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Mutation Report for: A328W_human-BCHE

A328W_human-BCHE
Gene_Locus|human-BCHE
Mode of mutation|Site directed mutagenesis
Amino Acid change|A328W
Torpedo number|330
Summary|
Comment|p.A328W Ala328Trp (p.A356W Ala356Trp in primary sequence with 28 amino-acids signal peptide) Cocaine hydrolysis; hydrolyzed cocaine 15-fold faster compared with wild-type BChE
Kinetic parameters|none


References:
    Title: Wild-type and A328W mutant human butyrylcholinesterase tetramers expressed in Chinese hamster ovary cells have a 16-hour half-life in the circulation and protect mice from cocaine toxicity
    Duysen EG, Bartels CF, Lockridge O
    Ref: Journal of Pharmacology & Experimental Therapeutics, 302:751, 2002 : PubMed

            




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Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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