Li D, Wu Q, Reetz MT (2020)
Focused rational iterative site-specific mutagenesis (FRISM)
Methods Enzymol 643: 225

Cen Y, Singh W, Arkin M, Moody TS, Huang M, Zhou J, Wu Q, Reetz MT (2019)
Artificial cysteine-lipases with high activity and altered catalytic mechanism created by laboratory evolution
Nat Commun 10: 3198

Reetz MT (2019)
Directed Evolution of Artificial Metalloenzymes: A Universal Means to Tune the Selectivity of Transition Metal Catalysts?
Acc Chem Res 52: 336

Xu J, Cen Y, Singh W, Fan J, Wu L, Lin X, Zhou J, Huang M, Reetz MT, Wu Q (2019)
Stereodivergent Protein Engineering of a Lipase To Access All Possible Stereoisomers of Chiral Esters with Two Stereocenters
Journal of the American Chemical Society 141: 7934

Augustyniak W, Wienk H, Boelens R, Reetz MT (2013)
(1)H, (13)C and (15)N resonance assignments of wild-type Bacillus subtilis Lipase A and its mutant evolved towards thermostability
Biomol NMR Assign 7: 249

Augustyniak W, Brzezinska AA, Pijning T, Wienk H, Boelens R, Dijkstra BW, Reetz MT (2012)
Biophysical characterization of mutants of Bacillus subtilis lipase evolved for thermostability: factors contributing to increased activity retention
Protein Science 21: 487

Feng X, Sanchis J, Reetz MT, Rabitz H (2012)
Enhancing the efficiency of directed evolution in focused enzyme libraries by the adaptive substituent reordering algorithm
Chemistry 18: 5646

Prasad S, Bocola M, Reetz MT (2011)
Revisiting the lipase from Pseudomonas aeruginosa: directed evolution of substrate acceptance and enantioselectivity using iterative saturation mutagenesis
Chemphyschem 12: 1550

Reetz MT, Zheng H (2011)
Manipulating the expression rate and enantioselectivity of an epoxide hydrolase by using directed evolution
Chembiochem 12: 1529

Reetz MT, Soni P, Fernandez L, Gumulya Y, Carballeira JD (2010)
Increasing the stability of an enzyme toward hostile organic solvents by directed evolution based on iterative saturation mutagenesis using the B-FIT method
Chem Commun (Camb) 46: 8657

Zheng H, Reetz MT (2010)
Manipulating the stereoselectivity of limonene epoxide hydrolase by directed evolution based on iterative saturation mutagenesis
Journal of the American Chemical Society 132: 15744

Reetz MT, Soni P, Fernandez L (2009)
Knowledge-guided laboratory evolution of protein thermolability
Biotechnol Bioeng 102: 1712

Reetz MT, Bocola M, Wang LW, Sanchis J, Cronin A, Arand M, Zou J, Archelas A, Bottalla AL, Naworyta A, Mowbray SL (2009)
Directed evolution of an enantioselective epoxide hydrolase: uncovering the source of enantioselectivity at each evolutionary stage
Journal of the American Chemical Society 131: 7334

Reetz MT, Hobenreich H, Soni P, Fernandez L (2008)
A genetic selection system for evolving enantioselectivity of enzymes
Chem Commun (Camb)

Sanchis J, Fernandez L, Carballeira JD, Drone J, Gumulya Y, Hobenreich H, Kahakeaw D, Kille S, Lohmer R, Peyralans JJ, Podtetenieff J, Prasad S, Soni P, Taglieber A, Wu S, Zilly FE, Reetz MT (2008)
Improved PCR method for the creation of saturation mutagenesis libraries in directed evolution: application to difficult-to-amplify templates
Applied Microbiology & Biotechnology 81: 387

Reetz MT, Puls M, Carballeira JD, Vogel A, Jaeger KE, Eggert T, Thiel W, Bocola M, Otte N (2007)
Learning from directed evolution: Further lessons from theoretical investigations into cooperative mutations in lipase enantioselectivity
Chembiochem 8: 106

Droge MJ, Boersma YL, van Pouderoyen G, Vrenken TE, Ruggeberg CJ, Reetz MT, Dijkstra BW, Quax WJ (2006)
Directed evolution of Bacillus subtilis lipase A by use of enantiomeric phosphonate inhibitors: crystal structures and phage display selection
Chembiochem 7: 149

Reetz MT, Carballeira JD, Peyralans J, Hobenreich H, Maichele A, Vogel A (2006)
Expanding the substrate scope of enzymes: combining mutations obtained by CASTing
Chemistry 12: 6031

Eggert T, Funke SA, Rao NM, Acharya P, Krumm H, Reetz MT, Jaeger KE (2005)
Multiplex-PCR-based recombination as a novel high-fidelity method for directed evolution
Chembiochem 6: 1062

Bocola M, Otte N, Jaeger KE, Reetz MT, Thiel W (2004)
Learning from directed evolution: theoretical investigations into cooperative mutations in lipase enantioselectivity
Chembiochem 5: 214

Reetz MT (2004)
Controlling the enantioselectivity of enzymes by directed evolution: practical and theoretical ramifications
Proc Natl Acad Sci U S A 101: 5716

Reetz MT, Torre C, Eipper A, Lohmer R, Hermes M, Brunner B, Maichele A, Bocola M, Arand M, Cronin A, Genzel Y, Archelas A, Furstoss R (2004)
Enhancing the enantioselectivity of an epoxide hydrolase by directed evolution
Org Lett 6: 177

Reetz MT, Wiesenhofer W (2004)
Liquid poly(ethylene glycol) and supercritical carbon dioxide as a biphasic solvent system for lipase-catalyzed esterification
Chem Commun (Camb)

Droge MJ, Ruggeberg CJ, van der Sloot AM, Schimmel J, Dijkstra DS, Verhaert RM, Reetz MT, Quax WJ (2003)
Binding of phage displayed Bacillus subtilis lipase A to a phosphonate suicide inhibitor
J Biotechnol 101: 19

Reetz MT (2002)
Lipases as practical biocatalysts
Curr Opin Chemical Biology 6: 145

Jaeger KE, Eggert T, Eipper A, Reetz MT (2001)
Directed evolution and the creation of enantioselective biocatalysts
Applied Microbiology & Biotechnology 55: 519

Reetz MT, Wilensek S, Zha D, Jaeger KE (2001)
Directed Evolution of an Enantioselective Enzyme through Combinatorial Multiple-Cassette Mutagenesis
Angew Chem Int Ed Engl 40: 3589

Jaeger KE, Reetz MT (2000)
Directed evolution of enantioselective enzymes for organic chemistry
Curr Opin Chemical Biology 4: 68

Liebeton K, Zonta A, Schimossek K, Nardini M, Lang D, Dijkstra BW, Reetz MT, Jaeger KE (2000)
Directed evolution of an enantioselective lipase
Chemical Biology 7: 709

Reetz MT, Jaeger KE (2000)
Enantioselective enzymes for organic synthesis created by directed evolution
Chemistry 6: 407

Jaeger KE, Dijkstra BW, Reetz MT (1999)
Bacterial biocatalysts: molecular biology, three-dimensional structures, and biotechnological applications of lipases
Annu Rev Microbiol 53: 315

Jaeger KE, Reetz MT (1998)
Microbial lipases form versatile tools for biotechnology
Trends Biotechnol 16: 396

Reetz MT, Jaeger KE (1998)
Overexpression, immobilization and biotechnological application of Pseudomonas lipases
Chemistry & Physic of Lipids 93: 3