Jones BJ, Evans RL, 3rd, Mylrea NJ, Chaudhury D, Luo C, Guan B, Pierce CT, Gordon WR, Wilmot CM, Kazlauskas RJ (2020)
Larger active site in an ancestral hydroxynitrile lyase increases catalytically promiscuous esterase activity
PLoS ONE 15: e0235341

Jones BJ, Bata Z, Kazlauskas RJ (2017)
Identical active sites in hydroxynitrile lyases show opposite enantioselectivity and reveal possible ancestral mechanism
ACS Catal 7: 4221

Jones BJ, Lim HY, Huang J, Kazlauskas RJ (2017)
Comparison of Five Protein Engineering Strategies for Stabilizing an alpha/beta-Hydrolase
Biochemistry 56: 6521

Devamani T, Rauwerdink AM, Lunzer M, Jones BJ, Mooney JL, Tan MA, Zhang ZJ, Xu JH, Dean AM, Kazlauskas RJ (2016)
Catalytic Promiscuity of Ancestral Esterases and Hydroxynitrile Lyases
Journal of the American Chemical Society 138: 1046

Rauwerdink A, Lunzer M, Devamani T, Jones B, Mooney J, Zhang ZJ, Xu JH, Kazlauskas RJ, Dean AM (2016)
Evolution of a Catalytic Mechanism
Molecular Biology Evolution 33: 971

Huang J, Jones BJ, Kazlauskas RJ (2015)
Stabilization of an alpha/beta-Hydrolase by Introducing Proline Residues: Salicylic Acid Binding Protein 2 from Tobacco
Biochemistry 54: 4330

Rauwerdink A, Kazlauskas RJ (2015)
How the Same Core Catalytic Machinery Catalyzes 17 Different Reactions: the Serine-Histidine-Aspartate Catalytic Triad of alpha/beta-Hydrolase Fold Enzymes
ACS Catal 5: 6153

Nedrud DM, Lin H, Lopez G, Padhi SK, Legatt GA, Kazlauskas RJ (2014)
Uncovering divergent evolution of alpha/beta-hydrolases: a surprising residue substitution needed to convert hydroxynitrile lyase into an esterase
Chem Sci 5: 4265

von Langermann J, Nedrud DM, Kazlauskas RJ (2014)
Increasing the reaction rate of hydroxynitrile lyase from Hevea brasiliensis toward mandelonitrile by copying active site residues from an esterase that accepts aromatic esters
Chembiochem 15: 1931

Yin DT, Purpero VM, Fujii R, Jing Q, Kazlauskas RJ (2013)
New structural motif for carboxylic acid perhydrolases
Chemistry 19: 3037

Bornscheuer UT, Huisman GW, Kazlauskas RJ, Lutz S, Moore JC, Robins K (2012)
Engineering the third wave of biocatalysis
Nature 485: 185

Yin DT, Kazlauskas RJ (2012)
Revised molecular basis of the promiscuous carboxylic acid perhydrolase activity in serine hydrolases
Chemistry 18: 8130

Bornscheuer U, Kazlauskas RJ (2011)
Survey of protein engineering strategies
Curr Protoc Protein Science Chapter 26: Unit26 7

Colton IJ, Yin DT, Grochulski P, Kazlauskas RJ (2011)
Insights into the molecular basis of chiral acid recognition by Candida rugosa lipase from an X-ray crystal structure of a bound phosphonate transition state analog
Adv Synth Catal 353: 2529

Jiang Y, Morley KL, Schrag JD, Kazlauskas RJ (2011)
Different active-site loop orientation in serine hydrolases versus acyltransferases
Chembiochem 12: 768

Jochens H, Hesseler M, Stiba K, Padhi SK, Kazlauskas RJ, Bornscheuer UT (2011)
Protein engineering of alpha/beta-hydrolase fold enzymes
Chembiochem 12: 1508

Yin DT, Jing Q, Aldajani WW, Duncan S, Tschirner U, Schilling J, Kazlauskas RJ (2011)
Improved pretreatment of lignocellulosic biomass using enzymatically-generated peracetic acid
Bioresour Technol 102: 5183

Duncan S, Jing Q, Katona A, Kazlauskas RJ, Schilling J, Tschirner U, Aldajani WW (2010)
Increased saccharification yields from aspen biomass upon treatment with enzymatically generated peracetic acid
Appl Biochem Biotechnol 160: 1637

Padhi SK, Fujii R, Legatt GA, Fossum SL, Berchtold R, Kazlauskas RJ (2010)
Switching from an esterase to a hydroxynitrile lyase mechanism requires only two amino acid substitutions
Chemical Biology 17: 863

Yin DL, Bernhardt P, Morley KL, Jiang Y, Cheeseman JD, Purpero V, Schrag JD, Kazlauskas RJ (2010)
Switching catalysis from hydrolysis to perhydrolysis in Pseudomonas fluorescens esterase
Biochemistry 49: 1931

Jochens H, Stiba K, Savile C, Fujii R, Yu JG, Gerassenkov T, Kazlauskas RJ, Bornscheuer UT (2009)
Converting an esterase into an epoxide hydrolase
Angew Chem Int Ed Engl 48: 3532

Kazlauskas RJ, Bornscheuer UT (2009)
Finding better protein engineering strategies
Nat Chemical Biology 5: 526

Park JH, Ha HJ, Lee WK, Genereux-Vincent T, Kazlauskas RJ (2009)
Molecular basis for the stereoselective ammoniolysis of N-alkyl aziridine-2-carboxylates catalyzed by Candida antarctica lipase B
Chembiochem 10: 2213

Jing Q, Kazlauskas RJ (2008)
Determination of absolute configuration of secondary alcohols using lipase-catalyzed kinetic resolutions
Chirality 20: 724

Gorke JT, Okrasa K, Louwagie A, Kazlauskas RJ, Srienc F (2007)
Enzymatic synthesis of poly(hydroxyalkanoates) in ionic liquids
J Biotechnol 132: 306

Lavandera I, Fernandez S, Magdalena J, Ferrero M, Grewal H, Savile CK, Kazlauskas RJ, Gotor V (2006)
Remote interactions explain the unusual regioselectivity of lipase from Pseudomonas cepacia toward the secondary hydroxyl of 2'-deoxynucleosides
Chembiochem 7: 693

Nisole A, Lussier FX, Morley KL, Shareck F, Kazlauskas RJ, Dupont C, Pelletier JN (2006)
Extracellular production of Streptomyces lividans acetyl xylan esterase A in Escherichia coli for rapid detection of activity
Protein Expr Purif 46: 274

Bernhardt P, Hult K, Kazlauskas RJ (2005)
Molecular basis of perhydrolase activity in serine hydrolases
Angew Chem Int Ed Engl 44: 2742

Kazlauskas RJ (2005)
Enhancing catalytic promiscuity for biocatalysis
Curr Opin Chemical Biology 9: 195

Lavandera I, Fernandez S, Magdalena J, Ferrero M, Kazlauskas RJ, Gotor V (2005)
An inverse substrate orientation for the regioselective acylation of 3',5'-diaminonucleosides catalyzed by Candida antarctica lipase B?
Chembiochem 6: 1381

Mezzetti A, Schrag JD, Cheong CS, Kazlauskas RJ (2005)
Mirror-image packing in enantiomer discrimination molecular basis for the enantioselectivity of B.cepacia lipase toward 2-methyl-3-phenyl-1-propanol
Chemical Biology 12: 427

Park S, Morley KL, Horsman GP, Holmquist M, Hult K, Kazlauskas RJ (2005)
Focusing mutations into the P. fluorescens esterase binding site increases enantioselectivity more effectively than distant mutations
Chemical Biology 12: 45

Cheeseman JD, Tocilj A, Park S, Schrag JD, Kazlauskas RJ (2004)
Structure of an aryl esterase from Pseudomonas fluorescens
Acta Crystallographica D Biol Crystallogr 60: 1237

Horsman GP, Liu AM, Henke E, Bornscheuer UT, Kazlauskas RJ (2003)
Mutations in distant residues moderately increase the enantioselectivity of Pseudomonas fluorescens esterase towards methyl 3bromo-2-methylpropanoate and ethyl 3phenylbutyrate
Chemistry 9: 1933

Gascoyne DG, Finkbeiner HL, Chan KP, Gordon JL, Stewart KR, Kazlauskas RJ (2001)
Molecular basis for enantioselectivity of lipase from Chromobacterium viscosum toward the diesters of 2,3-dihydro-3-(4'-hydroxyphenyl)-1,1,3-trimethyl-1H-inden-5-ol
J Org Chem 66: 3041

Tuomi WV, Kazlauskas RJ (1999)
Molecular Basis for Enantioselectivity of Lipase from Pseudomonas cepacia toward Primary Alcohols. Modeling, Kinetics, and Chemical Modification of Tyr29 to Increase or Decrease Enantioselectivity
J Org Chem 64: 2638

Cygler M, Grochulski P, Kazlauskas RJ, Schrag JD, Bouthillier F, Rubin B, Serreqi AN, Gupta AK (1994)
A structural basis for the chiral preferences of lipases
Journal of the American Chemical Society 116: 3180

Grochulski P, Bouthillier F, Kazlauskas RJ, Serreqi AN, Schrag JD, Ziomek E, Cygler M (1994)
Analogs of reaction intermediates identify a unique substrate binding site in Candida rugosa lipase
Biochemistry 33: 3494

Kazlauskas RJ (1994)
Elucidating structure-mechanism relationships in lipases: prospects for predicting and engineering catalytic properties
Trends Biotechnol 12: 464

Kazlauskas RJ (1993)
Biocatalysis--becoming more predictable and selective
Trends Biotechnol 11: 439