Distaso M, Cea-Rama I, Coscolin C, Chernikova TN, Tran H, Ferrer M, Sanz-Aparicio J, Golyshin PN (2023)
The Mobility of the Cap Domain Is Essential for the Substrate Promiscuity of a Family IV Esterase from Sorghum Rhizosphere Microbiome
Applied Environmental Microbiology

Molina-Espeja P, Fernandez-Lopez L, Golyshin PN, Ferrer M (2023)
Assigning Functions of Unknown Enzymes by High-Throughput Enzyme Characterization
Methods Mol Biol 2555: 181

Cea-Rama I, Coscolin C, Gonzalez-Alfonso JL, Raj J, Vasiljevic M, Plou FJ, Ferrer M, Sanz-Aparicio J (2022)
Crystal structure of a family VIII beta-lactamase fold hydrolase reveals the molecular mechanism for its broad substrate scope
Febs J

Vidal P, Martinez-Martinez M, Fernandez-Lopez L, Roda S, Mendez-Garcia C, Golyshina OV, Guallar V, Pelaez AI, Ferrer M (2022)
Metagenomic Mining for Esterases in the Microbial Community of Los Rueldos Acid Mine Drainage Formation
Front Microbiol 13: 868839

Xiang R, Fernandez-Lopez L, Robles-Martin A, Ferrer M, Guallar V (2022)
EP-Pred: A Machine Learning Tool for Bioprospecting Promiscuous Ester Hydrolases
Biomolecules 12:

Cea-Rama I, Coscolin C, Katsonis P, Bargiela R, Golyshin PN, Lichtarge O, Ferrer M, Sanz-Aparicio J (2021)
Structure and evolutionary trace-assisted screening of a residue swapping the substrate ambiguity and chiral specificity in an esterase
Comput Struct Biotechnol J 19: 2307

Hoppner A, Bollinger A, Kobus S, Thies S, Coscolin C, Ferrer M, Jaeger KE, Smits SHJ (2021)
Crystal structures of a novel family IV esterase in free and substrate-bound form
Febs J 288: 3570

Nutschel C, Coscolin C, David B, Mulnaes D, Ferrer M, Jaeger KE, Gohlke H (2021)
Promiscuous Esterases Counterintuitively Are Less Flexible than Specific Ones
J Chem Inf Model

Roda S, Fernandez-Lopez L, Canadas R, Santiago G, Ferrer M, Guallar V (2021)
Computationally Driven Rational Design of Substrate Promiscuity on Serine Ester Hydrolases
ACS Catal 11: 3590

Alonso S, Santiago G, Cea-Rama I, Fernandez-Lopez L, Coscolin C, Modregger J, Ressmann A, Martinez-Martinez M, Marrero H, Bargiela R, Pita M, Gonzalez-Alfonso JL, Briand M, Rojo D, Barbas C, Plou FJ, Golyshin PN, Shahgaldian P, Sanz-Aparicio J, Guallar V, Ferrer M (2019)
Genetically engineered proteins with two active sites for enhanced biocatalysis and synergistic chemo- and biocatalysis
Nature Catalysis 3: 319

Bollinger A, Thies S, Knieps-Grunhagen E, Gertzen C, Kobus S, Hoppner A, Ferrer M, Gohlke H, Smits SHJ, Jaeger KE (2020)
A Novel Polyester Hydrolase From the Marine Bacterium Pseudomonas aestusnigri - Structural and Functional Insights
Front Microbiol 11: 114

Bollinger A, Molitor R, Thies S, Koch R, Coscolin C, Ferrer M, Jaeger KE (2020)
Organic-Solvent-Tolerant Carboxylic Ester Hydrolases for Organic Synthesis
Applied Environmental Microbiology 86: e00106

Giunta CI, Cea-Rama I, Alonso S, Briand ML, Bargiela R, Coscolin C, Corvini PF, Ferrer M, Sanz-Aparicio J, Shahgaldian P (2020)
Tuning the Properties of Natural Promiscuous Enzymes by Engineering Their Nano-environment
ACS Nano

Chow J, Danso D, Ferrer M, Streit WR (2018)
The Thaumarchaeon N. gargensis carries functional bioABD genes and has a promiscuous E. coli DeltabioH-complementing esterase EstN1
Sci Rep 8: 13823

Martinez-Martinez M, Coscolin C, Santiago G, Chow J, Stogios PJ, Bargiela R, Gertler C, Navarro-Fernandez J, Bollinger A, Thies S, Mendez-Garcia C, Popovic A, Brown G, Chernikova TN, Garcia-Moyano A, Bjergah GE, Perez-Garcia P, Hai T, Del Pozo MV, Stokke R, Steen IH, Cui H, Xu X, Nocek BP, Alcaide M, Distaso M, Mesa V, Pelaez AI, Sanchez J, Buchholz PCF, Pleiss J, Fernandez-Guerra A, Glockner FO, Golyshina OV, Yakimov MM, Savchenko A, Jaeger KE, Yakunin AF, Streit WR, Golyshin PN, Guallar V, Ferrer M (2018)
Determinants and prediction of esterase substrate promiscuity patterns
ACS Chemical Biology 13: 225

Reyes-Duarte D, Coscolin C, Martinez-Martinez M, Ferrer M, Garcia-Arellano H (2018)
Functional-Based Screening Methods for Detecting Esterase and Lipase Activity Against Multiple Substrates
Methods Mol Biol 1835: 109

Santiago G, Martinez-Martinez M, Alonso S, Bargiela R, Coscolin C, Golyshin PN, Guallar V, Ferrer M (2018)
Rational Engineering of Multiple Active Sites in an Ester Hydrolase
Biochemistry 57: 2245

Popovic A, Hai T, Tchigvintsev A, Hajighasemi M, Nocek B, Khusnutdinova AN, Brown G, Glinos J, Flick R, Skarina T, Chernikova TN, Yim V, Bruls T, Paslier DL, Yakimov MM, Joachimiak A, Ferrer M, Golyshina OV, Savchenko A, Golyshin PN, Yakunin AF (2017)
Activity screening of environmental metagenomic libraries reveals novel carboxylesterase families
Sci Rep 7: 44103

Pena-Garcia C, Martinez-Martinez M, Reyes-Duarte D, Ferrer M (2016)
High Throughput Screening of Esterases, Lipases and Phospholipases in Mutant and Metagenomic Libraries: A Review
Comb Chem High Throughput Screen 19: 605

Alcaide M, Stogios PJ, Lafraya A, Tchigvintsev A, Flick R, Bargiela R, Chernikova TN, Reva ON, Hai T, Leggewie CC, Katzke N, La Cono V, Matesanz R, Jebbar M, Jaeger KE, Yakimov MM, Yakunin AF, Golyshin PN, Golyshina OV, Savchenko A, Ferrer M (2015)
Pressure adaptation is linked to thermal adaptation in salt-saturated marine habitats
Environ Microbiol 17: 332

Placido A, Hai T, Ferrer M, Chernikova TN, Distaso M, Armstrong D, Yakunin AF, Toshchakov SV, Yakimov MM, Kublanov IV, Golyshina OV, Pesole G, Ceci LR, Golyshin PN (2015)
Diversity of hydrolases from hydrothermal vent sediments of the Levante Bay, Vulcano Island (Aeolian archipelago) identified by activity-based metagenomics and biochemical characterization of new esterases and an arabinopyranosidase
Applied Microbiology & Biotechnology 99: 10031

Martinez-Martinez M, Lores I, Pena-Garcia C, Bargiela R, Reyes-Duarte D, Guazzaroni ME, Pelaez AI, Sanchez J, Ferrer M (2014)
Biochemical studies on a versatile esterase that is most catalytically active with polyaromatic esters
Microb Biotechnol

Mendez-Garcia C, Mesa V, Sprenger RR, Richter M, Diez MS, Solano J, Bargiela R, Golyshina OV, Manteca A, Ramos JL, Gallego JR, Llorente I, Martins dos Santos VA, Jensen ON, Pelaez AI, Sanchez J, Ferrer M (2014)
Microbial stratification in low pH oxic and suboxic macroscopic growths along an acid mine drainage
Isme J 8: 1259

Alcaide M, Tornes J, Stogios PJ, Xu X, Gertler C, Di Leo R, Bargiela R, Lafraya A, Guazzaroni ME, Lopez-Cortes N, Chernikova TN, Golyshina OV, Nechitaylo TY, Plumeier I, Pieper DH, Yakimov MM, Savchenko A, Golyshin PN, Ferrer M (2013)
Single residues dictate the co-evolution of dual esterases: MCP hydrolases from the alpha/beta hydrolase family
Biochemical Journal 454: 157

Ferrer M, Ruiz A, Lanza F, Haange SB, Oberbach A, Till H, Bargiela R, Campoy C, Segura MT, Richter M, von Bergen M, Seifert J, Suarez A (2013)
Microbiota from the distal guts of lean and obese adolescents exhibit partial functional redundancy besides clear differences in community structure
Environ Microbiol 15: 211

Golyshin PN, Werner J, Chernikova TN, Tran H, Ferrer M, Yakimov MM, Teeling H, Golyshina OV (2013)
Genome Sequence of Thalassolituus oleivorans MIL-1 (DSM 14913T)
Genome Announc 1: e0014113

Kube M, Chernikova TN, Al-Ramahi Y, Beloqui A, Lopez-Cortez N, Guazzaroni ME, Heipieper HJ, Klages S, Kotsyurbenko OR, Langer I, Nechitaylo TY, Lunsdorf H, Fernandez M, Juarez S, Ciordia S, Singer A, Kagan O, Egorova O, Petit PA, Stogios P, Kim Y, Tchigvintsev A, Flick R, Denaro R, Genovese M, Albar JP, Reva ON, Martinez-Gomariz M, Tran H, Ferrer M, Savchenko A, Yakunin AF, Yakimov MM, Golyshina OV, Reinhardt R, Golyshin PN (2013)
Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica
Nat Commun 4: 2156

Martinez-Martinez M, Alcaide M, Tchigvintsev A, Reva O, Polaina J, Bargiela R, Guazzaroni ME, Chicote A, Canet A, Valero F, Rico Eguizabal E, Guerrero Mdel C, Yakunin AF, Ferrer M (2013)
Biochemical diversity of carboxyl esterases and lipases from lake arreo (Spain): a metagenomic approach
Applied Environmental Microbiology 79: 3553

Alcaide M, Messina E, Richter M, Bargiela R, Peplies J, Huws SA, Newbold CJ, Golyshin PN, Simon MA, Lopez G, Yakimov MM, Ferrer M (2012)
Gene sets for utilization of primary and secondary nutrition supplies in the distal gut of endangered Iberian lynx
PLoS ONE 7: e51521

Del Pozo MV, Fernandez-Arrojo L, Gil-Martinez J, Montesinos A, Chernikova TN, Nechitaylo TY, Waliszek A, Tortajada M, Rojas A, Huws SA, Golyshina OV, Newbold CJ, Polaina J, Ferrer M, Golyshin PN (2012)
Microbial beta-glucosidases from cow rumen metagenome enhance the saccharification of lignocellulose in combination with commercial cellulase cocktail
Biotechnol Biofuels 5: 73

Reyes-Duarte D, Ferrer M, Garcia-Arellano H (2012)
Functional-based screening methods for lipases, esterases, and phospholipases in metagenomic libraries
Methods Mol Biol 861: 101

Ferrer M, Guazzaroni ME, Richter M, Garcia-Salamanca A, Yarza P, Suarez-Suarez A, Solano J, Alcaide M, van Dillewijn P, Molina-Henares MA, Lopez-Cortes N, Al-Ramahi Y, Guerrero C, Acosta A, de Eugenio LI, Martinez V, Marques S, Rojo F, Santero E, Genilloud O, Perez-Perez J, Rossello-Mora R, Ramos JL (2011)
Taxonomic and functional metagenomic profiling of the microbial community in the anoxic sediment of a sub-saline shallow lake (Laguna de Carrizo, Central Spain)
Microb Ecol 62: 824

Beloqui A, Polaina J, Vieites JM, Reyes-Duarte D, Torres R, Golyshina OV, Chernikova TN, Waliczek A, Aharoni A, Yakimov MM, Timmis KN, Golyshin PN, Ferrer M (2010)
Novel hybrid esterase-haloacid dehalogenase enzyme
Chembiochem 11: 1975

Beloqui A, Nechitaylo TY, Lopez-Cortes N, Ghazi A, Guazzaroni ME, Polaina J, Strittmatter AW, Reva O, Waliczek A, Yakimov MM, Golyshina OV, Ferrer M, Golyshin PN (2010)
Diversity of glycosyl hydrolases from cellulose-depleting communities enriched from casts of two earthworm species
Applied Environmental Microbiology 76: 5934

Vieites JM, Ghazi A, Beloqui A, Polaina J, Andreu JM, Golyshina OV, Nechitaylo TY, Waliczek A, Yakimov MM, Golyshin PN, Ferrer M (2010)
Inter-conversion of catalytic abilities in a bifunctional carboxyl/feruloyl-esterase from earthworm gut metagenome
Microb Biotechnol 3: 48

Golyshina OV, Golyshin PN, Timmis KN, Ferrer M (2006)
The 'pH optimum anomaly' of intracellular enzymes of Ferroplasma acidiphilum
Environ Microbiol 8: 416

Schneiker S, Martins dos Santos VA, Bartels D, Bekel T, Brecht M, Buhrmester J, Chernikova TN, Denaro R, Ferrer M, Gertler C, Goesmann A, Golyshina OV, Kaminski F, Khachane AN, Lang S, Linke B, McHardy AC, Meyer F, Nechitaylo T, Puhler A, Regenhardt D, Rupp O, Sabirova JS, Selbitschka W, Yakimov MM, Timmis KN, Vorholter FJ, Weidner S, Kaiser O, Golyshin PN (2006)
Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis
Nat Biotechnol 24: 997

Ferrer M, Golyshina OV, Chernikova TN, Khachane AN, Martins dos Santos VA, Yakimov MM, Timmis KN, Golyshin PN (2005)
Microbial enzymes mined from the Urania deep-sea hypersaline anoxic basin
Chemical Biology 12: 895

Ferrer M, Golyshina OV, Chernikova TN, Khachane AN, Reyes-Duarte D, Santos VA, Strompl C, Elborough K, Jarvis G, Neef A, Yakimov MM, Timmis KN, Golyshin PN (2005)
Novel hydrolase diversity retrieved from a metagenome library of bovine rumen microflora
Environ Microbiol 7: 1996

Reyes-Duarte D, Polaina J, Lopez-Cortes N, Alcalde M, Plou FJ, Elborough K, Ballesteros A, Timmis KN, Golyshin PN, Ferrer M (2005)
Conversion of a carboxylesterase into a triacylglycerol lipase by a random mutation
Angew Chem Int Ed Engl 44: 7553

Ferrer M, Chernikova TN, Timmis KN, Golyshin PN (2004)
Expression of a temperature-sensitive esterase in a novel chaperone-based Escherichia coli strain
Applied Environmental Microbiology 70: 4499