Distaso M, Cea-Rama I, Coscolin C, Chernikova TN, Tran H, Ferrer M, Sanz-Aparicio J, Golyshin PN (2023) <br />The Mobility of the Cap Domain Is Essential for the Substrate Promiscuity of a Family IV Esterase from Sorghum Rhizosphere Microbiome<br /><i>Applied Environmental Microbiology</i> <br /><br />Distaso MA, Chernikova TN, Bargiela R, Coscolin C, Stogios P, Gonzalez-Alfonso JL, Lemak S, Khusnutdinova AN, Plou FJ, Evdokimova E, Savchenko A, Lunev EA, Yakimov MM, Golyshina OV, Ferrer M, Yakunin AF, Golyshin PN (2023) <br />Thermophilic Carboxylesterases from Hydrothermal Vents of the Volcanic Island of Ischia Active on Synthetic and Biobased Polymers and Mycotoxins<br /><i>Applied Environmental Microbiology</i> <br /><br />Molina-Espeja P, Fernandez-Lopez L, Golyshin PN, Ferrer M (2023) <br />Assigning Functions of Unknown Enzymes by High-Throughput Enzyme Characterization<br /><i>Methods Mol Biol</i> <b>2555</b>: 181<br /><br />Williams GB, Ma H, Khusnutdinova AN, Yakunin AF, Golyshin PN (2023) <br />Harnessing extremophilic carboxylesterases for applications in polyester depolymerisation and plastic waste recycling<br /><i>Essays Biochem</i> <br /><br />Cea-Rama I, Coscolin C, Katsonis P, Bargiela R, Golyshin PN, Lichtarge O, Ferrer M, Sanz-Aparicio J (2021) <br />Structure and evolutionary trace-assisted screening of a residue swapping the substrate ambiguity and chiral specificity in an esterase<br /><i>Comput Struct Biotechnol J</i> <b>19</b>: 2307<br /><br />Alonso S, Santiago G, Cea-Rama I, Fernandez-Lopez L, Coscolin C, Modregger J, Ressmann A, Martinez-Martinez M, Marrero H, Bargiela R, Pita M, Gonzalez-Alfonso JL, Briand M, Rojo D, Barbas C, Plou FJ, Golyshin PN, Shahgaldian P, Sanz-Aparicio J, Guallar V, Ferrer M (2019) <br />Genetically engineered proteins with two active sites for enhanced biocatalysis and synergistic chemo- and biocatalysis<br /><i>Nature Catalysis</i> <b>3</b>: 319<br /><br />Hajighasemi M, Tchigvintsev A, Nocek B, Flick R, Popovic A, Hai T, Khusnutdinova AN, Brown G, Xu X, Cui H, Anstett J, Chernikova TN, Bruls T, Le Paslier D, Yakimov MM, Joachimiak A, Golyshina OV, Savchenko A, Golyshin PN, Edwards EA, Yakunin AF (2018) <br />Screening and Characterization of Novel Polyesterases from Environmental Metagenomes with High Hydrolytic Activity against Synthetic Polyesters<br /><i>Environ Sci Technol</i> <b>52</b>: 12388<br /><br />Martinez-Martinez M, Coscolin C, Santiago G, Chow J, Stogios PJ, Bargiela R, Gertler C, Navarro-Fernandez J, Bollinger A, Thies S, Mendez-Garcia C, Popovic A, Brown G, Chernikova TN, Garcia-Moyano A, Bjergah GE, Perez-Garcia P, Hai T, Del Pozo MV, Stokke R, Steen IH, Cui H, Xu X, Nocek BP, Alcaide M, Distaso M, Mesa V, Pelaez AI, Sanchez J, Buchholz PCF, Pleiss J, Fernandez-Guerra A, Glockner FO, Golyshina OV, Yakimov MM, Savchenko A, Jaeger KE, Yakunin AF, Streit WR, Golyshin PN, Guallar V, Ferrer M (2018) <br />Determinants and prediction of esterase substrate promiscuity patterns<br /><i>ACS Chemical Biology</i> <b>13</b>: 225<br /><br />Santiago G, Martinez-Martinez M, Alonso S, Bargiela R, Coscolin C, Golyshin PN, Guallar V, Ferrer M (2018) <br />Rational Engineering of Multiple Active Sites in an Ester Hydrolase<br /><i>Biochemistry</i> <b>57</b>: 2245<br /><br />Popovic A, Hai T, Tchigvintsev A, Hajighasemi M, Nocek B, Khusnutdinova AN, Brown G, Glinos J, Flick R, Skarina T, Chernikova TN, Yim V, Bruls T, Paslier DL, Yakimov MM, Joachimiak A, Ferrer M, Golyshina OV, Savchenko A, Golyshin PN, Yakunin AF (2017) <br />Activity screening of environmental metagenomic libraries reveals novel carboxylesterase families<br /><i>Sci Rep</i> <b>7</b>: 44103<br /><br />Hajighasemi M, Nocek BP, Tchigvintsev A, Brown G, Flick R, Xu X, Cui H, Hai T, Joachimiak A, Golyshin PN, Savchenko A, Edwards EA, Yakunin AF (2016) <br />Biochemical and Structural Insights into Enzymatic Depolymerization of Polylactic Acid and Other Polyesters by Microbial Carboxylesterases<br /><i>Biomacromolecules</i> <b>17</b>: 2027<br /><br />Alcaide M, Stogios PJ, Lafraya A, Tchigvintsev A, Flick R, Bargiela R, Chernikova TN, Reva ON, Hai T, Leggewie CC, Katzke N, La Cono V, Matesanz R, Jebbar M, Jaeger KE, Yakimov MM, Yakunin AF, Golyshin PN, Golyshina OV, Savchenko A, Ferrer M (2015) <br />Pressure adaptation is linked to thermal adaptation in salt-saturated marine habitats<br /><i>Environ Microbiol</i> <b>17</b>: 332<br /><br />Placido A, Hai T, Ferrer M, Chernikova TN, Distaso M, Armstrong D, Yakunin AF, Toshchakov SV, Yakimov MM, Kublanov IV, Golyshina OV, Pesole G, Ceci LR, Golyshin PN (2015) <br />Diversity of hydrolases from hydrothermal vent sediments of the Levante Bay, Vulcano Island (Aeolian archipelago) identified by activity-based metagenomics and biochemical characterization of new esterases and an arabinopyranosidase<br /><i>Applied Microbiology & Biotechnology</i> <b>99</b>: 10031<br /><br />Prive F, Newbold CJ, Kaderbhai NN, Girdwood SG, Golyshina OV, Golyshin PN, Scollan ND, Huws SA (2015) <br />Isolation and characterization of novel lipases/esterases from a bovine rumen metagenome<br /><i>Applied Microbiology & Biotechnology</i> <b>99</b>: 5475<br /><br />Tchigvintsev A, Tran H, Popovic A, Kovacic F, Brown G, Flick R, Hajighasemi M, Egorova O, Somody JC, Tchigvintsev D, Khusnutdinova A, Chernikova TN, Golyshina OV, Yakimov MM, Savchenko A, Golyshin PN, Jaeger KE, Yakunin AF (2015) <br />The environment shapes microbial enzymes: five cold-active and salt-resistant carboxylesterases from marine metagenomes<br /><i>Applied Microbiology & Biotechnology</i> <b>99</b>: 2165<br /><br />Alcaide M, Tornes J, Stogios PJ, Xu X, Gertler C, Di Leo R, Bargiela R, Lafraya A, Guazzaroni ME, Lopez-Cortes N, Chernikova TN, Golyshina OV, Nechitaylo TY, Plumeier I, Pieper DH, Yakimov MM, Savchenko A, Golyshin PN, Ferrer M (2013) <br />Single residues dictate the co-evolution of dual esterases: MCP hydrolases from the alpha/beta hydrolase family<br /><i>Biochemical Journal</i> <b>454</b>: 157<br /><br />Golyshin PN, Werner J, Chernikova TN, Tran H, Ferrer M, Yakimov MM, Teeling H, Golyshina OV (2013) <br />Genome Sequence of Thalassolituus oleivorans MIL-1 (DSM 14913T)<br /><i>Genome Announc</i> <b>1</b>: e0014113<br /><br />Kube M, Chernikova TN, Al-Ramahi Y, Beloqui A, Lopez-Cortez N, Guazzaroni ME, Heipieper HJ, Klages S, Kotsyurbenko OR, Langer I, Nechitaylo TY, Lunsdorf H, Fernandez M, Juarez S, Ciordia S, Singer A, Kagan O, Egorova O, Petit PA, Stogios P, Kim Y, Tchigvintsev A, Flick R, Denaro R, Genovese M, Albar JP, Reva ON, Martinez-Gomariz M, Tran H, Ferrer M, Savchenko A, Yakunin AF, Yakimov MM, Golyshina OV, Reinhardt R, Golyshin PN (2013) <br />Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica<br /><i>Nat Commun</i> <b>4</b>: 2156<br /><br />Alcaide M, Messina E, Richter M, Bargiela R, Peplies J, Huws SA, Newbold CJ, Golyshin PN, Simon MA, Lopez G, Yakimov MM, Ferrer M (2012) <br />Gene sets for utilization of primary and secondary nutrition supplies in the distal gut of endangered Iberian lynx<br /><i>PLoS ONE</i> <b>7</b>: e51521<br /><br />Del Pozo MV, Fernandez-Arrojo L, Gil-Martinez J, Montesinos A, Chernikova TN, Nechitaylo TY, Waliszek A, Tortajada M, Rojas A, Huws SA, Golyshina OV, Newbold CJ, Polaina J, Ferrer M, Golyshin PN (2012) <br />Microbial beta-glucosidases from cow rumen metagenome enhance the saccharification of lignocellulose in combination with commercial cellulase cocktail<br /><i>Biotechnol Biofuels</i> <b>5</b>: 73<br /><br />Lemak S, Tchigvintsev A, Petit P, Flick R, Singer AU, Brown G, Evdokimova E, Egorova O, Gonzalez CF, Chernikova TN, Yakimov MM, Kube M, Reinhardt R, Golyshin PN, Savchenko A, Yakunin AF (2012) <br />Structure and activity of the cold-active and anion-activated carboxyl esterase OLEI01171 from the oil-degrading marine bacterium Oleispira antarctica<br /><i>Biochemical Journal</i> <b>445</b>: 193<br /><br />Beloqui A, Polaina J, Vieites JM, Reyes-Duarte D, Torres R, Golyshina OV, Chernikova TN, Waliczek A, Aharoni A, Yakimov MM, Timmis KN, Golyshin PN, Ferrer M (2010) <br />Novel hybrid esterase-haloacid dehalogenase enzyme<br /><i>Chembiochem</i> <b>11</b>: 1975<br /><br />Beloqui A, Nechitaylo TY, Lopez-Cortes N, Ghazi A, Guazzaroni ME, Polaina J, Strittmatter AW, Reva O, Waliczek A, Yakimov MM, Golyshina OV, Ferrer M, Golyshin PN (2010) <br />Diversity of glycosyl hydrolases from cellulose-depleting communities enriched from casts of two earthworm species<br /><i>Applied Environmental Microbiology</i> <b>76</b>: 5934<br /><br />Vieites JM, Ghazi A, Beloqui A, Polaina J, Andreu JM, Golyshina OV, Nechitaylo TY, Waliczek A, Yakimov MM, Golyshin PN, Ferrer M (2010) <br />Inter-conversion of catalytic abilities in a bifunctional carboxyl/feruloyl-esterase from earthworm gut metagenome<br /><i>Microb Biotechnol</i> <b>3</b>: 48<br /><br />Golyshina OV, Golyshin PN, Timmis KN, Ferrer M (2006) <br />The 'pH optimum anomaly' of intracellular enzymes of Ferroplasma acidiphilum<br /><i>Environ Microbiol</i> <b>8</b>: 416<br /><br />Schneiker S, Martins dos Santos VA, Bartels D, Bekel T, Brecht M, Buhrmester J, Chernikova TN, Denaro R, Ferrer M, Gertler C, Goesmann A, Golyshina OV, Kaminski F, Khachane AN, Lang S, Linke B, McHardy AC, Meyer F, Nechitaylo T, Puhler A, Regenhardt D, Rupp O, Sabirova JS, Selbitschka W, Yakimov MM, Timmis KN, Vorholter FJ, Weidner S, Kaiser O, Golyshin PN (2006) <br />Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis<br /><i>Nat Biotechnol</i> <b>24</b>: 997<br /><br />Ferrer M, Golyshina OV, Chernikova TN, Khachane AN, Martins dos Santos VA, Yakimov MM, Timmis KN, Golyshin PN (2005) <br />Microbial enzymes mined from the Urania deep-sea hypersaline anoxic basin<br /><i>Chemical Biology</i> <b>12</b>: 895<br /><br />Ferrer M, Golyshina OV, Chernikova TN, Khachane AN, Reyes-Duarte D, Santos VA, Strompl C, Elborough K, Jarvis G, Neef A, Yakimov MM, Timmis KN, Golyshin PN (2005) <br />Novel hydrolase diversity retrieved from a metagenome library of bovine rumen microflora<br /><i>Environ Microbiol</i> <b>7</b>: 1996<br /><br />Reyes-Duarte D, Polaina J, Lopez-Cortes N, Alcalde M, Plou FJ, Elborough K, Ballesteros A, Timmis KN, Golyshin PN, Ferrer M (2005) <br />Conversion of a carboxylesterase into a triacylglycerol lipase by a random mutation<br /><i>Angew Chem Int Ed Engl</i> <b>44</b>: 7553<br /><br />Yakimov MM, Denaro R, Genovese M, Cappello S, D'Auria G, Chernikova TN, Timmis KN, Golyshin PN, Giluliano L (2005) <br />Natural microbial diversity in superficial sediments of Milazzo Harbor (Sicily) and community successions during microcosm enrichment with various hydrocarbons<br /><i>Environ Microbiol</i> <b>7</b>: 1426<br /><br />Ferrer M, Chernikova TN, Timmis KN, Golyshin PN (2004) <br />Expression of a temperature-sensitive esterase in a novel chaperone-based Escherichia coli strain<br /><i>Applied Environmental Microbiology</i> <b>70</b>: 4499<br /><br />Yakimov MM, Kroeger A, Slepak TN, Giuliano L, Timmis KN, Golyshin PN (1998) <br />A putative lichenysin A synthetase operon in B.licheniformis: initial charachterization.<br /><i>Biochimica & Biophysica Acta</i> <b>1399</b>: 141<br /><br />