De Luca V, Mandrich L, Manco G (2023) <br />Development of a Qualitative Test to Detect the Presence of Organophosphate Pesticides on Fruits and Vegetables<br /><i>Life</i> <b>13</b>: 490<br /><br />De Luca V, Mandrich L, Manco G (2023) <br />Development of a Qualitative Test to Detect the Presence of Organophosphate Pesticides on Fruits and Vegetables<br /><i>Life (Basel)</i> <b>13</b>: <br /><br />Marone M, Porzio E, Lampitella EA, Manco G (2023) <br />A mesophilic phosphotriesterase-like lactonase shows high stability and proficiency as quorum quenching enzyme<br /><i>Chemico-Biological Interactions</i> <b>383</b>: 110657<br /><br />Garefalaki V, Manco G, Porzio E (2022) <br />Use of biosensors for rapid and sensitive detection of pesticides in food samples for food safety chemical risk assessment<br /><i>EFSA J</i> <b>20</b>: e200922<br /><br />Carullo P, Chino M, Cetrangolo GP, Terreri S, Lombardi A, Manco G, Cimmino A, Febbraio F (2018) <br />Direct detection of organophosphate compounds in water by a fluorescence-based biosensing device<br /><i>Sensors and Actuators B: Chemical</i> <b>255</b>: 3257<br /><br />Manco G, Porzio E, Suzumoto Y (2018) <br />Enzymatic detoxication: a sustainable means of degrading toxic organophosphate pesticides and chemical warfare nerve agents<br /><i>J Chem Technol Biotechnol</i> <b>93</b>: 2064<br /><br />Porzio E, Bettazzi F, Mandrich L, Del Giudice I, Restaino OF, Laschi S, Febbraio F, De Luca V, Borzacchiello MG, Carusone TM, Worek F, Pisanti A, Porcaro P, Schiraldi C, De Rosa M, Palchetti I, Manco G (2018) <br />Innovative Biocatalysts as Tools to Detect and Inactivate Nerve Agents<br /><i>Sci Rep</i> <b>8</b>: 13773<br /><br />Restaino OF, Borzacchiello MG, Scognamiglio I, Fedele L, Alfano A, Porzio E, Manco G, De Rosa M, Schiraldi C (2018) <br />High yield production and purification of two recombinant thermostable phosphotriesterase-like lactonases from Sulfolobus acidocaldarius and Sulfolobus solfataricus useful as bioremediation tools and bioscavengers<br /><i>BMC Biotechnol</i> <b>18</b>: 18<br /><br />Del Giudice I, Coppolecchia R, Merone L, Porzio E, Carusone TM, Mandrich L, Worek F, Manco G (2016) <br />An efficient thermostable organophosphate hydrolase and its application in pesticide decontamination<br /><i>Biotechnol Bioeng</i> <b>113</b>: 724<br /><br />Pennacchio A, Mandrich L, Manco G, Trincone A (2015) <br />Enlarging the substrate portfolio of the thermophilic esterase EST2 from Alicyclobacillus acidocaldarius<br /><i>Extremophiles</i> <b>19</b>: 1001<br /><br />Tang X, Liang B, Yi T, Manco G, Ilariapalchetti, Liu A (2014) <br />Cell surface display of organophosphorus hydrolase for sensitive spectrophotometric detection of p-nitrophenol substituted organophosphates<br /><i>Enzyme Microb Technol</i> <b>55</b>: 107<br /><br />Mandrich L, Di Gennaro S, Palma A, Manco G (2013) <br />A Further Biochemical Characterization of DrPLL the Thermophilic Lactonase from Deinococcus radiodurans<br /><i>Protein Pept Lett</i> <b>20</b>: 36<br /><br />Pezzullo M, Del Vecchio P, Mandrich L, Nucci R, Rossi M, Manco G (2013) <br />Comprehensive analysis of surface charged residues involved in thermal stability in Alicyclobacillus acidocaldarius esterase 2<br /><i>Protein Engineering Des Sel</i> <b>26</b>: 47<br /><br />Porzio E, Di Gennaro S, Palma A, Manco G (2013) <br />Mn(2+) modulates the kinetic properties of an archaeal member of the PLL family<br /><i>Chemico-Biological Interactions</i> <b>203</b>: 251<br /><br />Manco G, Merone L, Porzio E, Feng Y, Mandrich L (2012) <br />Enzyme Promiscuity in the Hormone-Sensitive Lipase Family of Proteins<br /><i>Protein Pept Lett</i> <b>19</b>: 144<br /><br />Chahinian H, Fantini J, Garmy N, Manco G, Sarda L (2010) <br />Non-lipolytic and lipolytic sequence-related carboxylesterases: a comparative study of the structure-function relationships of rabbit liver esterase 1 and bovine pancreatic bile-salt-activated lipase<br /><i>Biochimica & Biophysica Acta</i> <b>1801</b>: 1195<br /><br />Mandrich L, Merone L, Manco G (2010) <br />Hyperthermophilic phosphotriesterases/lactonases for the environment and human health<br /><i>Environ Technol</i> <b>31</b>: 1115<br /><br />Merone L, Mandrich L, Porzio E, Rossi M, Muller S, Reiter G, Worek F, Manco G (2010) <br />Improving the promiscuous nerve agent hydrolase activity of a thermostable archaeal lactonase<br /><i>Bioresour Technol</i> <b>101</b>: 9204<br /><br />Manco G, Nucci R, Febbraio F (2009) <br />Use of esterase activities for the detection of chemical neurotoxic agents<br /><i>Protein Pept Lett</i> <b>16</b>: 1225<br /><br />Mandrich L, Manco G (2009) <br />Evolution in the amidohydrolase superfamily: substrate-assisted gain of function in the E183K mutant of a phosphotriesterase-like metal-carboxylesterase<br /><i>Biochemistry</i> <b>48</b>: 5602<br /><br />Mandrich L, Merone L, Manco G (2009) <br />Structural and kinetic overview of the carboxylesterase EST2 from alicyclobacillus acidocaldarius: a comparison with the other members of the HSL family<br /><i>Protein Pept Lett</i> <b>16</b>: 1189<br /><br />Elias M, Dupuy J, Merone L, Mandrich L, Porzio E, Moniot S, Rochu D, Lecomte C, Rossi M, Masson P, Manco G, Chabriere E (2008) <br />Structural basis for natural lactonase and promiscuous phosphotriesterase activities<br /><i>Journal of Molecular Biology</i> <b>379</b>: 1017<br /><br />Febbraio F, D'Andrea SE, Mandrich L, Merone L, Rossi M, Nucci R, Manco G (2008) <br />Irreversible inhibition of the thermophilic esterase EST2 from Alicyclobacillus acidocaldarius<br /><i>Extremophiles</i> <b>12</b>: 719<br /><br />Mandrich L, Menchise V, Alterio V, De Simone G, Pedone C, Rossi M, Manco G (2008) <br />Functional and structural features of the oxyanion hole in a thermophilic esterase from Alicyclobacillus acidocaldarius<br /><i>Proteins</i> <b>71</b>: 1721<br /><br />Mandrich L, Pezzullo M, Rossi M, Manco G (2007) <br />SSoNDelta and SSoNDeltalong: two thermostable esterases from the same ORF in the archaeon Sulfolobus solfataricus?<br /><i>Archaea</i> <b>2</b>: 109<br /><br />Porzio E, Merone L, Mandrich L, Rossi M, Manco G (2007) <br />A new phosphotriesterase from Sulfolobus acidocaldarius and its comparison with the homologue from Sulfolobus solfataricus<br /><i>Biochimie</i> <b>89</b>: 625<br /><br />Afriat L, Roodveldt C, Manco G, Tawfik DS (2006) <br />The latent promiscuity of newly identified microbial lactonases is linked to a recently diverged phosphotriesterase<br /><i>Biochemistry</i> <b>45</b>: 13677<br /><br />Ben Ali Y, Chahinian H, Petry S, Muller G, Lebrun R, Verger R, Carriere F, Mandrich L, Rossi M, Manco G, Sarda L, Abousalham A (2006) <br />Use of an inhibitor to identify members of the hormone-sensitive lipase family<br /><i>Biochemistry</i> <b>45</b>: 14183<br /><br />Mandrich L, Manco G, Rossi M, Floris E, Jansen-van den Bosch T, Smit G, Wouters JA (2006) <br />Alicyclobacillus acidocaldarius thermophilic esterase EST2's activity in milk and cheese models<br /><i>Applied Environmental Microbiology</i> <b>72</b>: 3191<br /><br />Chahinian H, Ben Ali Y, Abousalham A, Petry S, Mandrich L, Manco G, Canaan S, Sarda L (2005) <br />Substrate specificity and kinetic properties of enzymes belonging to the hormone-sensitive lipase family: comparison with non-lipolytic and lipolytic carboxylesterases<br /><i>Biochimica & Biophysica Acta</i> <b>1738</b>: 29<br /><br />Mandrich L, Merone L, Pezzullo M, Cipolla L, Nicotra F, Rossi M, Manco G (2005) <br />Role of the N terminus in enzyme activity, stability and specificity in thermophilic esterases belonging to the HSL family<br /><i>Journal of Molecular Biology</i> <b>345</b>: 501<br /><br />De Simone G, Mandrich L, Menchise V, Giordano V, Febbraio F, Rossi M, Pedone C, Manco G (2004) <br />A substrate-induced switch in the reaction mechanism of a thermophilic esterase: kinetic evidences and structural basis<br /><i>Journal of Biological Chemistry</i> <b>279</b>: 6815<br /><br />De Simone G, Menchise V, Alterio V, Mandrich L, Rossi M, Manco G, Pedone C (2004) <br />The crystal structure of an EST2 mutant unveils structural insights on the H group of the carboxylesterase/lipase family<br /><i>Journal of Molecular Biology</i> <b>343</b>: 137<br /><br />Mandrich L, Pezzullo M, Del Vecchio P, Barone G, Rossi M, Manco G (2004) <br />Analysis of thermal adaptation in the HSL enzyme family<br /><i>Journal of Molecular Biology</i> <b>335</b>: 357<br /><br />Del Vecchio P, Graziano G, Granata V, Barone G, Mandrich L, Rossi M, Manco G (2003) <br />Effect of trifluoroethanol on the conformational stability of a hyperthermophilic esterase: a CD study<br /><i>Biophysical Chemistry</i> <b>104</b>: 407<br /><br />Sorrentino N, De Simone G, Menchise V, Mandrich L, Rossi M, Manco G, Pedone C (2003) <br />Crystallization and preliminary X-ray diffraction studies of Aes acetyl-esterase from Escherichia coli<br /><i>Acta Crystallographica D Biol Crystallogr</i> <b>59</b>: 1846<br /><br />Mandrich L, Caputo E, Martin BM, Rossi M, Manco G (2002) <br />The Aes protein and the monomeric alpha-galactosidase from Escherichia coli form a non-covalent complex. Implications for the regulation of carbohydrate metabolism<br /><i>Journal of Biological Chemistry</i> <b>277</b>: 48241<br /><br />De Simone G, Menchise V, Manco G, Mandrich L, Sorrentino N, Lang D, Rossi M, Pedone C (2001) <br />The crystal structure of a hyper-thermophilic carboxylesterase from the archaeon Archaeoglobus fulgidus<br /><i>Journal of Molecular Biology</i> <b>314</b>: 507<br /><br />Manco G, Mandrich L, Rossi M (2001) <br />Residues at the active site of the esterase 2 from Alicyclobacillus acidocaldarius involved in substrate specificity and catalytic activity at high temperature<br /><i>Journal of Biological Chemistry</i> <b>276</b>: 37482<br /><br />D'Auria S, Herman P, Lakowicz JR, Tanfani F, Bertoli E, Manco G, Rossi M (2000) <br />The esterase from the thermophilic eubacterium Bacillus acidocaldarius: structural-functional relationship and comparison with the esterase from the hyperthermophilic archaeon Archaeoglobus fulgidus<br /><i>Proteins</i> <b>40</b>: 473<br /><br />De Simone G, Galdiero S, Manco G, Lang D, Rossi M, Pedone C (2000) <br />A snapshot of a transition state analogue of a novel thermophilic esterase belonging to the subfamily of mammalian hormone-sensitive lipase<br /><i>Journal of Molecular Biology</i> <b>303</b>: 761<br /><br />Manco G, Camardella L, Febbraio F, Adamo G, Carratore V, Rossi M (2000) <br />Homology modeling and identification of serine 160 as nucleophile of the active site in a thermostable carboxylesterase from the archaeon Archaeoglobus fulgidus<br /><i>Protein Engineering</i> <b>13</b>: 197<br /><br />Manco G, Giosue E, D'Auria S, Herman P, Carrea G, Rossi M (2000) <br />Cloning, overexpression, and properties of a new thermophilic and thermostable esterase with sequence similarity to hormone-sensitive lipase subfamily from the archaeon Archaeoglobus fulgidus<br /><i>Archives of Biochemistry & Biophysics</i> <b>373</b>: 182<br /><br />De Simone G, Manco G, Galdiero S, Lombardi A, Rossi M, Pavone V (1999) <br />Crystallization and preliminary X-ray diffraction studies of the carboxylesterase EST2 from Alicyclobacillus acidocaldarius<br /><i>Acta Crystallographica D Biol Crystallogr</i> <b>55</b>: 1348<br /><br />Manco G, Febbraio F, Adinolfi E, Rossi M (1999) <br />Homology modeling and active-site residues probing of the thermophilic Alicyclobacillus acidocaldarius esterase 2<br /><i>Protein Science</i> <b>8</b>: 1789<br /><br />Manco G, Adinolfi E, Pisani FM, Ottolina G, Carrea G, Rossi M (1998) <br />Overexpression and properties of a new thermophilic and thermostable esterase from Bacillus acidocaldarius with sequence similarity to hormone-sensitive lipase subfamily<br /><i>Biochemical Journal</i> <b>332 ( Pt 1)</b>: 203<br /><br />