Jackson C, Toth-Petroczy A, Kolodny R, Hollfelder F, Fuxreiter M, Caroline Lynn Kamerlin S, Tokuriki N (2022)
Adventures on the routes of protein evolution - in memoriam Dan Salah Tawfik (1955 - 2021)
Journal of Molecular Biology

Schnettler JD, Klein OJ, Kaminski TS, Colin PY, Hollfelder F (2022)
Ultrahigh-Throughput Directed Evolution of a Metal-Free alpha/beta-Hydrolase with a Cys-His-Asp Triad into an Efficient Phosphotriesterase
Journal of the American Chemical Society

Schnettler-Fernandez D, Klein OJ, Kaminski TS, Colin PY, Hollfelder F (2022)
Ultrahigh-throughput directed evolution of a metal-free alpha/beta-hydrolase with a Cys-His-Asp triad into an efficient phosphotriesterase
Biorxiv

Schenkmayerova A, Pinto GP, Toul M, Marek M, Hernychova L, Planas-Iglesias J, Daniel Liskova V, Pluskal D, Vasina M, Emond S, Dorr M, Chaloupkova R, Bednar D, Prokop Z, Hollfelder F, Bornscheuer UT, Damborsky J (2021)
Engineering the protein dynamics of an ancestral luciferase
Nat Commun 12: 3616

Schenkmayerova A, Pinto GP, Toul M, Marek M, Hernychova L, Planas-Iglesias J, Liskova V, Pluskal D, Vasina M, Emond S, Dorr M, Chaloupkova R, Bednar D, Prokop Z, Hollfelder F, Bornscheuer UT, Damborsky J (2020)
Engineering Protein Dynamics of Ancestral Luciferase
Chemrxiv

Buryska T, Vasina M, Gielen F, Vanacek P, van Vliet L, Jezek J, Pilat Z, Zemanek P, Damborsky J, Hollfelder F, Prokop Z (2019)
Controlled Oil/Water Partitioning of Hydrophobic Substrates Extending the Bioanalytical Applications of Droplet-Based Microfluidics
Analytical Chemistry 91: 10008

van Loo B, Berry R, Boonyuen U, Mohamed MF, Golicnik M, Hengge AC, Hollfelder F (2019)
Transition-State Interactions in a Promiscuous Enzyme: Sulfate and Phosphate Monoester Hydrolysis by Pseudomonas aeruginosa Arylsulfatase
Biochemistry 58: 1363

Bayer CD, van Loo B, Hollfelder F (2017)
Specificity Effects of Amino Acid Substitutions in Promiscuous Hydrolases: Context-Dependence of Catalytic Residue Contributions to Local Fitness Landscapes in Nearby Sequence Space
Chembiochem 18: 1001

Campbell EC, Kaltenbach M, Correy GJ, Carr PD, Porebski BT, Livingstone EK, Afriat-Jurnou L, Buckle AM, Weik M, Hollfelder F, Tokuriki N, Jackson CJ (2016)
The role of protein dynamics in the evolution of new enzyme function
Nat Chemical Biology 12: 944

Kaltenbach M, Emond S, Hollfelder F, Tokuriki N (2016)
Functional Trade-Offs in Promiscuous Enzymes Cannot Be Explained by Intrinsic Mutational Robustness of the Native Activity
PLoS Genet 12: e1006305

Colin PY, Kintses B, Gielen F, Miton CM, Fischer G, Mohamed MF, Hyvonen M, Morgavi DP, Janssen DB, Hollfelder F (2015)
Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics
Nat Commun 6: 10008

Shafee T, Gatti-Lafranconi P, Minter R, Hollfelder F (2015)
Handicap-Recover Evolution Leads to a Chemically Versatile, Nucleophile-Permissive Protease
Chembiochem 16: 1866

Fischlechner M, Schaerli Y, Mohamed MF, Patil S, Abell C, Hollfelder F (2014)
Evolution of enzyme catalysts caged in biomimetic gel-shell beads
Nat Chem 6: 791

Theberge AB, Courtois F, Schaerli Y, Fischlechner M, Abell C, Hollfelder F, Huck WT (2010)
Microdroplets in microfluidics: an evolving platform for discoveries in chemistry and biology
Angew Chem Int Ed Engl 49: 5846

Leemhuis H, Stein V, Griffiths AD, Hollfelder F (2005)
New genotype-phenotype linkages for directed evolution of functional proteins
Current Opinion in Structural Biology 15: 472