Cea-Rama I, Coscolin C, Katsonis P, Bargiela R, Golyshin PN, Lichtarge O, Ferrer M, Sanz-Aparicio J (2021)
Structure and evolutionary trace-assisted screening of a residue swapping the substrate ambiguity and chiral specificity in an esterase
Comput Struct Biotechnol J 19: 2307

Alonso S, Santiago G, Cea-Rama I, Fernandez-Lopez L, Coscolin C, Modregger J, Ressmann A, Martinez-Martinez M, Marrero H, Bargiela R, Pita M, Gonzalez-Alfonso JL, Briand M, Rojo D, Barbas C, Plou FJ, Golyshin PN, Shahgaldian P, Sanz-Aparicio J, Guallar V, Ferrer M (2019)
Genetically engineered proteins with two active sites for enhanced biocatalysis and synergistic chemo- and biocatalysis
Nature Catalysis 3: 319

Hajighasemi M, Tchigvintsev A, Nocek B, Flick R, Popovic A, Hai T, Khusnutdinova AN, Brown G, Xu X, Cui H, Anstett J, Chernikova TN, Bruls T, Le Paslier D, Yakimov MM, Joachimiak A, Golyshina OV, Savchenko A, Golyshin PN, Edwards EA, Yakunin AF (2018)
Screening and Characterization of Novel Polyesterases from Environmental Metagenomes with High Hydrolytic Activity against Synthetic Polyesters
Environ Sci Technol 52: 12388

Martinez-Martinez M, Coscolin C, Santiago G, Chow J, Stogios PJ, Bargiela R, Gertler C, Navarro-Fernandez J, Bollinger A, Thies S, Mendez-Garcia C, Popovic A, Brown G, Chernikova TN, Garcia-Moyano A, Bjergah GE, Perez-Garcia P, Hai T, Del Pozo MV, Stokke R, Steen IH, Cui H, Xu X, Nocek BP, Alcaide M, Distaso M, Mesa V, Pelaez AI, Sanchez J, Buchholz PCF, Pleiss J, Fernandez-Guerra A, Glockner FO, Golyshina OV, Yakimov MM, Savchenko A, Jaeger KE, Yakunin AF, Streit WR, Golyshin PN, Guallar V, Ferrer M (2018)
Determinants and prediction of esterase substrate promiscuity patterns
ACS Chemical Biology 13: 225

Santiago G, Martinez-Martinez M, Alonso S, Bargiela R, Coscolin C, Golyshin PN, Guallar V, Ferrer M (2018)
Rational Engineering of Multiple Active Sites in an Ester Hydrolase
Biochemistry 57: 2245

Popovic A, Hai T, Tchigvintsev A, Hajighasemi M, Nocek B, Khusnutdinova AN, Brown G, Glinos J, Flick R, Skarina T, Chernikova TN, Yim V, Bruls T, Paslier DL, Yakimov MM, Joachimiak A, Ferrer M, Golyshina OV, Savchenko A, Golyshin PN, Yakunin AF (2017)
Activity screening of environmental metagenomic libraries reveals novel carboxylesterase families
Sci Rep 7: 44103

Hajighasemi M, Nocek BP, Tchigvintsev A, Brown G, Flick R, Xu X, Cui H, Hai T, Joachimiak A, Golyshin PN, Savchenko A, Edwards EA, Yakunin AF (2016)
Biochemical and Structural Insights into Enzymatic Depolymerization of Polylactic Acid and Other Polyesters by Microbial Carboxylesterases
Biomacromolecules 17: 2027

Alcaide M, Stogios PJ, Lafraya A, Tchigvintsev A, Flick R, Bargiela R, Chernikova TN, Reva ON, Hai T, Leggewie CC, Katzke N, La Cono V, Matesanz R, Jebbar M, Jaeger KE, Yakimov MM, Yakunin AF, Golyshin PN, Golyshina OV, Savchenko A, Ferrer M (2015)
Pressure adaptation is linked to thermal adaptation in salt-saturated marine habitats
Environ Microbiol 17: 332

Placido A, Hai T, Ferrer M, Chernikova TN, Distaso M, Armstrong D, Yakunin AF, Toshchakov SV, Yakimov MM, Kublanov IV, Golyshina OV, Pesole G, Ceci LR, Golyshin PN (2015)
Diversity of hydrolases from hydrothermal vent sediments of the Levante Bay, Vulcano Island (Aeolian archipelago) identified by activity-based metagenomics and biochemical characterization of new esterases and an arabinopyranosidase
Applied Microbiology & Biotechnology 99: 10031

Prive F, Newbold CJ, Kaderbhai NN, Girdwood SG, Golyshina OV, Golyshin PN, Scollan ND, Huws SA (2015)
Isolation and characterization of novel lipases/esterases from a bovine rumen metagenome
Applied Microbiology & Biotechnology 99: 5475

Tchigvintsev A, Tran H, Popovic A, Kovacic F, Brown G, Flick R, Hajighasemi M, Egorova O, Somody JC, Tchigvintsev D, Khusnutdinova A, Chernikova TN, Golyshina OV, Yakimov MM, Savchenko A, Golyshin PN, Jaeger KE, Yakunin AF (2015)
The environment shapes microbial enzymes: five cold-active and salt-resistant carboxylesterases from marine metagenomes
Applied Microbiology & Biotechnology 99: 2165

Alcaide M, Tornes J, Stogios PJ, Xu X, Gertler C, Di Leo R, Bargiela R, Lafraya A, Guazzaroni ME, Lopez-Cortes N, Chernikova TN, Golyshina OV, Nechitaylo TY, Plumeier I, Pieper DH, Yakimov MM, Savchenko A, Golyshin PN, Ferrer M (2013)
Single residues dictate the co-evolution of dual esterases: MCP hydrolases from the alpha/beta hydrolase family
Biochemical Journal 454: 157

Golyshin PN, Werner J, Chernikova TN, Tran H, Ferrer M, Yakimov MM, Teeling H, Golyshina OV (2013)
Genome Sequence of Thalassolituus oleivorans MIL-1 (DSM 14913T)
Genome Announc 1: e0014113

Kube M, Chernikova TN, Al-Ramahi Y, Beloqui A, Lopez-Cortez N, Guazzaroni ME, Heipieper HJ, Klages S, Kotsyurbenko OR, Langer I, Nechitaylo TY, Lunsdorf H, Fernandez M, Juarez S, Ciordia S, Singer A, Kagan O, Egorova O, Petit PA, Stogios P, Kim Y, Tchigvintsev A, Flick R, Denaro R, Genovese M, Albar JP, Reva ON, Martinez-Gomariz M, Tran H, Ferrer M, Savchenko A, Yakunin AF, Yakimov MM, Golyshina OV, Reinhardt R, Golyshin PN (2013)
Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica
Nat Commun 4: 2156

Alcaide M, Messina E, Richter M, Bargiela R, Peplies J, Huws SA, Newbold CJ, Golyshin PN, Simon MA, Lopez G, Yakimov MM, Ferrer M (2012)
Gene sets for utilization of primary and secondary nutrition supplies in the distal gut of endangered Iberian lynx
PLoS ONE 7: e51521

Del Pozo MV, Fernandez-Arrojo L, Gil-Martinez J, Montesinos A, Chernikova TN, Nechitaylo TY, Waliszek A, Tortajada M, Rojas A, Huws SA, Golyshina OV, Newbold CJ, Polaina J, Ferrer M, Golyshin PN (2012)
Microbial beta-glucosidases from cow rumen metagenome enhance the saccharification of lignocellulose in combination with commercial cellulase cocktail
Biotechnol Biofuels 5: 73

Lemak S, Tchigvintsev A, Petit P, Flick R, Singer AU, Brown G, Evdokimova E, Egorova O, Gonzalez CF, Chernikova TN, Yakimov MM, Kube M, Reinhardt R, Golyshin PN, Savchenko A, Yakunin AF (2012)
Structure and activity of the cold-active and anion-activated carboxyl esterase OLEI01171 from the oil-degrading marine bacterium Oleispira antarctica
Biochemical Journal 445: 193

Beloqui A, Polaina J, Vieites JM, Reyes-Duarte D, Torres R, Golyshina OV, Chernikova TN, Waliczek A, Aharoni A, Yakimov MM, Timmis KN, Golyshin PN, Ferrer M (2010)
Novel hybrid esterase-haloacid dehalogenase enzyme
Chembiochem 11: 1975

Beloqui A, Nechitaylo TY, Lopez-Cortes N, Ghazi A, Guazzaroni ME, Polaina J, Strittmatter AW, Reva O, Waliczek A, Yakimov MM, Golyshina OV, Ferrer M, Golyshin PN (2010)
Diversity of glycosyl hydrolases from cellulose-depleting communities enriched from casts of two earthworm species
Applied Environmental Microbiology 76: 5934

Vieites JM, Ghazi A, Beloqui A, Polaina J, Andreu JM, Golyshina OV, Nechitaylo TY, Waliczek A, Yakimov MM, Golyshin PN, Ferrer M (2010)
Inter-conversion of catalytic abilities in a bifunctional carboxyl/feruloyl-esterase from earthworm gut metagenome
Microb Biotechnol 3: 48

Golyshina OV, Golyshin PN, Timmis KN, Ferrer M (2006)
The 'pH optimum anomaly' of intracellular enzymes of Ferroplasma acidiphilum
Environ Microbiol 8: 416

Schneiker S, Martins dos Santos VA, Bartels D, Bekel T, Brecht M, Buhrmester J, Chernikova TN, Denaro R, Ferrer M, Gertler C, Goesmann A, Golyshina OV, Kaminski F, Khachane AN, Lang S, Linke B, McHardy AC, Meyer F, Nechitaylo T, Puhler A, Regenhardt D, Rupp O, Sabirova JS, Selbitschka W, Yakimov MM, Timmis KN, Vorholter FJ, Weidner S, Kaiser O, Golyshin PN (2006)
Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis
Nat Biotechnol 24: 997

Ferrer M, Golyshina OV, Chernikova TN, Khachane AN, Martins dos Santos VA, Yakimov MM, Timmis KN, Golyshin PN (2005)
Microbial enzymes mined from the Urania deep-sea hypersaline anoxic basin
Chemical Biology 12: 895

Ferrer M, Golyshina OV, Chernikova TN, Khachane AN, Reyes-Duarte D, Santos VA, Strompl C, Elborough K, Jarvis G, Neef A, Yakimov MM, Timmis KN, Golyshin PN (2005)
Novel hydrolase diversity retrieved from a metagenome library of bovine rumen microflora
Environ Microbiol 7: 1996

Reyes-Duarte D, Polaina J, Lopez-Cortes N, Alcalde M, Plou FJ, Elborough K, Ballesteros A, Timmis KN, Golyshin PN, Ferrer M (2005)
Conversion of a carboxylesterase into a triacylglycerol lipase by a random mutation
Angew Chem Int Ed Engl 44: 7553

Yakimov MM, Denaro R, Genovese M, Cappello S, D'Auria G, Chernikova TN, Timmis KN, Golyshin PN, Giluliano L (2005)
Natural microbial diversity in superficial sediments of Milazzo Harbor (Sicily) and community successions during microcosm enrichment with various hydrocarbons
Environ Microbiol 7: 1426

Ferrer M, Chernikova TN, Timmis KN, Golyshin PN (2004)
Expression of a temperature-sensitive esterase in a novel chaperone-based Escherichia coli strain
Applied Environmental Microbiology 70: 4499

Yakimov MM, Kroeger A, Slepak TN, Giuliano L, Timmis KN, Golyshin PN (1998)
A putative lichenysin A synthetase operon in B.licheniformis: initial charachterization.
Biochimica & Biophysica Acta 1399: 141