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Mutation Report for: F295I/F338A_human-ACHE

F295I/F338A_human-ACHE
Gene_Locus|human-ACHE
Mode of mutation|Site directed mutagenesis
Amino Acid change|F295I/F338A
Torpedo number|331,288//331//288
Summary|
Comment|p.F295I/F338A Phe295Ile/Phe338Ala (p.F326I/F369A Phe326Ile/Phe369Ala in primary sequence with 31 amino-acids signal peptide) Acyl specificity;Acyl pocket 1130-fold decreased catalytic activity, orientation of His 447
Kinetic parameters|none


References:
    Title: Functional requirements for the optimal catalytic configuration of the AChE active center
    Shafferman A, Barak D, Kaplan D, Ordentlich A, Kronman C, Velan B
    Ref: Chemico-Biological Interactions, 157-158:123, 2005 : PubMed

            




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Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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