Barak D, Ordentlich A, Stein D, Yu QS, Greig NH, Shafferman A (2009) <br />Accommodation of physostigmine and its analogues by acetylcholinesterase is dominated by hydrophobic interactions<br /><i>Biochemical Journal</i> <b>417</b>: 213<br /><br />Shafferman A, Barak D, Stein D, Kronman C, Velan B, Greig NH, Ordentlich A (2008) <br />Flexibility versus rigidity of the functional architecture of AChE active center<br /><i>Chemico-Biological Interactions</i> <b>175</b>: 166<br /><br />Pittel Z, Barak D, Segall Y (2006) <br />Function-specific blockage of M(1) and M(3) muscarinic acetylcholine receptors by VX and echothiophate<br /><i>Brain Research</i> <b>1085</b>: 102<br /><br />Barak D, Ordentlich A, Kaplan D, Kronman C, Velan B, Shafferman A (2005) <br />Lessons from functional analysis of AChE covalent and noncovalent inhibitors for design of AD therapeutic agents<br /><i>Chemico-Biological Interactions</i> <b>157-158</b>: 219<br /><br />Ordentlich A, Barak D, Sod-Moriah G, Kaplan D, Mizrahi D, Segall Y, Kronman C, Karton Y, Lazar A, Marcus D, Velan B, Shafferman A (2005) <br />The role of AChE active site gorge in determining stereoselectivity of charged and noncharged VX enantiomers<br /><i>Chemico-Biological Interactions</i> <b>157-158</b>: 191<br /><br />Shafferman A, Barak D, Kaplan D, Ordentlich A, Kronman C, Velan B (2005) <br />Functional requirements for the optimal catalytic configuration of the AChE active center<br /><i>Chemico-Biological Interactions</i> <b>157-158</b>: 123<br /><br />Barak D, Ordentlich A, Kaplan D, Elhanani E, Segall Y, Barak R, Velan B, Shafferman A (2004) <br />Poster (23) Phosphorylation and aging of AChE - insights from mutagenesis, mass spectrometry and structural studies<br /><i>In: Cholinesterases in the Second Millennium: Biomolecular and Pathological Aspects</i> <b> (Inestrosa NC, Campos EO) P. Universidad Catolica de Chile-FONDAP Biomedicina</b>: 332<br /><br />Kaplan D, Barak D, Ordentlich A, Kronman C, Velan B, Shafferman A (2004) <br />Is aromaticity essential for trapping the catalytic histidine 447 in human acetylcholinesterase?<br /><i>Biochemistry</i> <b>43</b>: 3129<br /><br />Kaplan D, Ordentlich A, Barak D, Ariel N, Kronman C, Baruch V, Shafferman A (2004) <br />Attempts to engineer an enzyme mimic of butyrylcholinesterase by substitution of the six divergent aromatic amino acids in the active center of acetylcholinesterase.<br /><i>Cholinergic Mechanisms, CRC Press</i> <br /><br />Ordentlich A, Barak D, Ariel N, Kronman C, Kaplan D, Velan B, Shafferman A (2004) <br />Surprising findings from the functional analysis of human acetylcholinesterase adducts of Alzheimer?s disease drugs.<br /><i>Cholinergic Mechanisms, CRC Press</i> <br /><br />Ordentlich A, Kronman C, Barak D, Ariel N, Kaplan D, Velan B, Shafferman A (2004) <br />Poster (21) Analysis of acetylcholinesterase adducts of alzheimer's drugs<br /><i>In: Cholinesterases in the Second Millennium: Biomolecular and Pathological Aspects</i> <b> (Inestrosa NC, Campos EO) P. Universidad Catolica de Chile-FONDAP Biomedicina</b>: 332<br /><br />Ordentlich A, Barak D, Sod-Moriah G, Kaplan D, Mizrahi D, Segall Y, Kronman C, Karton Y, Lazar A, Marcus D, Velan B, Shafferman A (2004) <br />Stereoselectivity toward VX is determined by interactions with residues of the acyl pocket as well as of the peripheral anionic site of AChE<br /><i>Biochemistry</i> <b>43</b>: 11255<br /><br />Shafferman A, Barak D, Kaplan D, Ordentlich A, Ariel N, Velan B (2004) <br />The aromatic trapping of histidine 447 in catalysis of acetylcholinesterases<br /><i>In: Cholinesterases in the Second Millennium: Biomolecular and Pathological Aspects</i> <b> (Inestrosa NC, Campos EO) P. Universidad Catolica de Chile-FONDAP Biomedicina</b>: 181 <br /><br />Shafferman A, Barak D, Kaplan D, Ordentlich A, Ariel N, Velan B (2004) <br />Poster (14) The aromatic trapping of histidine 447 in catalysis of acetylcholinesterases<br /><i>In: Cholinesterases in the Second Millennium: Biomolecular and Pathological Aspects</i> <b> (Inestrosa NC, Campos EO) P. Universidad Catolica de Chile-FONDAP Biomedicina</b>: 327<br /><br />Barak D, Kaplan D, Ordentlich A, Ariel N, Velan B, Shafferman A (2002) <br />The aromatic trapping of the catalytic histidine is essential for efficient catalysis in acetylcholinesterase<br /><i>Biochemistry</i> <b>41</b>: 8245<br /><br />Kaplan D, Ordentlich A, Barak D, Ariel N, Kronman C, Velan B, Shafferman A (2001) <br />Does butyrylization of acetylcholinesterase through substitution of the six divergent aromatic amino acids in the active center gorge generate an enzyme mimic of butyrylcholinesterase?<br /><i>Biochemistry</i> <b>40</b>: 7433<br /><br />Barak D, Ordentlich A, Kaplan D, Barak R, Mizrahi D, Kronman C, Segall Y, Velan B, Shafferman A (2000) <br />Evidence for P-N bond scission in phosphoroamidate nerve agent adducts of human acetylcholinesterase<br /><i>Biochemistry</i> <b>39</b>: 1156<br /><br />Kryger G, Harel M, Giles K, Toker L, Velan B, Lazar A, Kronman C, Barak D, Ariel N, Shafferman A, Silman I, Sussman JL (2000) <br />Structures of recombinant native and E202Q mutant human acetylcholinesterase complexed with the snake-venom toxin fasciculin-II<br /><i>Acta Crystallographica D Biol Crystallogr</i> <b>56</b>: 1385<br /><br />Millard CB, Kryger G, Ordentlich A, Greenblatt HM, Harel M, Raves ML, Segall Y, Barak D, Shafferman A, Silman I, Sussman JL (1999) <br />Crystal structures of aged phosphonylated acetylcholinesterase: nerve agent reaction products at the atomic level<br /><i>Biochemistry</i> <b>38</b>: 7032<br /><br />Ordentlich A, Barak D, Kronman C, Benschop HP, De Jong LP, Ariel N, Barak R, Segall Y, Velan B, Shafferman A (1999) <br />Exploring the active center of human acetylcholinesterase with stereomers of an organophosphorus inhibitor with two chiral centers<br /><i>Biochemistry</i> <b>38</b>: 3055<br /><br />Silman I, Millard CB, Ordentlich A, Greenblatt HM, Harel M, Barak D, Shafferman A, Sussman JL (1999) <br />A preliminary comparison of structural models for catalytic intermediates of acetylcholinesterase<br /><i>Chemico-Biological Interactions</i> <b>119-120</b>: 43<br /><br />Ariel N, Ordentlich A, Barak D, Bino T, Velan B, Shafferman A (1998) <br />Compatibility of Structures Inferred from Mutagenesis and from X-Ray Crystallography for Various AChE Complexes<br /><i>In: Structure and Function of Cholinesterases and Related Proteins - Proceedings of Sixth International Meeting on Cholinesterases</i> <b>(Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. Eds) Plenum Publishing Corp.</b>: 375<br /><br />Ariel N, Velan B, Barak D, Leitner M, Bino T, Ordentlich A, Shafferman A (1998) <br />Structural Modifications of the Omega Loop in Human Acetylcholinesterase<br /><i>In: Structure and Function of Cholinesterases and Related Proteins - Proceedings of Sixth International Meeting on Cholinesterases</i> <b>(Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. Eds) Plenum Publishing Corp.</b>: 453<br /><br />Ariel N, Ordentlich A, Barak D, Bino T, Velan B, Shafferman A (1998) <br />The 'aromatic patch' of three proximal residues in the human acetylcholinesterase active centre allows for versatile interaction modes with inhibitors<br /><i>Biochemical Journal</i> <b>335</b>: 95<br /><br />Barak D, Ordentlich A, Stein D, Segall Y, Velan B, Benschop HP, De Jong LP, Shafferman A (1998) <br />The Aromatic Moiety at Position-86 of HuAChE Accelerate the Aging of Phosphonyl-AChE Conjugates through Cation- Interactions<br /><i>In: Structure and Function of Cholinesterases and Related Proteins - Proceedings of Sixth International Meeting on Cholinesterases</i> <b>(Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. Eds) Plenum Publishing Corp.</b>: 246<br /><br />Kryger G, Giles K, Harel M, Toker L, Velan B, Lazar A, Kronman C, Barak D, Ariel N, Shafferman A, Silman I, Sussman JL (1998) <br />3D Structure at 2.7  Resolution of Native and E202Q Mutant Human Acetylcholinesterase Complexed with Fasciculin-II<br /><i>In: Structure and Function of Cholinesterases and Related Proteins - Proceedings of Sixth International Meeting on Cholinesterases</i> <b>(Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. Eds) Plenum Publishing Corp.</b>: 323<br /><br />Kryger G, Giles K, Harel M, Toker L, Velan B, Lazar A, Kronman C, Barak D, Ariel N, Shafferman A, Silman I, Sussman JL (1998) <br />3D Structure of a Complex of Human Acetylcholinesterase with Fasciculin-II at 2.7  Resolution<br /><i>In: Structure and Function of Cholinesterases and Related Proteins - Proceedings of Sixth International Meeting on Cholinesterases</i> <b>(Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. Eds) Plenum Publishing Corp.</b>: 370<br /><br />Millard CB, Kryger G, Ordentlich A, Harel M, Raves ML, Greenblatt HM, Segall Y, Barak D, Shafferman A, Silman I, Sussman JL (1998) <br />Crystal structure of Aged phosphorylated and phosphonylated Torpedo Californica Acetylcholinesterase<br /><i>In Structure and Function of Cholinesterases and Related Proteins - Proceedings of Sixth International Meeting on Cholinesterases</i> <b>(Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. Eds) Plenum Publishing Corp.</b>: 425<br /><br />Millard CB, Kryger G, Ordentlich A, Harel M, Raves ML, Greenblatt HM, Segall Y, Barak D, Shafferman A, Silman I, Sussman JL (1998) <br />Crystal Structures of Aged Phosphorylated and Phosphonylated Torpedo Californica Acetylcholinesterase<br /><i>In: Structure and Function of Cholinesterases and Related Proteins - Proceedings of Sixth International Meeting on Cholinesterases</i> <b>(Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. Eds) Plenum Publishing Corp.</b>: 425<br /><br />Millard CB, Kryger G, Ordentlich A, Harel M, Raves ML, Greenblatt HM, Segall Y, Barak D, Shafferman A, Silman I, Sussman JL (1998) <br />Crystal Structures of Aged Phosphorylated and Phosphonylated Torpedo Californica Acetylcholinesterase<br /><i>In: Structure and Function of Cholinesterases and Related Proteins - Proceedings of Sixth International Meeting on Cholinesterases</i> <b>(Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. Eds) Plenum Publishing Corp.</b>: 454<br /><br />Ordentlich A, Barak D, Stein D, Berman D, Kronman C, Ariel N, Velan B, Shafferman A (1998) <br />Does Electrostatic Attraction or Steering by Charged Residues within the Gorge Contribute to the Reactivity of AChE?<br /><i>In: Structure and Function of Cholinesterases and Related Proteins - Proceedings of Sixth International Meeting on Cholinesterases</i> <b>(Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. Eds) Plenum Publishing Corp.</b>: 234<br /><br />Ordentlich A, Barak R, Barak D, Fischer M, Benschop HP, De Jong LP, Segall Y, Velan B, Shafferman A (1998) <br />ESMS as a Unique Tool for the Molecular Monitoring of Reactions between HuAChE and Various OP-Agents<br /><i>In: Structure and Function of Cholinesterases and Related Proteins - Proceedings of Sixth International Meeting on Cholinesterases</i> <b>(Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. Eds) Plenum Publishing Corp.</b>: 249<br /><br />Ordentlich A, Barak D, Kronman C, Ariel N, Segall Y, Velan B, Shafferman A (1998) <br />Functional characteristics of the oxyanion hole in human acetylcholinesterase<br /><i>Journal of Biological Chemistry</i> <b>273</b>: 19509<br /><br />Shafferman A, Ordentlich A, Barak D, Kronman C, Ariel N, Velan B (1998) <br />Contribution of the Active Center Functional Architecture to AChE Reactivity Toward Substrates and Inhibitors<br /><i>In: Structure and Function of Cholinesterases and Related Proteins - Proceedings of Sixth International Meeting on Cholinesterases</i> <b>(Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. Eds) Plenum Publishing Corp.</b>: 203<br /><br />Barak R, Ordentlich A, Barak D, Fischer M, Benschop HP, De Jong LP, Segall Y, Velan B, Shafferman A (1997) <br />Direct determination of the chemical composition of acetylcholinesterase phosphonylation products utilizing electrospray- ionization mass spectrometry<br /><i>FEBS Letters</i> <b>407</b>: 347<br /><br />Shafferman A, Ordentlich A, Barak D, Stein D, Ariel N, Velan B (1997) <br />Aging of somanyl-acetylcholinesterase adducts: facts and models [letter]<br /><i>Biochemical Journal</i> <b>324</b>: 996<br /><br />Grosfeld H, Barak D, Ordentlich A, Velan B, Shafferman A (1996) <br />Interactions of oxime reactivators with diethylphosphoryl adducts of human acetylcholinesterase and its mutant derivatives<br /><i>Molecular Pharmacology</i> <b>50</b>: 639<br /><br />Ordentlich A, Barak D, Kronman C, Ariel N, Segall Y, Velan B, Shafferman A (1996) <br />The architecture of human acetylcholinesterase active center probed by interactions with selected organophosphate inhibitors<br /><i>Journal of Biological Chemistry</i> <b>271</b>: 11953<br /><br />Shafferman A, Ordentlich A, Barak D, Stein D, Ariel N, Velan B (1996) <br />Aging of phosphylated human acetylcholinesterase: catalytic processes mediated by aromatic and polar residues of the active centre<br /><i>Biochemical Journal</i> <b>318</b>: 833<br /><br />Velan B, Barak D, Ariel N, Leitner M, Bino T, Ordentlich A, Shafferman A (1996) <br />Structural modifications of the omega loop in human acetylcholinesterase<br /><i>FEBS Letters</i> <b>395</b>: 22<br /><br />Barak D, Ordentlich A, Kronman C, Ber R, Bino T, Ariel N, Osman R, Velan B, Shafferman A (1995) <br />Electrostatic Attraction by Surface Charge does not Contribute to the Catalytic Efficiency of Acetylcholinesterase<br /><i>In Enzyme of the Cholinesterase Family - Proceedings of Fifth International Meeting on Cholinesterases</i> <b>(Quinn, D.M., Balasubramanian, A.S., Doctor, B.P., Taylor, P., Eds) Plenum Publishing Corp.</b>: 223<br /><br />Barak D, Ordentlich A, Bromberg A, Kronman C, Marcus D, Lazar A, Ariel N, Velan B, Shafferman A (1995) <br />Allosteric modulation of acetylcholinesterase activity by peripheral ligands involves a conformational transition of the anionic subsite<br /><i>Biochemistry</i> <b>34</b>: 15444<br /><br />Ordentlich A, Kronman C, Stein D, Ariel N, Reuveny S, Marcus D, Segall Y, Barak D, Velan B, Shafferman A (1995) <br />Amino Acids Determining Specificity to OP-Agents and Facilitating the Aging Process in Human Acetylcholinesterase<br /><i>In Enzyme of the Cholinesterase Family - Proceedings of Fifth International Meeting on Cholinesterases</i> <b>(Quinn, D.M., Balasubramanian, A.S., Doctor, B.P., Taylor, P., Eds) Plenum Publishing Corp.</b>: 221<br /><br />Ordentlich A, Barak D, Kronman C, Ariel N, Segall Y, Velan B, Shafferman A (1995) <br />Contribution of aromatic moieties of tyrosine 133 and of the anionic subsite tryptophan 86 to catalytic efficiency and allosteric modulation of acetylcholinesterase<br /><i>Journal of Biological Chemistry</i> <b>270</b>: 2082<br /><br />Shafferman A, Ordentlich A, Barak D, Kronman C, Ariel N, Leitner M, Segall Y, Bromberg A, Reuveny S, Marcus D, Bino T, Lazar A, Cohen S, Velan B (1995) <br />Molecular Aspects of Catalysis and of Allosteric Regulation of Aceytlcholinesterases<br /><i>In Enzyme of the Cholinesterase Family - Proceedings of Fifth International Meeting on Cholinesterases</i> <b>(Quinn, D.M., Balasubramanian, A.S., Doctor, B.P., Taylor, P., Eds) Plenum Publishing Corp.</b>: 189<br /><br />Barak D, Kronman C, Ordentlich A, Ariel N, Bromberg A, Marcus D, Lazar A, Velan B, Shafferman A (1994) <br />Acetylcholinesterase peripheral anionic site degeneracy conferred by amino acid arrays sharing a common core<br /><i>Journal of Biological Chemistry</i> <b>269</b>: 6296<br /><br />Kronman C, Ordentlich A, Barak D, Velan B, Shafferman A (1994) <br />The back door hypothesis for product clearance in acetylcholinesterase challenged by site-directed mutagenesis<br /><i>Journal of Biological Chemistry</i> <b>269</b>: 27819<br /><br />Shafferman A, Ordentlich A, Barak D, Kronman C, Ber R, Bino T, Ariel N, Osman R, Velan B (1994) <br />Electrostatic attraction by surface charge does not contribute to the catalytic efficiency of acetylcholinesterase<br /><i>EMBO Journal</i> <b>13</b>: 3448<br /><br />Fisher A, Heldman E, Gurwitz D, Haring R, Barak D, Meshulam H, Marciano D, Brandeis R, Pittel Z, Segal M, et al. (1993) <br />Selective signaling via unique M1 muscarinic agonists<br /><i>Annals of the New York Academy of Sciences</i> <b>695</b>: 300<br /><br />Ordentlich A, Barak D, Kronman C, Flashner Y, Leitner M, Segall Y, Ariel N, Cohen S, Velan B, Shafferman A (1993) <br />Dissection of the human acetylcholinesterase active center determinants of substrate specificity. Identification of residues constituting the anionic site, the hydrophobic site, and the acyl pocket<br /><i>Journal of Biological Chemistry</i> <b>268</b>: 17083<br /><br />Ordentlich A, Kronman C, Barak D, Stein D, Ariel N, Marcus D, Velan B, Shafferman A (1993) <br />Engineering resistance to 'aging' of phosphylated human acetylcholinesterase. Role of hydrogen bond network in the active center<br /><i>FEBS Letters</i> <b>334</b>: 215<br /><br />Segall Y, Waysbort D, Barak D, Ariel N, Doctor BP, Grunwald J, Ashani Y (1993) <br />Direct observation and elucidation of the structures of aged and nonaged phosphorylated cholinesterases by 31P NMR spectroscopy<br /><i>Biochemistry</i> <b>32</b>: 13441<br /><br />Shafferman A, Velan B, Barak D, Kronman C, Ordentlich A, Flashner Y, Leitner M, Segal Y, Grosfeld H, Stein D, Ariel N (1993) <br />Recombinant human acetylcholinesterase - Enzyme engineering<br /><i>Medical Defense Bioscience Review</i> <b>3</b>: 1097<br /><br />Barak D, Ariel N, Velan B (1992) <br />Molecular Models for Human AChE and its Phosphonylation Products<br /><i>In Multidisciplinary approaches to cholinesterase functions - Proceedings of Fourth International Meeting on Cholinesterases</i> <b>(Shafferman, A. and Velan, B., Eds) Plenum Press, New York</b>: 195<br /><br />Shafferman A, Kronman C, Flashner Y, Leitner M, Grosfeld H, Ordentlich A, Gozes Y, Cohen S, Ariel N, Barak D, Harel M, Silman I, Sussman JL, Velan B (1992) <br />Mutagenesis of human acetylcholinesterase. Identification of residues involved in catalytic activity and in polypeptide folding<br /><i>Journal of Biological Chemistry</i> <b>267</b>: 17640<br /><br />Shafferman A, Velan B, Ordentlich A, Kronman C, Grosfeld H, Leitner M, Flashner Y, Cohen S, Barak D, Ariel N (1992) <br />Substrate inhibition of acetylcholinesterase: residues affecting signal transduction from the surface to the catalytic center<br /><i>EMBO Journal</i> <b>11</b>: 3561<br /><br />