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Family Report for: Haloalkane_dehalogenase-HLD2

Haloalkane_dehalogenase-HLD2



Relationship
Block X
Parent Family : Epoxide-hydrolase_like

Comment
Haloalkane dehalogenases (HLDs) are enzymes that catalyze the cleavage of carbon halogen bonds by a hydrolytic mechanism. The catalytic triad consists of the key nucleophile (Asp) the general base (His) and a catalytic acid (Asp or Glu). HLD where subdivided in three subfamily (Chovancova et al.) according to their substrate specificity and position of active site residues. Class one HLD1 (HLD-I) has catalytic triad Asp-His-Asp and halide-strabilizing residues Trp-Trp. Class two HLDII (HLD-II) has catalytic triad Asp-His-Glu and halide-stabilizing residues Asn-Trp. Class three HLDIII (HLD-III) has catalytic triad Asp-His-Asp and halide-strabilizing residues Asn-Trp. Although the reaction performed is different the Renilla reniformis luciferase belongs to this family. In ESTHER as in HAMAP database class I and III are merged as they are close. Luciferase from Renilla reniformis (RLuc) catalyzes the degradation of coelenterazine in the presence of molecular oxygen, resulting in the product coelenteramide, carbon dioxide, and the desired photon of light (EC 1.13.12.5). This enzyme belongs to the Haloalkane dehalogenase family II with a different catalytic function (EC 3.8.1.5) (this luciferase is different from fireflies luciferases classified as Luciferin 4-monooxygenase; ATP-dependent AMP-binding enzyme family). Reconstruction of an ancestral enzyme shows it has both hydrolase and monooxygenase activities ( Chaloupkova et al.)

Database
Sequences
Interpro
|
IPR023594 (Haloalkane dehalogenase, subfamily 2)
PIRSF
|
Pdoc
|
PFam
|
PF00561 (Abhydrolase_1)
Prints
|
Prosite
|
EC at KEGG
|
EC at EXPASY
|


Peptide in
|Fasta
Nucleotide in
|Fasta
Alignment with Multalin
|Text only/graphic display
Seed alignment with MAFFT
|No colour/coloured with Mview
Alignment with MAFFT
|No colour/coloured with Mview
Dendrogram
|Graphical display, obtained with the dnd file produced by Clustalw

References
23 more
    Title: Phylogenetic analysis of haloalkane dehalogenases
    Chovancova E, Kosinski J, Bujnicki JM, Damborsky J
    Ref: Proteins, 67:305, 2007 : PubMed

            

    Title: Evolving haloalkane dehalogenases
    Janssen DB
    Ref: Curr Opin Chemical Biology, 8:150, 2004 : PubMed

            

    Title: Repositioning the catalytic triad aspartic acid of haloalkane dehalogenase: effects on stability, kinetics, and structure
    Krooshof GH, Kwant EM, Damborsky J, Koca J, Janssen DB
    Ref: Biochemistry, 36:9571, 1997 : PubMed

            

Other Papers


Structure scheme for Haloalkane_dehalogenase-HLD2

Structures in Haloalkane_dehalogenase-HLD2 family (110)

Genes Proteins in Haloalkane_dehalogenase-HLD2 family (72)

No fragments
Substrates of some enzymes in the Haloalkane_dehalogenase-HLD2 family (34)

Inhibitors of some enzymes in the Haloalkane_dehalogenase-HLD2 family (17)



Send your questions or comments to :
Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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