Dittrich J, Popara M, Kubiak J, Dimura M, Schepers B, Verma N, Schmitz B, Dollinger P, Kovacic F, Jaeger KE, Seidel CAM, Peulen TO, Gohlke H (2023)
Resolution of Maximum Entropy Method-Derived Posterior Conformational Ensembles of a Flexible System Probed by FRET and Molecular Dynamics Simulations
J Chem Theory Comput
Davari MD, Cui H, Vedder M, Zhang L, Jaeger KE, Schwaneberg U (2022)
Polar substitutions on the surface of a lipase substantially improve tolerance in organic solvents
ChemSusChem
El Harrar T, Davari MD, Jaeger KE, Schwaneberg U, Gohlke H (2022)
Critical assessment of structure-based approaches to improve protein resistance in aqueous ionic liquids by enzyme-wide saturation mutagenesis
Comput Struct Biotechnol J 20: 399
Muller C, Bakkes PJ, Lenz P, Waffenschmidt V, Helleckes LM, Jaeger KE, Wiechert W, Knapp A, Freudl R, Oldiges M (2022)
Accelerated strain construction and characterization of C. glutamicum protein secretion by laboratory automation
Applied Microbiology & Biotechnology 106: 4481
Ahmad S, Strunk CH, Schott-Verdugo SN, Jaeger KE, Kovacic F, Gohlke H (2021)
Substrate access mechanism in a novel membrane-bound 1 phospholipase A of Pseudomonas aeruginosa concordant with specificity and regioselectivity
Biorxiv
Bakkes PJ, Lenz P, Muller C, Bida A, Dohmen-Olma D, Knapp A, Oldiges M, Jaeger KE, Freudl R (2021)
Biosensor-Based Optimization of Cutinase Secretion by Corynebacterium glutamicum
Front Microbiol 12: 750150
Bleffert F, Granzin J, Caliskan M, Schott-Verdugo SN, Siebers M, Thiele B, Rahme L, Felgner S, Dormann P, Gohlke H, Batra-Safferling R, Jaeger KE, Kovacic F (2021)
Evidence for a bacterial Lands cycle phospholipase A: Structural and mechanistic insights into membrane phospholipid remodeling
Biorxiv
Cui H, Eltoukhy L, Zhang L, Markel U, Jaeger KE, Davari MD, Schwaneberg U (2021)
Less unfavorable salt-bridges on the enzyme surface results in more organic cosolvent resistance
Angew Chem Int Ed Engl
Cui H, Jaeger KE, Davari MD, Schwaneberg U (2021)
CompassR Yields Highly Organic-Solvent-Tolerant Enzymes through Recombination of Compatible Substitutions
Chemistry 27: 2789
Hoppner A, Bollinger A, Kobus S, Thies S, Coscolin C, Ferrer M, Jaeger KE, Smits SHJ (2021)
Crystal structures of a novel family IV esterase in free and substrate-bound form
Febs J 288: 3570
Nutschel C, Coscolin C, David B, Mulnaes D, Ferrer M, Jaeger KE, Gohlke H (2021)
Promiscuous Esterases Counterintuitively Are Less Flexible than Specific Ones
J Chem Inf Model
Bollinger A, Thies S, Knieps-Grunhagen E, Gertzen C, Kobus S, Hoppner A, Ferrer M, Gohlke H, Smits SHJ, Jaeger KE (2020)
A Novel Polyester Hydrolase From the Marine Bacterium Pseudomonas aestusnigri - Structural and Functional Insights
Front Microbiol 11: 114
Bollinger A, Molitor R, Thies S, Koch R, Coscolin C, Ferrer M, Jaeger KE (2020)
Organic-Solvent-Tolerant Carboxylic Ester Hydrolases for Organic Synthesis
Applied Environmental Microbiology 86: e00106
Bollinger A, Thies S, Katzke N, Jaeger KE (2020)
The biotechnological potential of marine bacteria in the novel lineage of Pseudomonas pertucinogena
Microb Biotechnol 13: 19
Molitor R, Bollinger A, Kubicki S, Loeschcke A, Jaeger KE, Thies S (2020)
Agar plate-based screening methods for the identification of polyester hydrolysis by Pseudomonas species
Microb Biotechnol 13: 274
Verma N, Dollinger P, Kovacic F, Jaeger KE, Gohlke H (2020)
The Membrane-Integrated Steric Chaperone Lif Facilitates Active Site Opening of Pseudomonas aeruginosa Lipase A
J Comput Chem 41: 500
Viegas A, Dollinger P, Verma N, Kubiak J, Viennet T, Seidel CAM, Gohlke H, Etzkorn M, Kovacic F, Jaeger KE (2020)
Structural and dynamic insights revealing how lipase binding domain MD1 of Pseudomonas aeruginosa foldase affects lipase activation
Sci Rep 10: 3578
Volkenborn K, Kuschmierz L, Benz N, Lenz P, Knapp A, Jaeger KE (2020)
The length of ribosomal binding site spacer sequence controls the production yield for intracellular and secreted proteins by Bacillus subtilis
Microb Cell Fact 19: 154
Bleffert F, Granzin J, Gohlke H, Batra-Safferling R, Jaeger KE, Kovacic F (2019)
Pseudomonas aeruginosa esterase PA2949, a bacterial homolog of the human membrane esterase ABHD6: expression, purification and crystallization
Acta Crystallographica F Struct Biol Commun 75: 270
Kovacic F, Babic N, Krauss U, Jaeger KE (2019)
Classification of Lipolytic Enzymes from Bacteria
Handbook of Hydrocarbon and Lipid Microbiology Aerobic Utilization of Hydrocarbons, Oils, and Lipids: 255
Kubicki S, Bollinger A, Katzke N, Jaeger KE, Loeschcke A, Thies S (2019)
Marine Biosurfactants: Biosynthesis, Structural Diversity and Biotechnological Applications
Mar Drugs 17:
Weber J, Petrovic D, Strodel B, Smits SHJ, Kolkenbrock S, Leggewie C, Jaeger KE (2019)
Interaction of carbohydrate-binding modules with poly(ethylene terephthalate)
Applied Microbiology & Biotechnology 103: 4801
Martinez-Martinez M, Coscolin C, Santiago G, Chow J, Stogios PJ, Bargiela R, Gertler C, Navarro-Fernandez J, Bollinger A, Thies S, Mendez-Garcia C, Popovic A, Brown G, Chernikova TN, Garcia-Moyano A, Bjergah GE, Perez-Garcia P, Hai T, Del Pozo MV, Stokke R, Steen IH, Cui H, Xu X, Nocek BP, Alcaide M, Distaso M, Mesa V, Pelaez AI, Sanchez J, Buchholz PCF, Pleiss J, Fernandez-Guerra A, Glockner FO, Golyshina OV, Yakimov MM, Savchenko A, Jaeger KE, Yakunin AF, Streit WR, Golyshin PN, Guallar V, Ferrer M (2018)
Determinants and prediction of esterase substrate promiscuity patterns
ACS Chemical Biology 13: 225
Kaschner M, Schillinger O, Fettweiss T, Nutschel C, Krause F, Fulton A, Strodel B, Stadler A, Jaeger KE, Krauss U (2017)
A combination of mutational and computational scanning guides the design of an artificial ligand-binding controlled lipase
Sci Rep 7: 42592
Knapp A, Ripphahn M, Volkenborn K, Skoczinski P, Jaeger KE (2017)
Activity-independent screening of secreted proteins using split GFP
J Biotechnol 258: 110
Poehlein A, Daniel R, Thurmer A, Bollinger A, Thies S, Katzke N, Jaeger KE (2017)
First Insights into the Genome Sequence of Pseudomonas oleovorans DSM 1045
Genome Announc 5:
Kovacic F, Mandrysch A, Poojari C, Strodel B, Jaeger KE (2016)
Structural features determining thermal adaptation of esterases
Protein Engineering Des Sel 29: 65
Kovacic F, Bleffert F, Caliskan M, Wilhelm S, Granzin J, Batra-Safferling R, Jaeger KE (2016)
A membrane-bound esterase PA2949 from Pseudomonas aeruginosa is expressed and purified from Escherichia coli
FEBS Open Bio 6: 484
Alcaide M, Stogios PJ, Lafraya A, Tchigvintsev A, Flick R, Bargiela R, Chernikova TN, Reva ON, Hai T, Leggewie CC, Katzke N, La Cono V, Matesanz R, Jebbar M, Jaeger KE, Yakimov MM, Yakunin AF, Golyshin PN, Golyshina OV, Savchenko A, Ferrer M (2015)
Pressure adaptation is linked to thermal adaptation in salt-saturated marine habitats
Environ Microbiol 17: 332
Fulton A, Frauenkron-Machedjou VJ, Skoczinski P, Wilhelm S, Zhu L, Schwaneberg U, Jaeger KE (2015)
Exploring the Protein Stability Landscape: Bacillus subtilis Lipase A as a Model for Detergent Tolerance
Chembiochem 16: 930
Rahmen N, Fulton A, Ihling N, Magni M, Jaeger KE, Buchs J (2015)
Exchange of single amino acids at different positions of a recombinant protein affects metabolic burden in Escherichia coli
Microb Cell Fact 14: 10
Rathi PC, Jaeger KE, Gohlke H (2015)
Structural Rigidity and Protein Thermostability in Variants of Lipase A from Bacillus subtilis
PLoS ONE 10: e0130289
Tchigvintsev A, Tran H, Popovic A, Kovacic F, Brown G, Flick R, Hajighasemi M, Egorova O, Somody JC, Tchigvintsev D, Khusnutdinova A, Chernikova TN, Golyshina OV, Yakimov MM, Savchenko A, Golyshin PN, Jaeger KE, Yakunin AF (2015)
The environment shapes microbial enzymes: five cold-active and salt-resistant carboxylesterases from marine metagenomes
Applied Microbiology & Biotechnology 99: 2165
Buerth C, Kovacic F, Stock J, Terfruchte M, Wilhelm S, Jaeger KE, Feldbrugge M, Schipper K, Ernst JF, Tielker D (2014)
Uml2 is a novel CalB-type lipase of Ustilago maydis with phospholipase A activity
Applied Microbiology & Biotechnology 98: 4963
Tielen P, Kuhn H, Rosenau F, Jaeger KE, Flemming HC, Wingender J (2013)
Interaction between extracellular lipase LipA and the polysaccharide alginate of Pseudomonas aeruginosa
BMC Microbiol 13: 159
Chow J, Kovacic F, Dall Antonia Y, Krauss U, Fersini F, Schmeisser C, Lauinger B, Bongen P, Pietruszka J, Schmidt M, Menyes I, Bornscheuer UT, Eckstein M, Thum O, Liese A, Mueller-Dieckmann J, Jaeger KE, Streit WR (2012)
The metagenome-derived enzymes LipS and LipT increase the diversity of known lipases
PLoS ONE 7: e47665
Perez D, Kovacic F, Wilhelm S, Jaeger KE, Garcia MT, Ventosa A, Mellado E (2012)
Identification of amino acids involved in the hydrolytic activity of lipase LipBL from Marinobacter lipolyticus
Microbiology 158: 2192
Troeschel SC, Thies S, Link O, Real CI, Knops K, Wilhelm S, Rosenau F, Jaeger KE (2012)
Novel broad host range shuttle vectors for expression in Escherichia coli, Bacillus subtilis and Pseudomonas putida
J Biotechnol 161: 71
Franken B, Eggert T, Jaeger KE, Pohl M (2011)
Mechanism of acetaldehyde-induced deactivation of microbial lipases
BMC Biochem 12: 10
Funken H, Knapp A, Vasil ML, Wilhelm S, Jaeger KE, Rosenau F (2011)
The lipase LipA (PA2862) but not LipC (PA4813) from Pseudomonas aeruginosa influences regulation of pyoverdine production and expression of the sigma factor PvdS
Journal of Bacteriology 193: 5858
Fernandez-Alvaro E, Kourist R, Winter J, Bottcher D, Liebeton K, Naumer C, Eck J, Leggewie C, Jaeger KE, Streit WR, Bornscheuer UT (2010)
Enantioselective kinetic resolution of phenylalkyl carboxylic acids using metagenome-derived esterases
Microb Biotechnol 3: 59
Hausmann S, Jaeger KE (2010)
Lipolytic Enzymes from Bacteria
Handbook of Hydrocarbon and Lipid Microbiology Aerobic Utilization of Hydrocarbons, Oils, and Lipids: 1099
Krauss U, Lee J, Benkovic SJ, Jaeger KE (2010)
LOVely enzymes - towards engineering light-controllable biocatalysts
Microb Biotechnol 3: 15
Lescic Asler I, Ivic N, Kovacic F, Schell S, Knorr J, Krauss U, Wilhelm S, Kojic-Prodic B, Jaeger KE (2010)
Probing enzyme promiscuity of SGNH hydrolases
Chembiochem 11: 2158
Rosenau F, Isenhardt S, Gdynia A, Tielker D, Schmidt E, Tielen P, Schobert M, Jahn D, Wilhelm S, Jaeger KE (2010)
Lipase LipC affects motility, biofilm formation and rhamnolipid production in Pseudomonas aeruginosa
FEMS Microbiology Letters 309: 25
Hausmann S, Wilhelm S, Jaeger KE, Rosenau F (2008)
Mutations towards enantioselectivity adversely affect secretion of Pseudomonas aeruginosa lipase
FEMS Microbiology Letters 282: 65
Becker S, Michalczyk A, Wilhelm S, Jaeger KE, Kolmar H (2007)
Ultrahigh-throughput screening to identify E. coli cells expressing functionally active enzymes on their surface
Chembiochem 8: 943
Meier R, Drepper T, Svensson V, Jaeger KE, Baumann U (2007)
A calcium-gated lid and a large beta-roll sandwich are revealed by the crystal structure of extracellular lipase from Serratia marcescens
Journal of Biological Chemistry 282: 31477
Reetz MT, Puls M, Carballeira JD, Vogel A, Jaeger KE, Eggert T, Thiel W, Bocola M, Otte N (2007)
Learning from directed evolution: Further lessons from theoretical investigations into cooperative mutations in lipase enantioselectivity
Chembiochem 8: 106
Wilhelm S, Rosenau F, Becker S, Buest S, Hausmann S, Kolmar H, Jaeger KE (2007)
Functional cell-surface display of a lipase-specific chaperone
Chembiochem 8: 55
Detry J, Rosenbaum T, Lutz S, Hahn D, Jaeger KE, Muller M, Eggert T (2006)
Biocatalytic production of enantiopure cyclohexane-trans-1,2-diol using extracellular lipases from Bacillus subtilis
Applied Microbiology & Biotechnology 72: 1107
Elend C, Schmeisser C, Leggewie C, Babiak P, Carballeira JD, Steele HL, Reymond JL, Jaeger KE, Streit WR (2006)
Isolation and biochemical characterization of two novel metagenome-derived esterases
Applied Environmental Microbiology 72: 3637
Henning H, Leggewie C, Pohl M, Muller M, Eggert T, Jaeger KE (2006)
Identification of novel benzoylformate decarboxylases by growth selection
Applied Environmental Microbiology 72: 7510
Becker S, Theile S, Heppeler N, Michalczyk A, Wentzel A, Wilhelm S, Jaeger KE, Kolmar H (2005)
A generic system for the Escherichia coli cell-surface display of lipolytic enzymes
FEBS Letters 579: 1177
Eggert T, Funke SA, Rao NM, Acharya P, Krumm H, Reetz MT, Jaeger KE (2005)
Multiplex-PCR-based recombination as a novel high-fidelity method for directed evolution
Chembiochem 6: 1062
Funke SA, Otte N, Eggert T, Bocola M, Jaeger KE, Thiel W (2005)
Combination of computational prescreening and experimental library construction can accelerate enzyme optimization by directed evolution
Protein Engineering Des Sel 18: 509
Bocola M, Otte N, Jaeger KE, Reetz MT, Thiel W (2004)
Learning from directed evolution: theoretical investigations into cooperative mutations in lipase enantioselectivity
Chembiochem 5: 214
Jaeger KE, Eggert T (2004)
Enantioselective biocatalysis optimized by directed evolution
Curr Opin Biotechnol 15: 305
Rosenau F, Tommassen J, Jaeger KE (2004)
Lipase-specific foldases
Chembiochem 5: 152
Streit WR, Daniel R, Jaeger KE (2004)
Prospecting for biocatalysts and drugs in the genomes of non-cultured microorganisms
Curr Opin Biotechnol 15: 285
Eggert T, Brockmeier U, Droge MJ, Quax WJ, Jaeger KE (2003)
Extracellular lipases from Bacillus subtilis: regulation of gene expression and enzyme activity by amino acid supply and external pH
FEMS Microbiology Letters 225: 319
Schmeisser C, Stockigt C, Raasch C, Wingender J, Timmis KN, Wenderoth DF, Flemming HC, Liesegang H, Schmitz RA, Jaeger KE, Streit WR (2003)
Metagenome survey of biofilms in drinking-water networks
Applied Environmental Microbiology 69: 7298
Voget S, Leggewie C, Uesbeck A, Raasch C, Jaeger KE, Streit WR (2003)
Prospecting for novel biocatalysts in a soil metagenome
Applied Environmental Microbiology 69: 6235
Jaeger KE, Eggert T (2002)
Lipases for biotechnology
Curr Opin Biotechnol 13: 390
Eggert T, van Pouderoyen G, Dijkstra BW, Jaeger KE (2001)
Lipolytic enzymes LipA and LipB from Bacillus subtilis differ in regulation of gene expression, biochemical properties, and three-dimensional structure
FEBS Letters 502: 89
Jaeger KE, Eggert T, Eipper A, Reetz MT (2001)
Directed evolution and the creation of enantioselective biocatalysts
Applied Microbiology & Biotechnology 55: 519
Liebeton K, Zacharias A, Jaeger KE (2001)
Disulfide bond in Pseudomonas aeruginosa lipase stabilizes the structure but is not required for interaction with its foldase
Journal of Bacteriology 183: 597
Reetz MT, Wilensek S, Zha D, Jaeger KE (2001)
Directed Evolution of an Enantioselective Enzyme through Combinatorial Multiple-Cassette Mutagenesis
Angew Chem Int Ed Engl 40: 3589
van Pouderoyen G, Eggert T, Jaeger KE, Dijkstra BW (2001)
The crystal structure of Bacillus subtilis lipase: a minimal alpha/beta hydrolase fold enzyme
Journal of Molecular Biology 309: 215
Eggert T, Pencreac'h G, Douchet I, Verger R, Jaeger KE (2000)
A novel extracellular esterase from Bacillus subtilis and its conversion to a monoacylglycerol hydrolase
European Journal of Biochemistry 267: 6459
Jaeger KE, Reetz MT (2000)
Directed evolution of enantioselective enzymes for organic chemistry
Curr Opin Chemical Biology 4: 68
Liebeton K, Zonta A, Schimossek K, Nardini M, Lang D, Dijkstra BW, Reetz MT, Jaeger KE (2000)
Directed evolution of an enantioselective lipase
Chemical Biology 7: 709
Nardini M, Lang DA, Liebeton K, Jaeger KE, Dijkstra BW (2000)
Crystal structure of pseudomonas aeruginosa lipase in the open conformation. The prototype for family I.1 of bacterial lipases
Journal of Biological Chemistry 275: 31219
Reetz MT, Jaeger KE (2000)
Enantioselective enzymes for organic synthesis created by directed evolution
Chemistry 6: 407
Windgassen M, Urban A, Jaeger KE (2000)
Rapid gene inactivation in Pseudomonas aeruginosa
FEMS Microbiology Letters 193: 201
Arpigny JL, Jaeger KE (1999)
Bacterial lipolytic enzymes: classification and properties
Biochemical Journal 343: 177
El Khattabi M, Ockhuijsen C, Bitter W, Jaeger KE, Tommassen J (1999)
Specificity of the lipase-specific foldases of gram-negative bacteria and the role of the membrane anchor
Molecular & General Genetics 261: 770
Jaeger KE, Dijkstra BW, Reetz MT (1999)
Bacterial biocatalysts: molecular biology, three-dimensional structures, and biotechnological applications of lipases
Annu Rev Microbiol 53: 315
Wilhelm S, Tommassen J, Jaeger KE (1999)
A novel lipolytic enzyme located in the outer membrane of Pseudomonas aeruginosa
Journal of Bacteriology 181: 6977
Arpigny JL, Jendrossek D, Jaeger KE (1998)
A novel heat-stable lipolytic enzyme from Sulfolobus acidocaldarius DSM 639 displaying similarity to polyhydroxyalkanoate depolymerases
FEMS Microbiology Letters 167: 69
Jaeger KE, Reetz MT (1998)
Microbial lipases form versatile tools for biotechnology
Trends Biotechnol 16: 396
Reetz MT, Jaeger KE (1998)
Overexpression, immobilization and biotechnological application of Pseudomonas lipases
Chemistry & Physic of Lipids 93: 3
Konig B, Jaeger KE, Sage AE, Vasil ML, Konig W (1996)
Role of Pseudomonas aeruginosa lipase in inflammatory mediator release from human inflammatory effector cells (platelets, granulocytes, and monocytes
Infect Immun 64: 3252
Winteler HV, Schneidinger B, Jaeger KE, Haas D (1996)
Anaerobically controlled expression system derived from the arcDABC operon of Pseudomonas aeruginosa: application to lipase production
Applied Environmental Microbiology 62: 3391
Jaeger KE, Steinbuchel A, Jendrossek D (1995)
Substrate specificities of bacterial polyhydroxyalkanoate depolymerases and lipases: bacterial lipases hydrolyze poly(omega-hydroxyalkanoates)
Applied Environmental Microbiology 61: 3113
Li X, Tetling S, Winkler UK, Jaeger KE, Benedik MJ (1995)
Gene cloning, sequence analysis, purification, and secretion by Escherichia coli of an extracellular lipase from Serratia marcescens
Applied Environmental Microbiology 61: 2674
Taipa MA, Liebeton K, Costa JV, Cabral JM, Jaeger KE (1995)
Lipase from Chromobacterium viscosum: biochemical characterization indicating homology to the lipase from Pseudomonas glumae
Biochimica & Biophysica Acta 1256: 396
Jaeger KE, Ransac S, Dijkstra BW, Colson C, van Heuvel M, Misset O (1994)
Bacterial lipases
FEMS Microbiology Reviews 15: 29
Misset O, Gerritse G, Jaeger KE, Winkler U, Colson C, Schanck K, Lesuisse E, Dartois V, Blaauw M, Ransac S, et al. (1994)
The structure-function relationship of the lipases from Pseudomonas aeruginosa and Bacillus subtilis
Protein Engineering 7: 523
Jaeger KE, Ransac S, Koch HB, Ferrato F, Dijkstra BW (1993)
Topological characterization and modeling of the 3D structure of lipase from Pseudomonas aeruginosa
FEBS Letters 332: 143
Jaeger KE, Adrian FJ, Meyer HE, Hancock RE, Winkler UK (1992)
Extracellular lipase from Pseudomonas aeruginosa is an amphiphilic protein
Biochimica & Biophysica Acta 1120: 315
Jaeger KE, Kharazmi A, Hoiby N (1991)
Extracellular lipase of Pseudomonas aeruginosa: biochemical characterization and effect on human neutrophil and monocyte function in vitro
Microb Pathog 10: 173
Kouker G, Jaeger KE (1987)
Specific and sensitive plate assay for bacterial lipases
Applied Environmental Microbiology 53: 211
Stuer W, Jaeger KE, Winkler UK (1986)
Purification of extracellular lipase from Pseudomonas aeruginosa
Journal of Bacteriology 168: 1070