Paper Report for: Pieper_2002_J.Bacteriol_184_1466
Reference
Title: Monitoring key reactions in degradation of chloroaromatics by in situ (1)H nuclear magnetic resonance: solution structures of metabolites formed from cis-dienelactone Pieper DH, Pollmann K, Nikodem P, Gonzalez B, Wray V Ref: Journal of Bacteriology, 184:1466, 2002 : PubMed
A (1)H nuclear magnetic resonance ((1)H NMR) assay was used to study the enzymatic transformation of cis-dienelactone, a central intermediate in the degradation of chloroaromatics. It was shown that the product of the cis-dienelactone hydrolase reaction is maleylacetate, in which there is no evidence for the formation of 3-hydroxymuconate. Under acidic conditions, the product structure was 4-carboxymethyl-4-hydroxybut-2-en-4-olide. Maleylacetate was transformed by maleylacetate reductase into 3-oxoadipate, a reaction competing with spontaneous decarboxylation into cis-acetylacrylate. One-dimensional (1)H NMR in (1)H(2)O could thus be shown to be an excellent noninvasive tool for monitoring enzyme activities and assessing the solution structure of substrates and products.
Pieper DH, Pollmann K, Nikodem P, Gonzalez B, Wray V (2002) Monitoring key reactions in degradation of chloroaromatics by in situ (1)H nuclear magnetic resonance: solution structures of metabolites formed from cis-dienelactone Journal of Bacteriology184: 1466-70
Pieper DH, Pollmann K, Nikodem P, Gonzalez B, Wray V (2002) Journal of Bacteriology184: 1466-70