Guimaraes JR, Carballares D, Tardioli PW, Rocha-Martin J, Fernandez-Lafuente R (2022)
Tuning Immobilized Commercial Lipase Preparations Features by Simple Treatment with Metallic Phosphate Salts
Molecules 27:

Guimaraes JR, Carballares D, Rocha-Martin J, Tardioli PW, Fernandez-Lafuente R (2022)
Tuning Immobilized Enzyme Features by Combining Solid-Phase Physicochemical Modification and Mineralization
Int J Mol Sci 23:

Souza PMP, Carballares D, Goncalves LRB, Fernandez-Lafuente R, Rodrigues S (2022)
Immobilization of Lipase B from Candida antarctica in Octyl-Vinyl Sulfone Agarose: Effect of the Enzyme-Support Interactions on Enzyme Activity, Specificity, Structure and Inactivation Pathway
Int J Mol Sci 23:

de Araujo-Silva R, Vieira AC, de Campos Giordano R, Fernandez-Lafuente R, Tardioli PW, de Torre MP, Cavero RY, Calvo MI (2022)
Anticholinesterase Activity of Selected Medicinal Plants from Navarra Region of Spain and a Detailed Phytochemical Investigation of Origanum vulgare L. ssp. vulgare
Molecules 27:

Monteiro RRC, Virgen-Ortiz JJ, Berenguer-Murcia A, da Rocha TN, Dos Santos JCS, Alcantara AR, Fernandez-Lafuente R (2021)
Biotechnological relevance of the lipase A from Candida antarctica
Catalysis Today 362: 141

Verdasco-Martin CM, Garcia-Verdugo E, Porcar R, Fernandez-Lafuente R, Otero C (2018)
Selective synthesis of partial glycerides of conjugated linoleic acids via modulation of the catalytic properties of lipases by immobilization on different supports
Food Chem 245: 39

Paludo N, Alves JS, Altmann C, Ayub MA, Fernandez-Lafuente R, Rodrigues RC (2015)
The combined use of ultrasound and molecular sieves improves the synthesis of ethyl butyrate catalyzed by immobilized Thermomyces lanuginosus lipase
Ultrason Sonochem 22: 89

Alves JS, Garcia-Galan C, Schein MF, Silva AM, Barbosa O, Ayub MA, Fernandez-Lafuente R, Rodrigues RC (2014)
Combined effects of ultrasound and immobilization protocol on butyl acetate synthesis catalyzed by CALB
Molecules 19: 9562

Martins AB, Friedrich JL, Cavalheiro JC, Garcia-Galan C, Barbosa O, Ayub MA, Fernandez-Lafuente R, Rodrigues RC (2013)
Improved production of butyl butyrate with lipase from Thermomyces lanuginosus immobilized on styrene-divinylbenzene beads
Bioresour Technol 134: 417

Martins AB, Schein MF, Friedrich JL, Fernandez-Lafuente R, Ayub MA, Rodrigues RC (2013)
Ultrasound-assisted butyl acetate synthesis catalyzed by Novozym 435: enhanced activity and operational stability
Ultrason Sonochem 20: 1155

Martins AB, Friedrich JL, Rodrigues RC, Garcia-Galan C, Fernandez-Lafuente R, Ayub MA (2013)
Optimized butyl butyrate synthesis catalyzed by Thermomyces lanuginosus lipase
Biotechnol Prog 29: 1416

Graebin NG, Martins AB, Lorenzoni AS, Garcia-Galan C, Fernandez-Lafuente R, Ayub MA, Rodrigues RC (2012)
Immobilization of lipase B from Candida antarctica on porous styrene-divinylbenzene beads improves butyl acetate synthesis
Biotechnol Prog 28: 406

Volpato G, Filice M, de Las Rivas B, Rodrigues RC, Heck JX, Fernandez-Lafuente R, Guisan JM, Mateo C, Ayub MA (2011)
Purification, immobilization, and characterization of a specific lipase from Staphylococcus warneri EX17 by enzyme fractionating via adsorption on different hydrophobic supports
Biotechnol Prog 27: 717

Carrasco-Lopez C, Godoy C, de Las Rivas B, Fernandez-Lorente G, Palomo JM, Guisan JM, Fernandez-Lafuente R, Martinez-Ripoll M, Hermoso JA (2009)
Activation of bacterial thermoalkalophilic lipases is spurred by dramatic structural rearrangements
Journal of Biological Chemistry 284: 4365

Cunha AG, Fernandez-Lorente G, Gutarra ML, Bevilaqua JV, Almeida RV, Paiva LM, Fernandez-Lafuente R, Guisan JM, Freire DM (2009)
Separation and immobilization of lipase from Penicillium simplicissimum by selective adsorption on hydrophobic supports
Appl Biochem Biotechnol 156: 133

Rodrigues RC, Bolivar JM, Volpato G, Filice M, Godoy C, Fernandez-Lafuente R, Guisan JM (2009)
Improved reactivation of immobilized-stabilized lipase from Thermomyces lanuginosus by its coating with highly hydrophilic polymers
J Biotechnol 144: 113

Carrasco-Lopez C, Godoy C, de Las Rivas B, Fernandez-Lorente G, Palomo JM, Guisan JM, Fernandez-Lafuente R, Martinez-Ripoll M, Hermoso JA (2008)
Crystallization and preliminary X-ray diffraction studies of the BTL2 lipase from the extremophilic microorganism Bacillus thermocatenulatus
Acta Crystallographica Sect F Struct Biol Cryst Commun 64: 1043

Cunha AG, Fernandez-Lorente G, Bevilaqua JV, Destain J, Paiva LM, Freire DM, Fernandez-Lafuente R, Guisan JM (2008)
Immobilization of Yarrowia lipolytica lipase--a comparison of stability of physical adsorption and covalent attachment techniques
Appl Biochem Biotechnol 146: 49

Fernandez-Lorente G, Godoy CA, Mendes AA, Lopez-Gallego F, Grazu V, de Las Rivas B, Palomo JM, Hermoso J, Fernandez-Lafuente R, Guisan JM (2008)
Solid-phase chemical amination of a lipase from Bacillus thermocatenulatus to improve its stabilization via covalent immobilization on highly activated glyoxyl-agarose
Biomacromolecules 9: 2553

Palomo JM, Segura RL, Fernandez-Lorente G, Pernas M, Rua ML, Guisan JM, Fernandez-Lafuente R (2004)
Purification, immobilization, and stabilization of a lipase from Bacillus thermocatenulatus by interfacial adsorption on hydrophobic supports
Biotechnol Prog 20: 630

Palomo JM, Ortiz C, Fuentes M, Fernandez-Lorente G, Guisan JM, Fernandez-Lafuente R (2004)
Use of immobilized lipases for lipase purification via specific lipase-lipase interactions
Journal of Chromatography A 1038: 267

Segura RL, Palomo JM, Mateo C, Cortes A, Terreni M, Fernandez-Lafuente R, Guisan JM (2004)
Different properties of the lipases contained in porcine pancreatic lipase extracts as enantioselective biocatalysts
Biotechnol Prog 20: 825

Mateo C, Archelas A, Fernandez-Lafuente R, Guisan JM, Furstoss R (2003)
Enzymatic transformations. Immobilized A. niger epoxide hydrolase as a novel biocatalytic tool for repeated-batch hydrolytic kinetic resolution of epoxides
Org Biomol Chem 1: 2739

Palomo JM, Penas MM, Fernandez-Lorente G, Mateo C, Pisabarro AG, Fernandez-Lafuente R, Ramirez L, Guisan JM (2003)
Solid-phase handling of hydrophobins: immobilized hydrophobins as a new tool to study lipases
Biomacromolecules 4: 204

Terreni M, Salvetti R, Linati L, Fernandez-Lafuente R, Fernandez-Lorente G, Bastida A, Guisan JM (2002)
Regioselective enzymatic hydrolysis of acetylated pyranoses and pyranosides using immobilised lipases. An easy chemoenzymatic synthesis of alpha- and beta-D-glucopyranose acetates bearing a free secondary C-4 hydroxyl group
Carbohydr Res 337: 1615

Bastida A, Fernandez-Lafuente R, Fernandez-Lorente G, Guisan JM, Pagani G, Terreni M (1999)
Regioselective hydrolysis of peracetylated alpha-D-glycopyranose catalyzed by immobilized lipases in aqueous medium. A facile preparation of useful intermediates for oligosaccharide synthesis
Bioorganic & Medicinal Chemistry Lett 9: 633

Wood AN, Fernandez-Lafuente R, Cowan DA (1995)
Purification and partial characterization of a novel thermophilic carboxylesterase with high mesophilic specific activity
Enzyme Microb Technol 17: 816