In vitro, OGT1 catalyzed O-GlcNAc modification of NSL3 in the presence of donor UDP-GlcNAc. The O-GlcNAc modification stabilized NSL3, and stabilized NSL3 was required for H4K16 acetylation by the HAT NSL complex

Database
Sequences
Interpro
|
IPR026555 (KAT8 regulatory NSL complex subunit 3/Testis-expressed sequence 30 protein), IPR046879 (KANL3/Tex30, alpha/beta hydrolase-like domain)
PIRSF
|
Pdoc
|
PFam
|
Prints
|
Prosite
|
no EC number



Peptide in
|Fasta
Nucleotide in
|Fasta
Alignment with Multalin
|Text only/graphic display
Seed alignment with MAFFT
|No colour/coloured with Mview
Alignment with MAFFT
|No colour/coloured with Mview
Dendrogram
|Graphical display, obtained with the dnd file produced by Clustalw

References
1 more
    Title: O-Linked N-acetylglucosamine transferase 1 regulates global histone H4 acetylation via stabilization of the nonspecific lethal protein NSL3
    Wu D, Zhao L, Feng Z, Yu C, Ding J, Wang L, Wang F, Liu D, Zhu H and Jin J <4 more author(s)>
    Ref: Journal of Biological Chemistry, 292:10014, 2017 : PubMed

            

    Title: An epigenetic regulator emerges as microtubule minus-end binding and stabilizing factor in mitosis
    Meunier S, Shvedunova M, Van Nguyen N, Avila L, Vernos I, Akhtar A
    Ref: Nat Commun, 6:7889, 2015 : PubMed

            

    Title: Subunit composition and substrate specificity of a MOF-containing histone acetyltransferase distinct from the male-specific lethal (MSL) complex
    Cai Y, Jin J, Swanson SK, Cole MD, Choi SH, Florens L, Washburn MP, Conaway JW, Conaway RC
    Ref: Journal of Biological Chemistry, 285:4268, 2010 : PubMed

            

Other Papers


No structure scheme yet for this family

No Structure yet in this family

Genes Proteins in NLS3-Tex30 family (676)

Fragments of genes in NLS3-Tex30 family (58)

No Substrate

No Inhibitor



Send your questions or comments to :
Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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