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Author Report for: Wei HL

Title: Crystal Structure of a Mycoestrogen-Detoxifying Lactonase from Rhinocladiella mackenziei: Molecular Insight into ZHD Substrate Selectivity
Zheng YY, Liu WT, Chen CC, Hu XY, Liu WD, Ko TP, Tang XK, Wei HL, Huang JW, Guo RT
Ref: ACS Catal, 8:4294, 2018 : PubMed
Abstract


ESTHER: Zheng_2018_ACS.Catal_8_4294
PubMedSearch: Zheng 2018 ACS.Catal 8 4294
Gene_locus related to this paper: 9euro-a0a0d2ilk1

Abstract

Development of potent biocatalysts for enzymatic detoxification of estrogenic mycotoxin zearalenone (ZEN) and its more toxic derivative alpha-zearalenol (alpha-ZOL) is of great interest. Here, we report the crystal structures of a ZEN-hydrolyzing enzyme from Rhinocladiella mackenziei (RmZHD), including substrate complexes. A molecular mechanism for the distinct activity of RmZHD in hydrolyzing the structurally similar ZEN and alpha-ZOL is then proposed. In addition, structure-based engineering to modify the substrate-binding pocket and improve the RmZHD activity toward alpha-ZOL is presented. These results expand our scope in understanding the catalytic mechanism of ZHD-family enzymes and are of vital importance in further industrial applications.



        

Title: Complete genome sequence of the bacterium Ketogulonicigenium vulgare Y25
Xiong XH, Han S, Wang JH, Jiang ZH, Chen W, Jia N, Wei HL, Cheng H, Yang YX and Zhang WC <7 more author(s)> Xiong XH, Han S, Wang JH, Jiang ZH, Chen W, Jia N, Wei HL, Cheng H, Yang YX, Zhu B, You S, He JY, Hou W, Chen MX, Yu CJ, Jiao YH, Zhang WC (- 7)
Ref: Journal of Bacteriology, 193:315, 2011 : PubMed
Abstract


ESTHER: Xiong_2011_J.Bacteriol_193_315
PubMedSearch: Xiong 2011 J.Bacteriol 193 315
PubMedID: 21037005
Gene_locus related to this paper: ketvy-e3eyt7, ketvy-e3f614, ketvy-e3f0d0, ketvy-e3f2e6, ketvy-e3f2e7, ketvy-e3f5f9, ketvy-e3f2c7

Abstract

Ketogulonicigenium vulgare is characterized by the efficient production of 2KGA from L-sorbose. Ketogulonicigenium vulgare Y25 is known as a 2-keto-L-gulonic acid-producing strain in the vitamin C industry. Here we report the finished, annotated genome sequence of Ketogulonicigenium vulgare Y25.