Dittrich J, Popara M, Kubiak J, Dimura M, Schepers B, Verma N, Schmitz B, Dollinger P, Kovacic F, Jaeger KE, Seidel CAM, Peulen TO, Gohlke H (2023)
Resolution of Maximum Entropy Method-Derived Posterior Conformational Ensembles of a Flexible System Probed by FRET and Molecular Dynamics Simulations
J Chem Theory Comput

Davari MD, Cui H, Vedder M, Zhang L, Jaeger KE, Schwaneberg U (2022)
Polar substitutions on the surface of a lipase substantially improve tolerance in organic solvents

El Harrar T, Davari MD, Jaeger KE, Schwaneberg U, Gohlke H (2022)
Critical assessment of structure-based approaches to improve protein resistance in aqueous ionic liquids by enzyme-wide saturation mutagenesis
Comput Struct Biotechnol J 20: 399

Muller C, Bakkes PJ, Lenz P, Waffenschmidt V, Helleckes LM, Jaeger KE, Wiechert W, Knapp A, Freudl R, Oldiges M (2022)
Accelerated strain construction and characterization of C. glutamicum protein secretion by laboratory automation
Applied Microbiology & Biotechnology 106: 4481

Ahmad S, Strunk CH, Schott-Verdugo SN, Jaeger KE, Kovacic F, Gohlke H (2021)
Substrate access mechanism in a novel membrane-bound 1 phospholipase A of Pseudomonas aeruginosa concordant with specificity and regioselectivity

Bakkes PJ, Lenz P, Muller C, Bida A, Dohmen-Olma D, Knapp A, Oldiges M, Jaeger KE, Freudl R (2021)
Biosensor-Based Optimization of Cutinase Secretion by Corynebacterium glutamicum
Front Microbiol 12: 750150

Bleffert F, Granzin J, Caliskan M, Schott-Verdugo SN, Siebers M, Thiele B, Rahme L, Felgner S, Dormann P, Gohlke H, Batra-Safferling R, Jaeger KE, Kovacic F (2021)
Evidence for a bacterial Lands cycle phospholipase A: Structural and mechanistic insights into membrane phospholipid remodeling

Cui H, Eltoukhy L, Zhang L, Markel U, Jaeger KE, Davari MD, Schwaneberg U (2021)
Less unfavorable salt-bridges on the enzyme surface results in more organic cosolvent resistance
Angew Chem Int Ed Engl

Cui H, Jaeger KE, Davari MD, Schwaneberg U (2021)
CompassR Yields Highly Organic-Solvent-Tolerant Enzymes through Recombination of Compatible Substitutions
Chemistry 27: 2789

Hoppner A, Bollinger A, Kobus S, Thies S, Coscolin C, Ferrer M, Jaeger KE, Smits SHJ (2021)
Crystal structures of a novel family IV esterase in free and substrate-bound form
Febs J 288: 3570

Nutschel C, Coscolin C, David B, Mulnaes D, Ferrer M, Jaeger KE, Gohlke H (2021)
Promiscuous Esterases Counterintuitively Are Less Flexible than Specific Ones
J Chem Inf Model

Bollinger A, Thies S, Knieps-Grunhagen E, Gertzen C, Kobus S, Hoppner A, Ferrer M, Gohlke H, Smits SHJ, Jaeger KE (2020)
A Novel Polyester Hydrolase From the Marine Bacterium Pseudomonas aestusnigri - Structural and Functional Insights
Front Microbiol 11: 114

Bollinger A, Molitor R, Thies S, Koch R, Coscolin C, Ferrer M, Jaeger KE (2020)
Organic-Solvent-Tolerant Carboxylic Ester Hydrolases for Organic Synthesis
Applied Environmental Microbiology 86: e00106

Bollinger A, Thies S, Katzke N, Jaeger KE (2020)
The biotechnological potential of marine bacteria in the novel lineage of Pseudomonas pertucinogena
Microb Biotechnol 13: 19

Molitor R, Bollinger A, Kubicki S, Loeschcke A, Jaeger KE, Thies S (2020)
Agar plate-based screening methods for the identification of polyester hydrolysis by Pseudomonas species
Microb Biotechnol 13: 274

Verma N, Dollinger P, Kovacic F, Jaeger KE, Gohlke H (2020)
The Membrane-Integrated Steric Chaperone Lif Facilitates Active Site Opening of Pseudomonas aeruginosa Lipase A
J Comput Chem 41: 500

Viegas A, Dollinger P, Verma N, Kubiak J, Viennet T, Seidel CAM, Gohlke H, Etzkorn M, Kovacic F, Jaeger KE (2020)
Structural and dynamic insights revealing how lipase binding domain MD1 of Pseudomonas aeruginosa foldase affects lipase activation
Sci Rep 10: 3578

Volkenborn K, Kuschmierz L, Benz N, Lenz P, Knapp A, Jaeger KE (2020)
The length of ribosomal binding site spacer sequence controls the production yield for intracellular and secreted proteins by Bacillus subtilis
Microb Cell Fact 19: 154

Bleffert F, Granzin J, Gohlke H, Batra-Safferling R, Jaeger KE, Kovacic F (2019)
Pseudomonas aeruginosa esterase PA2949, a bacterial homolog of the human membrane esterase ABHD6: expression, purification and crystallization
Acta Crystallographica F Struct Biol Commun 75: 270

Kovacic F, Babic N, Krauss U, Jaeger KE (2019)
Classification of Lipolytic Enzymes from Bacteria
Handbook of Hydrocarbon and Lipid Microbiology Aerobic Utilization of Hydrocarbons, Oils, and Lipids: 255

Kubicki S, Bollinger A, Katzke N, Jaeger KE, Loeschcke A, Thies S (2019)
Marine Biosurfactants: Biosynthesis, Structural Diversity and Biotechnological Applications
Mar Drugs 17:

Weber J, Petrovic D, Strodel B, Smits SHJ, Kolkenbrock S, Leggewie C, Jaeger KE (2019)
Interaction of carbohydrate-binding modules with poly(ethylene terephthalate)
Applied Microbiology & Biotechnology 103: 4801

Martinez-Martinez M, Coscolin C, Santiago G, Chow J, Stogios PJ, Bargiela R, Gertler C, Navarro-Fernandez J, Bollinger A, Thies S, Mendez-Garcia C, Popovic A, Brown G, Chernikova TN, Garcia-Moyano A, Bjergah GE, Perez-Garcia P, Hai T, Del Pozo MV, Stokke R, Steen IH, Cui H, Xu X, Nocek BP, Alcaide M, Distaso M, Mesa V, Pelaez AI, Sanchez J, Buchholz PCF, Pleiss J, Fernandez-Guerra A, Glockner FO, Golyshina OV, Yakimov MM, Savchenko A, Jaeger KE, Yakunin AF, Streit WR, Golyshin PN, Guallar V, Ferrer M (2018)
Determinants and prediction of esterase substrate promiscuity patterns
ACS Chemical Biology 13: 225

Kaschner M, Schillinger O, Fettweiss T, Nutschel C, Krause F, Fulton A, Strodel B, Stadler A, Jaeger KE, Krauss U (2017)
A combination of mutational and computational scanning guides the design of an artificial ligand-binding controlled lipase
Sci Rep 7: 42592

Knapp A, Ripphahn M, Volkenborn K, Skoczinski P, Jaeger KE (2017)
Activity-independent screening of secreted proteins using split GFP
J Biotechnol 258: 110

Poehlein A, Daniel R, Thurmer A, Bollinger A, Thies S, Katzke N, Jaeger KE (2017)
First Insights into the Genome Sequence of Pseudomonas oleovorans DSM 1045
Genome Announc 5:

Kovacic F, Mandrysch A, Poojari C, Strodel B, Jaeger KE (2016)
Structural features determining thermal adaptation of esterases
Protein Engineering Des Sel 29: 65

Kovacic F, Bleffert F, Caliskan M, Wilhelm S, Granzin J, Batra-Safferling R, Jaeger KE (2016)
A membrane-bound esterase PA2949 from Pseudomonas aeruginosa is expressed and purified from Escherichia coli
FEBS Open Bio 6: 484

Alcaide M, Stogios PJ, Lafraya A, Tchigvintsev A, Flick R, Bargiela R, Chernikova TN, Reva ON, Hai T, Leggewie CC, Katzke N, La Cono V, Matesanz R, Jebbar M, Jaeger KE, Yakimov MM, Yakunin AF, Golyshin PN, Golyshina OV, Savchenko A, Ferrer M (2015)
Pressure adaptation is linked to thermal adaptation in salt-saturated marine habitats
Environ Microbiol 17: 332

Fulton A, Frauenkron-Machedjou VJ, Skoczinski P, Wilhelm S, Zhu L, Schwaneberg U, Jaeger KE (2015)
Exploring the Protein Stability Landscape: Bacillus subtilis Lipase A as a Model for Detergent Tolerance
Chembiochem 16: 930

Rahmen N, Fulton A, Ihling N, Magni M, Jaeger KE, Buchs J (2015)
Exchange of single amino acids at different positions of a recombinant protein affects metabolic burden in Escherichia coli
Microb Cell Fact 14: 10

Rathi PC, Jaeger KE, Gohlke H (2015)
Structural Rigidity and Protein Thermostability in Variants of Lipase A from Bacillus subtilis
PLoS ONE 10: e0130289

Tchigvintsev A, Tran H, Popovic A, Kovacic F, Brown G, Flick R, Hajighasemi M, Egorova O, Somody JC, Tchigvintsev D, Khusnutdinova A, Chernikova TN, Golyshina OV, Yakimov MM, Savchenko A, Golyshin PN, Jaeger KE, Yakunin AF (2015)
The environment shapes microbial enzymes: five cold-active and salt-resistant carboxylesterases from marine metagenomes
Applied Microbiology & Biotechnology 99: 2165

Buerth C, Kovacic F, Stock J, Terfruchte M, Wilhelm S, Jaeger KE, Feldbrugge M, Schipper K, Ernst JF, Tielker D (2014)
Uml2 is a novel CalB-type lipase of Ustilago maydis with phospholipase A activity
Applied Microbiology & Biotechnology 98: 4963

Tielen P, Kuhn H, Rosenau F, Jaeger KE, Flemming HC, Wingender J (2013)
Interaction between extracellular lipase LipA and the polysaccharide alginate of Pseudomonas aeruginosa
BMC Microbiol 13: 159

Chow J, Kovacic F, Dall Antonia Y, Krauss U, Fersini F, Schmeisser C, Lauinger B, Bongen P, Pietruszka J, Schmidt M, Menyes I, Bornscheuer UT, Eckstein M, Thum O, Liese A, Mueller-Dieckmann J, Jaeger KE, Streit WR (2012)
The metagenome-derived enzymes LipS and LipT increase the diversity of known lipases
PLoS ONE 7: e47665

Perez D, Kovacic F, Wilhelm S, Jaeger KE, Garcia MT, Ventosa A, Mellado E (2012)
Identification of amino acids involved in the hydrolytic activity of lipase LipBL from Marinobacter lipolyticus
Microbiology 158: 2192

Troeschel SC, Thies S, Link O, Real CI, Knops K, Wilhelm S, Rosenau F, Jaeger KE (2012)
Novel broad host range shuttle vectors for expression in Escherichia coli, Bacillus subtilis and Pseudomonas putida
J Biotechnol 161: 71

Franken B, Eggert T, Jaeger KE, Pohl M (2011)
Mechanism of acetaldehyde-induced deactivation of microbial lipases
BMC Biochem 12: 10

Funken H, Knapp A, Vasil ML, Wilhelm S, Jaeger KE, Rosenau F (2011)
The lipase LipA (PA2862) but not LipC (PA4813) from Pseudomonas aeruginosa influences regulation of pyoverdine production and expression of the sigma factor PvdS
Journal of Bacteriology 193: 5858

Fernandez-Alvaro E, Kourist R, Winter J, Bottcher D, Liebeton K, Naumer C, Eck J, Leggewie C, Jaeger KE, Streit WR, Bornscheuer UT (2010)
Enantioselective kinetic resolution of phenylalkyl carboxylic acids using metagenome-derived esterases
Microb Biotechnol 3: 59

Hausmann S, Jaeger KE (2010)
Lipolytic Enzymes from Bacteria
Handbook of Hydrocarbon and Lipid Microbiology Aerobic Utilization of Hydrocarbons, Oils, and Lipids: 1099

Krauss U, Lee J, Benkovic SJ, Jaeger KE (2010)
LOVely enzymes - towards engineering light-controllable biocatalysts
Microb Biotechnol 3: 15

Lescic Asler I, Ivic N, Kovacic F, Schell S, Knorr J, Krauss U, Wilhelm S, Kojic-Prodic B, Jaeger KE (2010)
Probing enzyme promiscuity of SGNH hydrolases
Chembiochem 11: 2158

Rosenau F, Isenhardt S, Gdynia A, Tielker D, Schmidt E, Tielen P, Schobert M, Jahn D, Wilhelm S, Jaeger KE (2010)
Lipase LipC affects motility, biofilm formation and rhamnolipid production in Pseudomonas aeruginosa
FEMS Microbiology Letters 309: 25

Hausmann S, Wilhelm S, Jaeger KE, Rosenau F (2008)
Mutations towards enantioselectivity adversely affect secretion of Pseudomonas aeruginosa lipase
FEMS Microbiology Letters 282: 65

Becker S, Michalczyk A, Wilhelm S, Jaeger KE, Kolmar H (2007)
Ultrahigh-throughput screening to identify E. coli cells expressing functionally active enzymes on their surface
Chembiochem 8: 943

Meier R, Drepper T, Svensson V, Jaeger KE, Baumann U (2007)
A calcium-gated lid and a large beta-roll sandwich are revealed by the crystal structure of extracellular lipase from Serratia marcescens
Journal of Biological Chemistry 282: 31477

Reetz MT, Puls M, Carballeira JD, Vogel A, Jaeger KE, Eggert T, Thiel W, Bocola M, Otte N (2007)
Learning from directed evolution: Further lessons from theoretical investigations into cooperative mutations in lipase enantioselectivity
Chembiochem 8: 106

Wilhelm S, Rosenau F, Becker S, Buest S, Hausmann S, Kolmar H, Jaeger KE (2007)
Functional cell-surface display of a lipase-specific chaperone
Chembiochem 8: 55

Detry J, Rosenbaum T, Lutz S, Hahn D, Jaeger KE, Muller M, Eggert T (2006)
Biocatalytic production of enantiopure cyclohexane-trans-1,2-diol using extracellular lipases from Bacillus subtilis
Applied Microbiology & Biotechnology 72: 1107

Elend C, Schmeisser C, Leggewie C, Babiak P, Carballeira JD, Steele HL, Reymond JL, Jaeger KE, Streit WR (2006)
Isolation and biochemical characterization of two novel metagenome-derived esterases
Applied Environmental Microbiology 72: 3637

Henning H, Leggewie C, Pohl M, Muller M, Eggert T, Jaeger KE (2006)
Identification of novel benzoylformate decarboxylases by growth selection
Applied Environmental Microbiology 72: 7510

Becker S, Theile S, Heppeler N, Michalczyk A, Wentzel A, Wilhelm S, Jaeger KE, Kolmar H (2005)
A generic system for the Escherichia coli cell-surface display of lipolytic enzymes
FEBS Letters 579: 1177

Eggert T, Funke SA, Rao NM, Acharya P, Krumm H, Reetz MT, Jaeger KE (2005)
Multiplex-PCR-based recombination as a novel high-fidelity method for directed evolution
Chembiochem 6: 1062

Funke SA, Otte N, Eggert T, Bocola M, Jaeger KE, Thiel W (2005)
Combination of computational prescreening and experimental library construction can accelerate enzyme optimization by directed evolution
Protein Engineering Des Sel 18: 509

Bocola M, Otte N, Jaeger KE, Reetz MT, Thiel W (2004)
Learning from directed evolution: theoretical investigations into cooperative mutations in lipase enantioselectivity
Chembiochem 5: 214

Jaeger KE, Eggert T (2004)
Enantioselective biocatalysis optimized by directed evolution
Curr Opin Biotechnol 15: 305

Rosenau F, Tommassen J, Jaeger KE (2004)
Lipase-specific foldases
Chembiochem 5: 152

Streit WR, Daniel R, Jaeger KE (2004)
Prospecting for biocatalysts and drugs in the genomes of non-cultured microorganisms
Curr Opin Biotechnol 15: 285

Eggert T, Brockmeier U, Droge MJ, Quax WJ, Jaeger KE (2003)
Extracellular lipases from Bacillus subtilis: regulation of gene expression and enzyme activity by amino acid supply and external pH
FEMS Microbiology Letters 225: 319

Schmeisser C, Stockigt C, Raasch C, Wingender J, Timmis KN, Wenderoth DF, Flemming HC, Liesegang H, Schmitz RA, Jaeger KE, Streit WR (2003)
Metagenome survey of biofilms in drinking-water networks
Applied Environmental Microbiology 69: 7298

Voget S, Leggewie C, Uesbeck A, Raasch C, Jaeger KE, Streit WR (2003)
Prospecting for novel biocatalysts in a soil metagenome
Applied Environmental Microbiology 69: 6235

Jaeger KE, Eggert T (2002)
Lipases for biotechnology
Curr Opin Biotechnol 13: 390

Eggert T, van Pouderoyen G, Dijkstra BW, Jaeger KE (2001)
Lipolytic enzymes LipA and LipB from Bacillus subtilis differ in regulation of gene expression, biochemical properties, and three-dimensional structure
FEBS Letters 502: 89

Jaeger KE, Eggert T, Eipper A, Reetz MT (2001)
Directed evolution and the creation of enantioselective biocatalysts
Applied Microbiology & Biotechnology 55: 519

Liebeton K, Zacharias A, Jaeger KE (2001)
Disulfide bond in Pseudomonas aeruginosa lipase stabilizes the structure but is not required for interaction with its foldase
Journal of Bacteriology 183: 597

Reetz MT, Wilensek S, Zha D, Jaeger KE (2001)
Directed Evolution of an Enantioselective Enzyme through Combinatorial Multiple-Cassette Mutagenesis
Angew Chem Int Ed Engl 40: 3589

van Pouderoyen G, Eggert T, Jaeger KE, Dijkstra BW (2001)
The crystal structure of Bacillus subtilis lipase: a minimal alpha/beta hydrolase fold enzyme
Journal of Molecular Biology 309: 215

Eggert T, Pencreac'h G, Douchet I, Verger R, Jaeger KE (2000)
A novel extracellular esterase from Bacillus subtilis and its conversion to a monoacylglycerol hydrolase
European Journal of Biochemistry 267: 6459

Jaeger KE, Reetz MT (2000)
Directed evolution of enantioselective enzymes for organic chemistry
Curr Opin Chemical Biology 4: 68

Liebeton K, Zonta A, Schimossek K, Nardini M, Lang D, Dijkstra BW, Reetz MT, Jaeger KE (2000)
Directed evolution of an enantioselective lipase
Chemical Biology 7: 709

Nardini M, Lang DA, Liebeton K, Jaeger KE, Dijkstra BW (2000)
Crystal structure of pseudomonas aeruginosa lipase in the open conformation. The prototype for family I.1 of bacterial lipases
Journal of Biological Chemistry 275: 31219

Reetz MT, Jaeger KE (2000)
Enantioselective enzymes for organic synthesis created by directed evolution
Chemistry 6: 407

Windgassen M, Urban A, Jaeger KE (2000)
Rapid gene inactivation in Pseudomonas aeruginosa
FEMS Microbiology Letters 193: 201

Arpigny JL, Jaeger KE (1999)
Bacterial lipolytic enzymes: classification and properties
Biochemical Journal 343: 177

El Khattabi M, Ockhuijsen C, Bitter W, Jaeger KE, Tommassen J (1999)
Specificity of the lipase-specific foldases of gram-negative bacteria and the role of the membrane anchor
Molecular & General Genetics 261: 770

Jaeger KE, Dijkstra BW, Reetz MT (1999)
Bacterial biocatalysts: molecular biology, three-dimensional structures, and biotechnological applications of lipases
Annu Rev Microbiol 53: 315

Wilhelm S, Tommassen J, Jaeger KE (1999)
A novel lipolytic enzyme located in the outer membrane of Pseudomonas aeruginosa
Journal of Bacteriology 181: 6977

Arpigny JL, Jendrossek D, Jaeger KE (1998)
A novel heat-stable lipolytic enzyme from Sulfolobus acidocaldarius DSM 639 displaying similarity to polyhydroxyalkanoate depolymerases
FEMS Microbiology Letters 167: 69

Jaeger KE, Reetz MT (1998)
Microbial lipases form versatile tools for biotechnology
Trends Biotechnol 16: 396

Reetz MT, Jaeger KE (1998)
Overexpression, immobilization and biotechnological application of Pseudomonas lipases
Chemistry & Physic of Lipids 93: 3

Konig B, Jaeger KE, Sage AE, Vasil ML, Konig W (1996)
Role of Pseudomonas aeruginosa lipase in inflammatory mediator release from human inflammatory effector cells (platelets, granulocytes, and monocytes
Infect Immun 64: 3252

Winteler HV, Schneidinger B, Jaeger KE, Haas D (1996)
Anaerobically controlled expression system derived from the arcDABC operon of Pseudomonas aeruginosa: application to lipase production
Applied Environmental Microbiology 62: 3391

Jaeger KE, Steinbuchel A, Jendrossek D (1995)
Substrate specificities of bacterial polyhydroxyalkanoate depolymerases and lipases: bacterial lipases hydrolyze poly(omega-hydroxyalkanoates)
Applied Environmental Microbiology 61: 3113

Li X, Tetling S, Winkler UK, Jaeger KE, Benedik MJ (1995)
Gene cloning, sequence analysis, purification, and secretion by Escherichia coli of an extracellular lipase from Serratia marcescens
Applied Environmental Microbiology 61: 2674

Taipa MA, Liebeton K, Costa JV, Cabral JM, Jaeger KE (1995)
Lipase from Chromobacterium viscosum: biochemical characterization indicating homology to the lipase from Pseudomonas glumae
Biochimica & Biophysica Acta 1256: 396

Jaeger KE, Ransac S, Dijkstra BW, Colson C, van Heuvel M, Misset O (1994)
Bacterial lipases
FEMS Microbiology Reviews 15: 29

Misset O, Gerritse G, Jaeger KE, Winkler U, Colson C, Schanck K, Lesuisse E, Dartois V, Blaauw M, Ransac S, et al. (1994)
The structure-function relationship of the lipases from Pseudomonas aeruginosa and Bacillus subtilis
Protein Engineering 7: 523

Jaeger KE, Ransac S, Koch HB, Ferrato F, Dijkstra BW (1993)
Topological characterization and modeling of the 3D structure of lipase from Pseudomonas aeruginosa
FEBS Letters 332: 143

Jaeger KE, Adrian FJ, Meyer HE, Hancock RE, Winkler UK (1992)
Extracellular lipase from Pseudomonas aeruginosa is an amphiphilic protein
Biochimica & Biophysica Acta 1120: 315

Jaeger KE, Kharazmi A, Hoiby N (1991)
Extracellular lipase of Pseudomonas aeruginosa: biochemical characterization and effect on human neutrophil and monocyte function in vitro
Microb Pathog 10: 173

Kouker G, Jaeger KE (1987)
Specific and sensitive plate assay for bacterial lipases
Applied Environmental Microbiology 53: 211

Stuer W, Jaeger KE, Winkler UK (1986)
Purification of extracellular lipase from Pseudomonas aeruginosa
Journal of Bacteriology 168: 1070