Kronman C, Cohen O, Mazor O, Ordentlich A, Raveh L, Velan B, Shafferman A (2010) <br />Next generation OP-bioscavengers: a circulatory long-lived 4-PEG hypolysine mutant of F338A-HuAChE with optimal pharmacokinetics and pseudo-catalytic characteristics<br /><i>Chemico-Biological Interactions</i> <b>187</b>: 253<br /><br />Lustig S, Maik-Rachline G, Paran N, Melamed S, Israely T, Erez N, Orr N, Reuveny S, Ordentlich A, Laub O, Shafferman A, Velan B (2009) <br />Effective post-exposure protection against lethal orthopoxviruses infection by vaccinia immune globulin involves induction of adaptive immune response<br /><i>Vaccine</i> <b>27</b>: 1691<br /><br />Paran N, Suezer Y, Lustig S, Israely T, Schwantes A, Melamed S, Katz L, Preuss T, Hanschmann KM, Kalinke U, Erez N, Levin R, Velan B, Lower J, Shafferman A, Sutter G (2009) <br />Postexposure immunization with modified vaccinia virus Ankara or conventional Lister vaccine provides solid protection in a murine model of human smallpox<br /><i>J Infect Dis</i> <b>199</b>: 39<br /><br />Shafferman A, Barak D, Stein D, Kronman C, Velan B, Greig NH, Ordentlich A (2008) <br />Flexibility versus rigidity of the functional architecture of AChE active center<br /><i>Chemico-Biological Interactions</i> <b>175</b>: 166<br /><br />Cohen O, Kronman C, Lazar A, Velan B, Shafferman A (2007) <br />Controlled concealment of exposed clearance and immunogenic domains by site-specific polyethylene glycol attachment to acetylcholinesterase hypolysine mutants<br /><i>Journal of Biological Chemistry</i> <b>282</b>: 35491<br /><br />Barak D, Ordentlich A, Kaplan D, Kronman C, Velan B, Shafferman A (2005) <br />Lessons from functional analysis of AChE covalent and noncovalent inhibitors for design of AD therapeutic agents<br /><i>Chemico-Biological Interactions</i> <b>157-158</b>: 219<br /><br />Kronman C, Cohen O, Velan B, Shafferman A (2005) <br />Host-regulated disposition of mammalian AChEs<br /><i>Chemico-Biological Interactions</i> <b>157-158</b>: 51<br /><br />Ordentlich A, Barak D, Sod-Moriah G, Kaplan D, Mizrahi D, Segall Y, Kronman C, Karton Y, Lazar A, Marcus D, Velan B, Shafferman A (2005) <br />The role of AChE active site gorge in determining stereoselectivity of charged and noncharged VX enantiomers<br /><i>Chemico-Biological Interactions</i> <b>157-158</b>: 191<br /><br />Shafferman A, Barak D, Kaplan D, Ordentlich A, Kronman C, Velan B (2005) <br />Functional requirements for the optimal catalytic configuration of the AChE active center<br /><i>Chemico-Biological Interactions</i> <b>157-158</b>: 123<br /><br />Barak D, Ordentlich A, Kaplan D, Elhanani E, Segall Y, Barak R, Velan B, Shafferman A (2004) <br />Poster (23) Phosphorylation and aging of AChE - insights from mutagenesis, mass spectrometry and structural studies<br /><i>In: Cholinesterases in the Second Millennium: Biomolecular and Pathological Aspects</i> <b> (Inestrosa NC, Campos EO) P. Universidad Catolica de Chile-FONDAP Biomedicina</b>: 332<br /><br />Chitlaru T, Kronman C, Lazar S, Seliger N, Velan B, Shafferman A (2004) <br />Effect of post-translation modifications of human acetylcholinesterase on its circulatory residence.<br /><i>Cholinergic Mechanisms, CRC Press</i> <br /><br />Cohen O, Kronman C, Velan B, Shafferman A (2004) <br />Amino acid domains control the circulatory residence time of primate acetylcholinesterases in rhesus macaques (Macaca mulatta)<br /><i>Biochemical Journal</i> <b>378</b>: 117<br /><br />Cohen O, Kronman C, Chitlaru T, Ordentlich A, Velan B, Shafferman A (2004) <br />Generation of pharmacokinetically improved recombinant human acetylcholinesterase by polyethylene glycol modification.<br /><i>Cholinergic Mechanisms, CRC Press</i> <br /><br />Elhanany E, Ordentlich A, Dgany OR, Kaplan D, Segall Y, Barak R, Velan B, Shafferman A (2004) <br />MALDI-TOF/MS analysis of tabun-acetylcholinesterase conjugate.<br /><i>Cholinergic Mechanisms, CRC Press</i> <br /><br />Kaplan D, Barak D, Ordentlich A, Kronman C, Velan B, Shafferman A (2004) <br />Is aromaticity essential for trapping the catalytic histidine 447 in human acetylcholinesterase?<br /><i>Biochemistry</i> <b>43</b>: 3129<br /><br />Kronman C, Chitlaru T, Seliger N, Lazar S, Lazar A, Zilberstein L, Velan B, Shafferman A (2004) <br />Some basic rules governing oligosaccharide-dependent circulatory residence of glycoproteins are revealed by MALDI-TOF mapping of the multiple N-glycans associated with recombinant bovine acetylcholinesterase.<br /><i>Cholinergic Mechanisms, CRC Press</i> <br /><br />Ordentlich A, Barak D, Ariel N, Kronman C, Kaplan D, Velan B, Shafferman A (2004) <br />Surprising findings from the functional analysis of human acetylcholinesterase adducts of Alzheimer?s disease drugs.<br /><i>Cholinergic Mechanisms, CRC Press</i> <br /><br />Ordentlich A, Kronman C, Barak D, Ariel N, Kaplan D, Velan B, Shafferman A (2004) <br />Poster (21) Analysis of acetylcholinesterase adducts of alzheimer's drugs<br /><i>In: Cholinesterases in the Second Millennium: Biomolecular and Pathological Aspects</i> <b> (Inestrosa NC, Campos EO) P. Universidad Catolica de Chile-FONDAP Biomedicina</b>: 332<br /><br />Ordentlich A, Barak D, Sod-Moriah G, Kaplan D, Mizrahi D, Segall Y, Kronman C, Karton Y, Lazar A, Marcus D, Velan B, Shafferman A (2004) <br />Stereoselectivity toward VX is determined by interactions with residues of the acyl pocket as well as of the peripheral anionic site of AChE<br /><i>Biochemistry</i> <b>43</b>: 11255<br /><br />Shafferman A, Chitlaru T, Ordentlich A, Velan B, Kronman C (2004) <br />A complex array of post-translation modifications determines the circulatory longevity of acetylcholinesterase in a hierarchical manner.<br /><i>Cholinergic Mechanisms, CRC Press</i> <br /><br />Shafferman A, Barak D, Kaplan D, Ordentlich A, Ariel N, Velan B (2004) <br />The aromatic trapping of histidine 447 in catalysis of acetylcholinesterases<br /><i>In: Cholinesterases in the Second Millennium: Biomolecular and Pathological Aspects</i> <b> (Inestrosa NC, Campos EO) P. Universidad Catolica de Chile-FONDAP Biomedicina</b>: 181 <br /><br />Shafferman A, Barak D, Kaplan D, Ordentlich A, Ariel N, Velan B (2004) <br />Poster (14) The aromatic trapping of histidine 447 in catalysis of acetylcholinesterases<br /><i>In: Cholinesterases in the Second Millennium: Biomolecular and Pathological Aspects</i> <b> (Inestrosa NC, Campos EO) P. Universidad Catolica de Chile-FONDAP Biomedicina</b>: 327<br /><br />Velan B, Kronman C, Chitlaru T, Cohen O, Ordentlich A, Shafferman A (2004) <br />Poster (35) The hierarchy of post-translation modifications determining circulatory longevity of acetylcholinesterase.<br /><i>In: Cholinesterases in the Second Millennium: Biomolecular and Pathological Aspects</i> <b> (Inestrosa NC, Campos EO) P. Universidad Catolica de Chile-FONDAP Biomedicina</b>: 339<br /><br />Barak D, Kaplan D, Ordentlich A, Ariel N, Velan B, Shafferman A (2002) <br />The aromatic trapping of the catalytic histidine is essential for efficient catalysis in acetylcholinesterase<br /><i>Biochemistry</i> <b>41</b>: 8245<br /><br />Chitlaru T, Kronman C, Velan B, Shafferman A (2002) <br />Overloading and removal of N-glycosylation targets on human acetylcholinesterase: effects on glycan composition and circulatory residence time<br /><i>Biochemical Journal</i> <b>363</b>: 619<br /><br />Chitlaru T, Kronman C, Velan B, Shafferman A (2001) <br />Effect of human acetylcholinesterase subunit assembly on its circulatory residence<br /><i>Biochemical Journal</i> <b>354</b>: 613<br /><br />Cohen O, Kronman C, Chitlaru T, Ordentlich A, Velan B, Shafferman A (2001) <br />Effect of chemical modification of recombinant human acetylcholinesterase by polyethylene glycol on its circulatory longevity<br /><i>Biochemical Journal</i> <b>357</b>: 795<br /><br />Elhanany E, Ordentlich A, Dgany O, Kaplan D, Segall Y, Barak R, Velan B, Shafferman A (2001) <br />Resolving pathways of interaction of covalent inhibitors with the active site of acetylcholinesterases: maldi-tof/ms analysis of various nerve agent phosphyl adducts<br /><i>Chemical Research in Toxicology</i> <b>14</b>: 912<br /><br />Kaplan D, Ordentlich A, Barak D, Ariel N, Kronman C, Velan B, Shafferman A (2001) <br />Does butyrylization of acetylcholinesterase through substitution of the six divergent aromatic amino acids in the active center gorge generate an enzyme mimic of butyrylcholinesterase?<br /><i>Biochemistry</i> <b>40</b>: 7433<br /><br />Barak D, Ordentlich A, Kaplan D, Barak R, Mizrahi D, Kronman C, Segall Y, Velan B, Shafferman A (2000) <br />Evidence for P-N bond scission in phosphoroamidate nerve agent adducts of human acetylcholinesterase<br /><i>Biochemistry</i> <b>39</b>: 1156<br /><br />Cohen S, Mendelson I, Altboum Z, Kobiler D, Elhanany E, Bino T, Leitner M, Inbar I, Rosenberg H, Gozes Y, Barak R, Fisher M, Kronman C, Velan B, Shafferman A (2000) <br />Attenuated nontoxinogenic and nonencapsulated recombinant Bacillus anthracis spore vaccines protect against anthrax<br /><i>Infect Immun</i> <b>68</b>: 4549<br /><br />Kronman C, Chitlaru T, Elhanany E, Velan B, Shafferman A (2000) <br />Hierarchy of post-translational modifications involved in the circulatory longevity of glycoproteins. Demonstration of concerted contributions of glycan sialylation and subunit assembly to the pharmacokinetic behavior of bovine acetylcholinesterase<br /><i>Journal of Biological Chemistry</i> <b>275</b>: 29488<br /><br />Kryger G, Harel M, Giles K, Toker L, Velan B, Lazar A, Kronman C, Barak D, Ariel N, Shafferman A, Silman I, Sussman JL (2000) <br />Structures of recombinant native and E202Q mutant human acetylcholinesterase complexed with the snake-venom toxin fasciculin-II<br /><i>Acta Crystallographica D Biol Crystallogr</i> <b>56</b>: 1385<br /><br />Malany S, Baker NA, Verweyst M, Medhekar R, Quinn DM, Velan B, Kronman C, Shafferman A (1999) <br />Theoretical and experimental investigations of electrostatic effects on acetylcholinesterase catalysis and inhibition<br /><i>Chemico-Biological Interactions</i> <b>119-120</b>: 99<br /><br />Ordentlich A, Barak D, Kronman C, Benschop HP, De Jong LP, Ariel N, Barak R, Segall Y, Velan B, Shafferman A (1999) <br />Exploring the active center of human acetylcholinesterase with stereomers of an organophosphorus inhibitor with two chiral centers<br /><i>Biochemistry</i> <b>38</b>: 3055<br /><br />Ariel N, Ordentlich A, Barak D, Bino T, Velan B, Shafferman A (1998) <br />Compatibility of Structures Inferred from Mutagenesis and from X-Ray Crystallography for Various AChE Complexes<br /><i>In: Structure and Function of Cholinesterases and Related Proteins - Proceedings of Sixth International Meeting on Cholinesterases</i> <b>(Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. Eds) Plenum Publishing Corp.</b>: 375<br /><br />Ariel N, Velan B, Barak D, Leitner M, Bino T, Ordentlich A, Shafferman A (1998) <br />Structural Modifications of the Omega Loop in Human Acetylcholinesterase<br /><i>In: Structure and Function of Cholinesterases and Related Proteins - Proceedings of Sixth International Meeting on Cholinesterases</i> <b>(Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. Eds) Plenum Publishing Corp.</b>: 453<br /><br />Ariel N, Ordentlich A, Barak D, Bino T, Velan B, Shafferman A (1998) <br />The 'aromatic patch' of three proximal residues in the human acetylcholinesterase active centre allows for versatile interaction modes with inhibitors<br /><i>Biochemical Journal</i> <b>335</b>: 95<br /><br />Barak D, Ordentlich A, Stein D, Segall Y, Velan B, Benschop HP, De Jong LP, Shafferman A (1998) <br />The Aromatic Moiety at Position-86 of HuAChE Accelerate the Aging of Phosphonyl-AChE Conjugates through Cation- Interactions<br /><i>In: Structure and Function of Cholinesterases and Related Proteins - Proceedings of Sixth International Meeting on Cholinesterases</i> <b>(Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. Eds) Plenum Publishing Corp.</b>: 246<br /><br />Chitlaru T, Kronman C, Zeevi M, Kam M, Harel A, Ordentlich A, Velan B, Shafferman A (1998) <br />Modulation of circulatory residence of recombinant acetylcholinesterase through biochemical or genetic manipulation of sialylation levels<br /><i>Biochemical Journal</i> <b>336</b>: 647<br /><br />Giles K, Ben-Yohanan R, Velan B, Shafferman A, Sussman JL, Silman I (1998) <br />Assembly of Acetylcholinesterase Subunits in vitro<br /><i>In: Structure and Function of Cholinesterases and Related Proteins - Proceedings of Sixth International Meeting on Cholinesterases</i> <b>(Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. Eds) Plenum Publishing Corp.</b>: 442<br /><br />Kryger G, Giles K, Harel M, Toker L, Velan B, Lazar A, Kronman C, Barak D, Ariel N, Shafferman A, Silman I, Sussman JL (1998) <br />3D Structure at 2.7  Resolution of Native and E202Q Mutant Human Acetylcholinesterase Complexed with Fasciculin-II<br /><i>In: Structure and Function of Cholinesterases and Related Proteins - Proceedings of Sixth International Meeting on Cholinesterases</i> <b>(Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. Eds) Plenum Publishing Corp.</b>: 323<br /><br />Kryger G, Giles K, Harel M, Toker L, Velan B, Lazar A, Kronman C, Barak D, Ariel N, Shafferman A, Silman I, Sussman JL (1998) <br />3D Structure of a Complex of Human Acetylcholinesterase with Fasciculin-II at 2.7  Resolution<br /><i>In: Structure and Function of Cholinesterases and Related Proteins - Proceedings of Sixth International Meeting on Cholinesterases</i> <b>(Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. Eds) Plenum Publishing Corp.</b>: 370<br /><br />Malany S, Taylor P, Quinn DM, Sawai M, Shafferman A, Velan B, Kronman C (1998) <br />Correlation of Isotope and Viscosity Effects<br /><i>In: Structure and Function of Cholinesterases and Related Proteins - Proceedings of Sixth International Meeting on Cholinesterases</i> <b>(Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. Eds) Plenum Publishing Corp.</b>: 232<br /><br />Mendelson I, Kronman C, Zeliger N, Seri T, Ordentlich A, Shafferman A, Velan B (1998) <br />Bovine Acetylcholinestrase  Cloning, Expression and Characterization of the Recombinant Enzyme<br /><i>In: Structure and Function of Cholinesterases and Related Proteins - Proceedings of Sixth International Meeting on Cholinesterases</i> <b>(Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. Eds) Plenum Publishing Corp.</b>: 148<br /><br />Mendelson I, Kronman C, Ariel N, Shafferman A, Velan B (1998) <br />Bovine acetylcholinesterase: cloning, expression and characterization<br /><i>Biochemical Journal</i> <b>334</b>: 251<br /><br />Ordentlich A, Barak D, Stein D, Berman D, Kronman C, Ariel N, Velan B, Shafferman A (1998) <br />Does Electrostatic Attraction or Steering by Charged Residues within the Gorge Contribute to the Reactivity of AChE?<br /><i>In: Structure and Function of Cholinesterases and Related Proteins - Proceedings of Sixth International Meeting on Cholinesterases</i> <b>(Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. Eds) Plenum Publishing Corp.</b>: 234<br /><br />Ordentlich A, Barak R, Barak D, Fischer M, Benschop HP, De Jong LP, Segall Y, Velan B, Shafferman A (1998) <br />ESMS as a Unique Tool for the Molecular Monitoring of Reactions between HuAChE and Various OP-Agents<br /><i>In: Structure and Function of Cholinesterases and Related Proteins - Proceedings of Sixth International Meeting on Cholinesterases</i> <b>(Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. Eds) Plenum Publishing Corp.</b>: 249<br /><br />Ordentlich A, Barak D, Kronman C, Ariel N, Segall Y, Velan B, Shafferman A (1998) <br />Functional characteristics of the oxyanion hole in human acetylcholinesterase<br /><i>Journal of Biological Chemistry</i> <b>273</b>: 19509<br /><br />Shafferman A, Ordentlich A, Barak D, Kronman C, Ariel N, Velan B (1998) <br />Contribution of the Active Center Functional Architecture to AChE Reactivity Toward Substrates and Inhibitors<br /><i>In: Structure and Function of Cholinesterases and Related Proteins - Proceedings of Sixth International Meeting on Cholinesterases</i> <b>(Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. Eds) Plenum Publishing Corp.</b>: 203<br /><br />Velan B, Kronman C, Chitlaru T, Mendelson I, Ordentlich A, Shafferman A (1998) <br />Contribution of Primary Sequence and Post-Translation Modification to the Pharmacokinetics of Human and Bovine Acetylcholinesterases<br /><i>In: Structure and Function of Cholinesterases and Related Proteins - Proceedings of Sixth International Meeting on Cholinesterases</i> <b>(Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. Eds) Plenum Publishing Corp.</b>: 291<br /><br />Barak R, Ordentlich A, Barak D, Fischer M, Benschop HP, De Jong LP, Segall Y, Velan B, Shafferman A (1997) <br />Direct determination of the chemical composition of acetylcholinesterase phosphonylation products utilizing electrospray- ionization mass spectrometry<br /><i>FEBS Letters</i> <b>407</b>: 347<br /><br />Shafferman A, Ordentlich A, Barak D, Stein D, Ariel N, Velan B (1997) <br />Aging of somanyl-acetylcholinesterase adducts: facts and models [letter]<br /><i>Biochemical Journal</i> <b>324</b>: 996<br /><br />Grosfeld H, Barak D, Ordentlich A, Velan B, Shafferman A (1996) <br />Interactions of oxime reactivators with diethylphosphoryl adducts of human acetylcholinesterase and its mutant derivatives<br /><i>Molecular Pharmacology</i> <b>50</b>: 639<br /><br />Ordentlich A, Barak D, Kronman C, Ariel N, Segall Y, Velan B, Shafferman A (1996) <br />The architecture of human acetylcholinesterase active center probed by interactions with selected organophosphate inhibitors<br /><i>Journal of Biological Chemistry</i> <b>271</b>: 11953<br /><br />Shafferman A, Ordentlich A, Barak D, Stein D, Ariel N, Velan B (1996) <br />Aging of phosphylated human acetylcholinesterase: catalytic processes mediated by aromatic and polar residues of the active centre<br /><i>Biochemical Journal</i> <b>318</b>: 833<br /><br />Velan B, Barak D, Ariel N, Leitner M, Bino T, Ordentlich A, Shafferman A (1996) <br />Structural modifications of the omega loop in human acetylcholinesterase<br /><i>FEBS Letters</i> <b>395</b>: 22<br /><br />Barak D, Ordentlich A, Kronman C, Ber R, Bino T, Ariel N, Osman R, Velan B, Shafferman A (1995) <br />Electrostatic Attraction by Surface Charge does not Contribute to the Catalytic Efficiency of Acetylcholinesterase<br /><i>In Enzyme of the Cholinesterase Family - Proceedings of Fifth International Meeting on Cholinesterases</i> <b>(Quinn, D.M., Balasubramanian, A.S., Doctor, B.P., Taylor, P., Eds) Plenum Publishing Corp.</b>: 223<br /><br />Barak D, Ordentlich A, Bromberg A, Kronman C, Marcus D, Lazar A, Ariel N, Velan B, Shafferman A (1995) <br />Allosteric modulation of acetylcholinesterase activity by peripheral ligands involves a conformational transition of the anionic subsite<br /><i>Biochemistry</i> <b>34</b>: 15444<br /><br />Kronman C, Flashner Y, Shafferman A, Velan B (1995) <br />Signal-Mediated Cellular Retention and Subunit Assembly of Human Acetylcholinesterase<br /><i>In Enzyme of the Cholinesterase Family - Proceedings of Fifth International Meeting on Cholinesterases</i> <b>(Quinn, D.M., Balasubramanian, A.S., Doctor, B.P., Taylor, P., Eds) Plenum Publishing Corp.</b>: 293<br /><br />Kronman C, Velan B, Marcus D, Ordentlich A, Reuveny S, Shafferman A (1995) <br />Involvement of oligomerization, N-glycosylation and sialylation in the clearance of cholinesterases from the circulation<br /><i>Biochemical Journal</i> <b>311</b>: 959<br /><br />Lebleu M, Clery C, Masson P, Reuveny S, Marcus D, Velan B, Shafferman A (1995) <br />Denaturation of Recombinant Human Acetylcholinesterase<br /><i>In Enzyme of the Cholinesterase Family - Proceedings of Fifth International Meeting on Cholinesterases</i> <b>(Quinn, D.M., Balasubramanian, A.S., Doctor, B.P., Taylor, P., Eds) Plenum Publishing Corp.</b>: 131<br /><br />Ordentlich A, Kronman C, Stein D, Ariel N, Reuveny S, Marcus D, Segall Y, Barak D, Velan B, Shafferman A (1995) <br />Amino Acids Determining Specificity to OP-Agents and Facilitating the Aging Process in Human Acetylcholinesterase<br /><i>In Enzyme of the Cholinesterase Family - Proceedings of Fifth International Meeting on Cholinesterases</i> <b>(Quinn, D.M., Balasubramanian, A.S., Doctor, B.P., Taylor, P., Eds) Plenum Publishing Corp.</b>: 221<br /><br />Ordentlich A, Barak D, Kronman C, Ariel N, Segall Y, Velan B, Shafferman A (1995) <br />Contribution of aromatic moieties of tyrosine 133 and of the anionic subsite tryptophan 86 to catalytic efficiency and allosteric modulation of acetylcholinesterase<br /><i>Journal of Biological Chemistry</i> <b>270</b>: 2082<br /><br />Shafferman A, Ordentlich A, Barak D, Kronman C, Ariel N, Leitner M, Segall Y, Bromberg A, Reuveny S, Marcus D, Bino T, Lazar A, Cohen S, Velan B (1995) <br />Molecular Aspects of Catalysis and of Allosteric Regulation of Aceytlcholinesterases<br /><i>In Enzyme of the Cholinesterase Family - Proceedings of Fifth International Meeting on Cholinesterases</i> <b>(Quinn, D.M., Balasubramanian, A.S., Doctor, B.P., Taylor, P., Eds) Plenum Publishing Corp.</b>: 189<br /><br />Velan B, Kronman C, Ordentlich A, Flashner Y, Ber R (1995) <br />Post-Translation Processing of Acetylcholinesterase<br /><i>In Enzyme of the Cholinesterase Family - Proceedings of Fifth International Meeting on Cholinesterases</i> <b>(Quinn, D.M., Balasubramanian, A.S., Doctor, B.P., Taylor, P., Eds) Plenum Publishing Corp.</b>: 269<br /><br />Ashani Y, Grunwald J, Kronman C, Velan B, Shafferman A (1994) <br />Role of tyrosine 337 in the binding of huperzine A to the active site of human acetylcholinesterase<br /><i>Molecular Pharmacology</i> <b>45</b>: 555<br /><br />Barak D, Kronman C, Ordentlich A, Ariel N, Bromberg A, Marcus D, Lazar A, Velan B, Shafferman A (1994) <br />Acetylcholinesterase peripheral anionic site degeneracy conferred by amino acid arrays sharing a common core<br /><i>Journal of Biological Chemistry</i> <b>269</b>: 6296<br /><br />Kronman C, Ordentlich A, Barak D, Velan B, Shafferman A (1994) <br />The back door hypothesis for product clearance in acetylcholinesterase challenged by site-directed mutagenesis<br /><i>Journal of Biological Chemistry</i> <b>269</b>: 27819<br /><br />Seidman S, Aziz-Aloya RB, Timberg R, Loewenstein Y, Velan B, Shafferman A, Liao J, Norgaard-Pedersen B, Brodbeck U, Soreq H (1994) <br />Overexpressed monomeric human acetylcholinesterase induces subtle ultrastructural modifications in developing neuromuscular junctions of Xenopus laevis embryos<br /><i>Journal of Neurochemistry</i> <b>62</b>: 1670<br /><br />Shafferman A, Ordentlich A, Barak D, Kronman C, Ber R, Bino T, Ariel N, Osman R, Velan B (1994) <br />Electrostatic attraction by surface charge does not contribute to the catalytic efficiency of acetylcholinesterase<br /><i>EMBO Journal</i> <b>13</b>: 3448<br /><br />Velan B, Kronman C, Flashner Y, Shafferman A (1994) <br />Reversal of signal-mediated cellular retention by subunit assembly of human acetylcholinesterase<br /><i>Journal of Biological Chemistry</i> <b>269</b>: 22719<br /><br />Kerem A, Kronman C, Bar-Nun S, Shafferman A, Velan B (1993) <br />Interrelations between assembly and secretion of recombinant human acetylcholinesterase<br /><i>Journal of Biological Chemistry</i> <b>268</b>: 180<br /><br />Lazar A, Reuveny S, Kronman C, Velan B, Shafferman A (1993) <br />Evaluation of anchorage-dependent cell propagation systems for production of human acetylcholinesterase by recombinant 293 cells<br /><i>Cytotechnology</i> <b>13</b>: 115<br /><br />Ordentlich A, Barak D, Kronman C, Flashner Y, Leitner M, Segall Y, Ariel N, Cohen S, Velan B, Shafferman A (1993) <br />Dissection of the human acetylcholinesterase active center determinants of substrate specificity. Identification of residues constituting the anionic site, the hydrophobic site, and the acyl pocket<br /><i>Journal of Biological Chemistry</i> <b>268</b>: 17083<br /><br />Ordentlich A, Kronman C, Barak D, Stein D, Ariel N, Marcus D, Velan B, Shafferman A (1993) <br />Engineering resistance to 'aging' of phosphylated human acetylcholinesterase. Role of hydrogen bond network in the active center<br /><i>FEBS Letters</i> <b>334</b>: 215<br /><br />Shafferman A, Velan B, Barak D, Kronman C, Ordentlich A, Flashner Y, Leitner M, Segal Y, Grosfeld H, Stein D, Ariel N (1993) <br />Recombinant human acetylcholinesterase - Enzyme engineering<br /><i>Medical Defense Bioscience Review</i> <b>3</b>: 1097<br /><br />Velan B, Kronman C, Ordentlich A, Flashner Y, Leitner M, Cohen S, Shafferman A (1993) <br />N-glycosylation of human acetylcholinesterase: effects on activity, stability and biosynthesis<br /><i>Biochemical Journal</i> <b>296</b>: 649<br /><br />Barak D, Ariel N, Velan B (1992) <br />Molecular Models for Human AChE and its Phosphonylation Products<br /><i>In Multidisciplinary approaches to cholinesterase functions - Proceedings of Fourth International Meeting on Cholinesterases</i> <b>(Shafferman, A. and Velan, B., Eds) Plenum Press, New York</b>: 195<br /><br />Kronman C, Velan B, Gozes Y, Leitner M, Flashner Y, Lazar A, Marcus D, Sery T, Papier Y, Grosfeld H, Cohen S, Shafferman A (1992) <br />Production and secretion of high levels of recombinant human acetylcholinesterase in cultured cell lines: microheterogeneity of the catalytic subunit<br /><i>Gene</i> <b>121</b>: 295<br /><br />Shafferman A, Velan B (1992) <br />Acetylcholinesterase Catalysis - Protein Engineering Studies<br /><i>In Multidisciplinary approaches to cholinesterase functions - Proceedings of Fourth International Meeting on Cholinesterases</i> <b>(Shafferman, A. and Velan, B., Eds) Plenum Press, New York</b>: 165<br /><br />Shafferman A, Kronman C, Flashner Y, Leitner M, Grosfeld H, Ordentlich A, Gozes Y, Cohen S, Ariel N, Barak D, Harel M, Silman I, Sussman JL, Velan B (1992) <br />Mutagenesis of human acetylcholinesterase. Identification of residues involved in catalytic activity and in polypeptide folding<br /><i>Journal of Biological Chemistry</i> <b>267</b>: 17640<br /><br />Shafferman A, Velan B, Ordentlich A, Kronman C, Grosfeld H, Leitner M, Flashner Y, Cohen S, Barak D, Ariel N (1992) <br />Substrate inhibition of acetylcholinesterase: residues affecting signal transduction from the surface to the catalytic center<br /><i>EMBO Journal</i> <b>11</b>: 3561<br /><br />Velan B, Kronman C, Leitner M (1992) <br />Molecular Organization of Recombinant Human Acetylcholinesterase<br /><i>In Multidisciplinary approaches to cholinesterase functions - Proceedings of Fourth International Meeting on Cholinesterases</i> <b>(Shafferman, A. and Velan, B., Eds) Plenum Press, New York</b>: 39<br /><br />Velan B, Grosfeld H, Kronman C, Leitner M, Gozes Y, Lazar A, Flashner Y, Marcus D, Cohen S, Shafferman A (1991) <br />The effect of elimination of intersubunit disulfide bonds on the activity, assembly, and secretion of recombinant human acetylcholinesterase. Expression of acetylcholinesterase Cys-580----Ala mutant<br /><i>Journal of Biological Chemistry</i> <b>266</b>: 23977<br /><br />Velan B, Kronman C, Grosfeld H, Leitner M, Gozes Y, Flashner Y, Sery T, Cohen S, Ben-Aziz R, Seidman S, Shafferman A, Soreq H (1991) <br />Recombinant human acetylcholinesterase is secreted from transiently transfected 293 cells as a soluble globular enzyme<br /><i>Cellular Molecular Neurobiology</i> <b>11</b>: 143<br /><br />Ariel N, Lehrer S, Elhanaty E, Sabo T, Brodt P, Lachmi B, Katz D, Levin R, Grosfeld H, Velan B, et al. (1990) <br />Serologically defined linear epitopes in the E2 envelope glycoprotein of Semliki Forest virus<br /><i>Arch Virol</i> <b>113</b>: 99<br /><br />Cohen S, Velan B, Grosfeld H, Shalita Z, Leitner M, Shafferman A (1985) <br />Cloning, expression and biological activity of a new variant of human interferon alpha identified in virus induced lymphoblastoid cells<br /><i>Developmental Biology Stand</i> <b>60</b>: 111<br /><br />Velan B, Cohen S, Grosfeld H, Shalita Z, Shafferman A (1985) <br />Cloning of a bovine interferon-alpha gene subfamily and comparisons between genetically engineered and leukocyte bovine interferons<br /><i>Developmental Biology Stand</i> <b>60</b>: 355<br /><br />Grosfeld H, Cohen S, Velan B, Shalita Z, Shafferman A (1984) <br />Mutations not altering the symmetrical sequences in the trp operator yield a constitutive phenotype<br /><i>Molecular & General Genetics</i> <b>195</b>: 358<br /><br />