Gemella sanguinis A0A2N6SEV5 protein identified as Mbeg1 in a functional multigenomic screening of human-associated bacteria for NF-kappaB-inducing bioactive effectors (Estrela et al.) since then family called Mbeg1-like Only 400 n-term are homologous to Mbeg1-like
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Bacteria: NE > Terrabacteria group: NE > Firmicutes: NE > Bacilli: NE > Bacillales: NE > Bacillales incertae sedis: NE > Bacillales Family XI. Incertae Sedis: NE > Gemella: NE > Gemella sanguinis: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MAYSDETNMNIAKEEYKNREPGYILNTEDNEYLGTLSDVNDDRTNNGEQI YTYTRTEGSDEIVSPDAPLSEREKVEEITILYRGSTAPGIGADNEDARKD WLNNDVPMAEKIMLGEKGATGQLEASSDYLKEMMEKYPNAKINIYGHSLG SMDAQYALANVTDYSRIKSANIYNGPNIYSTLTEEQKINVSALYDKINNY VDSRDLVGLGYKKGEGTVGKTYNFSGESNGINKIDQHMWGGYRFDSDGNL IDEDGKRVKAWNKPDEIKKLQAEAKNKVRDAAINKVQGLSVDVDHDGKLD AQFGRDKLLTTELMPGSGAGKDIVINFSSLQGLSKNLQGLLEDIKQIREL LTKSTTTNSTVESRKANRTETLEQSIVSYLEQINLIKSIKNLDDFYTKLE GKEKLF
The effect of the microbiota on its human host is driven, at least in part, by small-molecule and protein effectors it produces. Here, we report on the use of functional multigenomic screening to identify microbiota-encoded effectors. In this study, genomic DNA from 116 human-associated bacteria was cloned en masse, and the resulting multigenomic library was screened using a nuclear factor-kappaB reporter (NF-kappaB) assay. Functional multigenomics builds on the concept of functional metagenomics but takes advantage of increasing advances in cultivating and sequencing human-associated bacteria. Effector genes found to confer NF-kappaB-inducing activity to Escherichia coli encode proteins in four general categories: cell wall hydrolases, membrane transporters, lipopolysaccharide biosynthetic enzymes, and proteins of unknown function. The compact nature of multigenomic libraries, which results from the ability to normalize input DNA ratios, should simplify screening of libraries using diverse heterologous hosts and reporter assays, increasing the rate of discovery of novel effector genes.IMPORTANCE Human-associated bacteria are thought to encode bioactive small molecules and proteins that play an intimate role in human health and disease. Here, we report on the creation and functional screening of a multigenomic library constructed using genomic DNA from 116 bacteria found at diverse sites across the human body. Individual clones were screened for genes capable of conferring NF-kappaB-inducing activity to Escherichia coli NF-kappaB is a useful reporter for a range of cellular processes related to immunity, pathogenesis, and inflammation. Compared to the screening of metagenomic libraries, the ability to normalize input DNA ratios when constructing a multigenomic library should facilitate the more efficient examination of commensal bacteria for diverse bioactivities. Multigenomic screening takes advantage of the growing available resources in culturing and sequencing the human microbiota and generates starting points for more in-depth studies on the mechanisms by which commensal bacteria interact with their human host.
