Gene_locus Report for: 9bact-a0a1u9wz58

uncultured bacterium Sibe-eh Alpha/beta epoxide hydrolase

Sibe-eh Novel epoxide hydrolase belonging to the alpha/beta hydrolases superfamily in metagenomes from ORF found in the Russian metagenome hot environments

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms: N E > Bacteria: N E > environmental samples: N E > uncultured bacterium: N E

5_AlphaBeta_hydrolase : 9bact-estC55.165uncultured bacterium. Lipolytic protein estC55-165, 9bact-estC55.241uncultured bacterium. Lipolytic protein estC55-241, 9bact-estC76.137uncultured bacterium. Lipolytic protein estC76-137.
6_AlphaBeta_hydrolase : 9bact-d8v1i9uncultured bacterium Esterase/lipase clone EM3L6, 9bact-d8v1i5uncultured bacterium Esterase/lipase clone EM3L1, 9bact-i3rtt4uncultured bacterium EstL28 cold-active Esterase, 9bact-a0a0n9qma1uncultured bacterium esterase est1, 9bact-a0a1s5qki8uncultured bacterium EH127 Alpha/beta hydrolase fold carboxyl esterase, 9bact-a0a1s5qm34uncultured bacterium EH113 Alpha/beta hydrolase fold carboxyl esterase, 9bact-r9qzg0uncultured bacterium EH109 Hybrid C-C meta-cleavage hydrolase-carboxylesterase, 9gamm-KY483646Uncultured bacterium EH57 Alpha/beta hydrolase fold, 9bact-KY458160Uncultured bacterium EH53 clone 2085D pCC1FOS Lip088, 9bact-a0a1s5qm38uncultured bacterium, EH26,clone MGS-ElMaxE1 Alpha/beta hydrolase fold carboxyl esterase, 9bact-a0a0h4tgu6uncultured bacterium EH58 Alpha/beta hydrolase fold protein LIPESV12_9 LIPESV12_24, 9zzzz-g3crf3uncultured bacteria Metagenomic AB hydrolase-1 domain-containing protein, 9bact-i6yrg4 uncultured bacterium Est816 N-Acyl homoserine lactone degrading enzyme, 9zzzz-A0A0G3FEJ8 uncultured bacteria Metagenomic carboxyl esterase MGS0156, 9bact-a2a1y4uncultured bacterium putative prolyl aminopeptidase, 9bact-d3ykv3uncultured bacterium Lip, 9bact-q3ymm0uncultured bacterium lipase/esterase, 9bact-q3ymm6uncultured bacterium lipase/esterase, 9bact-q3ymm8uncultured bacterium lipase/esterase, 9bact-q3ymm9uncultured bacterium lipase/esterase, 9bact-MGS-M2 uncultured bacterium EH107 alpha/beta hydrolase enzyme MGS-M2, 9bact-estC55.25uncultured bacterium. Lipolytic protein estC55-25, 9bact-estC55.76uncultured bacterium. Lipolytic protein estC55-76, 9bact-estC55.20uncultured bacterium. Lipolytic protein, 9bact-estC55.4n1uncultured bacterium. Lipolytic protein, 9bact-estC76.263uncultured bacterium. Lipolytic protein estC55-100 estC76-263, 9bact-estC55.227uncultured bacterium. Lipolytic protein estC55-227, 9bact-estC55.159uncultured bacterium. Lipolytic protein estC55-159, 9bact-estC55.51uncultured bacterium. Lipolytic protein estC55-51, 9bact-estC55.31uncultured bacterium. Lipolytic protein estC55-31, 9bact-estC55.215uncultured bacterium. Lipolytic protein estC55-215, 9bact-estC55.244uncultured bacterium. Lipolytic protein estC55-244, 9bact-estC55.81uncultured bacterium. Lipolytic protein estC55-81, 9bact-estC55.15uncultured bacterium. Lipolytic protein estC55-15, 9bact-estC55.231uncultured bacterium. Lipolytic protein estC55-231, 9bact-estC76.266n2uncultured bacterium. Lipolytic protein estC76-28_2 estC76-266_2, 9bact-estC76.28n1uncultured bacterium. Lipolytic protein estC76.28_1, 9bact-estC76.177uncultured bacterium. Lipolytic protein, 9bact-estC76.266n1uncultured bacterium. Lipolytic protein estC76-266_1, 9bact-estC76.248uncultured bacterium. Lipolytic protein estC76-248.
A85-EsteraseD-FGH : 9bact-KY458159Uncultured bacterium EH44 clone 826H pCC1FOS Lip061, 9bact-b0fwn3uncultured bacterium Esterase A, 9bact-b5au41uncultured bacterium Esterase.
A85-Feruloyl-Esterase : 9bact-MW804671uncultured bacterium Esterase EstQ7 MW804671, 9bact-a0a0k0l6g3uncultured bacterium Esterase AS-Trib27, 9bact-a0a1y0bdl3uncultured bacterium Esterase D591, 9bact-a0a140fva9uncultured bacterium ESTGX3.
A85-IroE-IroD-Fes-Yiel : 9bact-g3c5s8uncultured bacterium. Ferric enterobactin esterase-related protein.
ABHD6-Lip : 9bact-a0a1s5ql22uncultured bacterium EH114 Alpha/beta hydrolase fold carboxyl esterase, 9bact-a0a1s5qly6uncultured bacterium EH65 Alpha/beta hydrolase fold carboxyl esterase, 9bact-d8v1i7uncultured bacterium Esterase/lipase MhpC clone EM3L3, 9bact-r9qzf7uncultured bacterium EH55 Hybrid C-C meta-cleavage hydrolase-carboxylesterase.
ABHD11-Acetyl_transferase : 9bact-a0a2s1gux0 esterase fromdeep-sea hydrothermal vent of the East Pacific Rise (EprEst) uncultured bacterium pet-28a est-OKK esterase, 9bact-a0a0k1z4z5uncultured bacterium Alpha/beta hydrolase fold protein LIPESV12_26, 9bact-g3c5s4uncultured bacterium. Alpha/beta hydrolase fold protein, 9bact-estC55.96uncultured bacterium. Lipolytic protein.
abh_upf0017 : 9bact-q3ymm2uncultured bacterium Lipase/esterase.
abh_upf00227 : 9bact-h8xw46uncultured bacterium. Carboxylesterase.
Acetyl-esterase_deacetylase : 9bact-KY458164Uncultured bacterium EH70 clone 27C11 pCC1FOS Lip113.
Aclacinomycin-methylesterase_RdmC : 9bact-a9ukk2uncultured bacterium Lipase/esterase, 9bact-b0l3i3uncultured bacterium Lipase/esterase, 9bact-b8y566uncultured bacterium Lipolytic enzyme, 9bact-c3rww3uncultured bacterium Putative lipase/esterase, uncba-Q9KIU0uncultured bacterium esterase, 9bact-g3c5t0uncultured bacterium. Lipase/esterase, 9bact-g3c5s9uncultured bacterium. Lipase/esterase, 9bact-estC55.197uncultured bacterium. Lipolytic protein.
AlphaBeta_hydrolase : 9bact-Aii810uncultured bacterium Cold-adapted N-acylhomoserine lactonase Aii810, 9bact-KY458167uncultured bacterium EH130 clone 83E4 pCC1FOS Lip139, 9bact-KY203031uncultured bacterium EH74 clone 00041_62_12ROV11 esterase/lipase, 9bact-h8xw45uncultured bacterium. Carboxylesterase, 9bact-estC55.12uncultured bacterium. Lipolytic protein estC55-12, 9bact-estC76.221uncultured bacterium. Lipolytic protein.
Bacterial_Est97 : 9bact-Est97 uncultured bacterium cold-adapted esterase from an Arctic intertidal metagenomic library.
Bacterial_esterase : uncba-Q848M3uncultured bacterium hypothetical protein.
Bacterial_lip_FamI.1 : 9bact-g1apt8 uncultured bacterium LipC12, a true lipase isolated through a metagenomics approach.
Bacterial_lip_FamI.3 : 9bact-q6xci1uncultured bacterium lipb, 9bact-q6xci2uncultured bacterium lipa.
Bacterial_lip_FamI.5 : 9bact-a4uz10uncultured bacterium PLA depolymerase pLA-M4 pLAM4.
Bact_LipEH166_FamXII : 9bact-b1pz93uncultured bacterium Lipase.
BD-FAE : 9prot-EH41Uncultured bacterium EH41 SRA059294 esterase/lipase, 9bact-CEUbrbuncultured bacterium carbamate degrading enzyme active on Impranil poly-urethane polyester polyurethane PUR, 9zzzz-g3crg0uncultured bacteria Metagenomic Abhydrolase_3 domain-containing protein, 9bact-q6rjl2uncultured bacterium lipase/esterase, 9bact-MGS-M1 uncultured bacterium EH115 alpha/beta hydrolase enzyme MGS-M1, 9bact-a0a345g0q2uncultured bacterium. Est903 Putative esterase, 9bact-estC55.253uncultured bacterium. Lipolytic protein estC55-253, 9bact-estC55.57uncultured bacterium. Lipolytic protein.
BioH : 9bact-q3ymm4uncultured bacterium lipase/esterase, 9bact-q6a3q9uncultured bacterium lip1 protein, 9bact-estC76.202uncultured bacterium. Lipolytic protein estC76-202.
CarbLipBact_2 : 9bact-KY203034Uncultured bacterium EH111 clone 00002_36_12ROV11 esterase/lipase, 9bact-k2dnl8uncultured bacterium. Uncharacterized protein, 9bact-k2f369uncultured bacterium. Uncharacterized protein, 9bact-k2f264uncultured bacterium. Uncharacterized protein, 9bact-k2gak8uncultured bacterium. Uncharacterized protein, 9bact-k2age0uncultured bacterium. Uncharacterized protein, 9bact-k2fcq4uncultured bacterium. Uncharacterized protein, 9bact-k2fvb3uncultured bacterium. Uncharacterized protein, 9bact-r9rev7uncultured bacterium. Esterase.
Carbon-carbon_bond_hydrolase : 9bact-a0a1s5qkm4uncultured bacterium EH63 Alpha/beta hydrolase fold carboxyl esterase, 9bact-a0a1s5qke6uncultured bacterium EH38 Alpha/beta hydrolase fold carboxyl esterase, 9bact-a0a1s5qkf3uncultured bacterium EH36 Alpha/beta hydrolase fold carboxyl esterase, 9bact-a0a1s5qkl2Uncultured bacterium, EH33, clone MGS-MesE6 alpha/beta hydrolase fold carboxyl esterase, 9bact-a0a1s5qmi7Uncultured bacterium, EH17, clone MGS-AqE1 alpha/beta hydrolase fold carboxyl esterase, 9bact-MChE2 Uncultured bacterium MGS-MChE2,alpha/beta hydrolase enzyme from sediment sample of lagoon of Mar Chica, Morocco, 9bact-c6kti0uncultured bacterium 2-hydroxymuconic semialdehyde hydrolase, 9bact-c6ktz1uncultured bacterium 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase, 9bact-c6ku85uncultured bacterium 2-hydroxymuconic semialdehyde hydrolase, 9bact-c6kub4uncultured bacterium 2-hydroxymuconic semialdehyde hydrolase, 9bact-c6kud7uncultured bacterium 2-hydroxymuconic semialdehyde hydrolase, 9bact-c6kwi7uncultured bacterium 2-hydroxymuconic semialdehyde hydrolase, 9bact-c6l0y5uncultured bacterium 2-hydroxymuconic semialdehyde hydrolase, 9bact-estC55.2uncultured bacterium. Lipolytic protein, 9bact-estC55.97uncultured bacterium. Lipolytic protein estC55-97.
Carboxymethylbutenolide_lactonase : 9bact-estC55.34uncultured bacterium. Lipolytic protein estC55-34.
Carb_B_Bacteria : 9bact-102Pee006uncultured bacterium 176763, pEE006 102 YNP6_02150 Environmental sample, 9bact-a0a1s5y1h9uncultured bacterium. Carboxylic ester hydrolase, 9bact-a0a1s5qkj8uncultured bacterium EH139 Carboxylic ester hydrolase, 9bact-a0a1s5qkg4uncultured bacterium EH64 Carboxylic ester hydrolase, 9actn-KY010298Uncultured bacterium, EH20 carboxyl esterase, 9bact-b8y554uncultured bacterium Lipolytic enzyme, 9bact-c6ku06uncultured bacterium Carboxylesterase, 9bact-c7f9k0uncultured bacterium Lipase/esterase, 9bact-d2xv58uncultured bacterium Carboxylesterase, 9bact-g1ard1uncultured bacterium EstDL30, 9bact-h9lcg9uncultured bacterium Est2, 9bact-i2b2s6uncultured bacterium Carboxylesterase, 9bact-q3ymm5uncultured bacterium lipase/esterase, 9bact-j7i2s6uncultured bacterium Carboxylesterase type B, 9bact-j7hzn3uncultured bacterium Thermostable carboxylesterase, 9bact-j7hzn6uncultured bacterium Thermostable carboxylesterase, 9bact-j7i2s2uncultured bacterium Thermostable carboxylesterase, 9bact-j7i5y7uncultured bacterium Carboxylesterase type B, 9bact-j7i6j5uncultured bacterium Carboxylesterase type B, 9bact-k2eq93uncultured bacterium Uncharacterized protein, 9bact-s5tby8uncultured bacterium Esterase, 9bact-a0a097i5b4uncultured bacterium. Carboxylic ester hydrolase, 9bact-a0a0a7eq69uncultured bacterium. Carboxylic ester hydrolase, 9bact-estC76.136uncultured bacterium. Carboxylic ester hydrolase estC76-136, 9bact-estC55.118uncultured bacterium. Carboxylic ester hydrolase estC55-118, 9bact-estC55.62uncultured bacterium. Carboxylic ester hydrolase estC55-62, 9bact-estC55.52uncultured bacterium. Carboxylic ester hydrolase estC55-52, 9bact-estC55.3uncultured bacterium. Carboxylic ester hydrolase estC55-3.
Chlorophyllase : 9bact-a0a1b2ypb1uncultured bacterium. Uncharacterized protein, 9bact-b5au40uncultured bacterium. Lipase, 9bact-estC55.24uncultured bacterium. Lipolytic protein estC55-24.
Dienelactone_hydrolase : 9bact-KP212148 Uncultured bacterium Dienelactone Hydrolase-like Promiscuous Phosphotriesterase P91 from Metagenomic Libraries.
Duf_452 : 9bact-w1i648environmental samples uncultured bacterium Uncultured bacterium extrachromosomal DNA RGI01476.
Duf_3089 : 9bact-MT859404Uncultured bacterium Est3S from cow rumen metagenomic library with organosphosphorus insecticides degrading capability, 9bact-d5l5q1uncultured bacterium EstGK1, 9bact-d5l5q2uncultured bacterium EstZ3 EstGK1, 9bact-d8v1i6uncultured bacterium Esterase/lipase clone EM3L2, 9bact-e2d695uncultured bacterium EstD2, 9bact-q0gpb3uncultured bacterium Est5S, 9bact-a0a077l869uncultured bacterium. Uncharacterized protein, 9bact-j7hz76uncultured bacterium. Esterase, 9bact-j7i5z1uncultured bacterium. Esterase, 9bact-j7i6j9uncultured bacterium. Esterase, 9bact-j7i6k3uncultured bacterium. Esterase, 9bact-k2cj78uncultured bacterium. Uncharacterized protein, 9bact-k9ryb0uncultured bacterium. EstWSD, 9bact-a0a088s6n7uncultured bacterium. Esterase Est10.
Epoxide_hydrolase : 9bact-a0a1u9wz52 uncultured bacterium CH65-eh Alpha/beta epoxide hydrolase, 9bact-a0a1s5qkk8Uncultured bacterium, EH32, clone MGS-MilE2 alpha/beta hydrolase fold carboxyl esterase, 9bact-a8wbx9uncultured bacterium Epoxide hydrolase, 9bact-estC55.19n2uncultured bacterium. Lipolytic protein, 9bact-estC55.8n2uncultured bacterium. Lipolytic protein.
Est-OsmC : 9bact-g3c5t8uncultured bacterium. esterase is found in n-term of an OsmC/Ohr family domain.
Est9X : 9bact-j9vdv8uncultured bacterium Esterase.
Esterase_phb : 9bact-d8v1i8uncultured bacterium Esterase/lipase EM3L4, 9bact-estC55.77uncultured bacterium. Lipolytic protein estC55-77.
FaeC : 9bact-a0a0u2zp04uncultured bacterium. Ricin-type beta-trefoil lectin domain protein, 9bact-estC55.42uncultured bacterium. Lipolytic protein estC55-42.
Fungal-Bact_LIP : 9bact-estC55.154uncultured bacterium. Lipolytic protein estC55-154, 9bact-b0ln78uncultured bacterium Esterase, uncba-Q9KIU2uncultured bacterium esterase.
GTSAGmotif : 9bact-a0a142j6i6 uncultured bacterium esterase PMGL2 with GCSAG motif from a permafrost metagenomic library., 9bact-b8y553uncultured bacterium Lipolytic enzyme, 9bact-b1pz92uncultured bacterium Esterase, 9bact-d8v1j5uncultured bacterium Esterase, 9bact-d8v1j4uncultured bacterium Esterase, 9bact-d8v1j3uncultured bacterium Esterase, 9bact-d7nj22uncultured bacterium alkaline-stable lipase, 9bact-d5mt93uncultured bacterium Esterase, 9bact-a3rgw9uncultured bacterium Putative esterase, 9bact-h6bdx2uncultured bacterium Lipolytic enzyme, 9bact-b8y558uncultured bacterium Lipolytic enzyme, 9bact-b8y563uncultured bacterium Lipolytic enzyme.
Haloalkane_dehalogenase-HLD1 : 9bact-v5ln96uncultured bacterium Haloalkane dehalogenase Cluster B eHLD-B, 9bact-v5llk8uncultured bacterium. Haloalkane dehalogenase dhmA, 9bact-v5llz0uncultured bacterium. Haloalkane dehalogenase.
Haloalkane_dehalogenase-HLD2 : 9bact-AncHLD3 Putative ancestral haloalkane dehalogenase AncHLD3 (node 3), 9bact-AncHLD2 Putative ancestral haloalkane dehalogenase AncHLD2 (node 2), 9bact-AncHLD5 Putative ancestral haloalkane dehalogenase AncHLD5 (node 5), 9bact-v5llz5uncultured bacterium Haloalkane dehalogenase Cluster C eHLD-C.
HNLyase_Bact : 9bact-c7f9k4uncultured bacterium Lipase/esterase, 9bact-estC55.167uncultured bacterium. Lipolytic protein estC55-167.
Homoserine_transacetylase : 9bact-a0a1b1h1k0 uncultured bacterium; Alteromonas macleodii; Alteromonas marina, Homoserine O-acetyltransferase Est22 esterase not transferase, 9bact-g3c5t3uncultured bacterium. Lipase/esterase.
Hormone-sensitive_lipase_like : 9bact-PersiLipase1uncultured bacterium alkaline, thermostable, detergent compatible and organic solvent tolerant lipase PersiLipase1, 9bact-EH0 uncultured bacterium ester-hydrolase EH0 from the metagenome of Sorghum bicolor rhizosphere from the Henfaes research centre (Gwynedd Wales), 9bact-b8y561uncultured bacterium Lipolytic enzyme lip10, 9bact-estGX1uncultured bacterium Esterase GX1 estGX1, 9bact-EstATIIuncultured bacterium; Pseudomonas sp. Thermophilic, psychrostable heavy metal-resistant Red Sea Brine Pool Esterase EstATII, 9bact-MZ484407Uncultured bacterium clone YH-E8 family IV esterase (est8L), 9bact-a0a0n9qch2uncultured bacterium Halotolerant esterase, 9bact-Est8.6Y9K Esterase EST8 with transacylase activity (different from Est8 4YPV), 9bact-a9ukj9uncultured bacterium Lipase/esterase, 9bact-g8enl6uncultured bacterium moderately thermophilic solvent-tolerant esterase, 9bact-a0a1x9zm83uncultured bacterium RPSP24 esterase, 9bact-bol3I1uncultured bacterium. Lipase/esterase, 9zzzz-a0a2k8jn75 uncultured bacterium ester-hydrolase EH3 from the metagenome of marine sediments at Milazzo harbor (Sicily, Italy), 9zzzz-a0a2k8jt94uncultured bacterium EH2 Uncharacterized protein, 9bact-a0a1s5ql04EH67 uncultured bacterium Alpha/beta hydrolase fold carboxyl esterase, 9bact-KY458168Uncultured bacterium EH48 clone 38H9 pCC1FOS Lip142, 9bact-t1vzd5uncultured bacterium EH42 clone L-Tri-RimGill-1 Esterase, 9prot-SRP030024Uncultured bacterium EH35 clone Alpha/beta hydrolase fold carboxyl esterase, 9prot-KY019260Uncultured bacterium, EH29 clone Alpha/beta hydrolase fold carboxyl esterase, 9prot-KY010302Uncultured bacterium, EH22, clone Alpha/beta hydrolase fold carboxyl esterase, 9bact-KY010297Uncultured bacterium, EH15, clone Alpha/beta hydrolase fold carboxyl esterase EstA1, 9bact-KY483642Uncultured bacterium, EH14, clone Alpha/beta hydrolase fold carboxyl esterase, 9bact-a0a1s5qkj1Uncultured bacterium, EH12, clone MGS-BizE3 Alpha/beta hydrolase fold carboxyl esterase, 9bact-KY010299Uncultured bacterium, EH11, KY010299 alpha/beta hydrolase fold, 9bact-KY483648Uncultured bacterium, EH9, KY483648 alpha/beta hydrolase fold, 9bact-a0a1s5qf1Uncultured bacterium, EH4, clone MGS-MilE5 alpha/beta hydrolase fold, 9bact-a0a1s5qkg0uncultured bacterium clone MGS-ElMaxE6 EH5 Alpha/beta hydrolase fold carboxyl esterase, 9zzzz-g3crd1uncultured bacteria Metagenomic carboxyl esterase Est06, 9bact-H6BDX1 uncultured bacterium esterase Est22, 9bact-MilE3 Uncultured bacterium MGS-MilE3,EH37 alpha/beta hydrolase enzyme from sediment sample of Milazzo Harbor, Italy, 9bact-LAE6 Uncultured bacterium esterase LAE6 EH1 EH1AB1 alpha/beta hydrolase enzyme from the metagenome of Lake Arreo, Spain, 9bact-e7djy5 Uncultured bacterium feruloyl esterase haloalkalitolerant esterase from a soil metagenomic library Feruloyl Esterase EstF27, 9bact-Q0GMU1 Uncultured bacterium esterase/lipase (estE7) gene, complete cds, 9bact-Q0GMU2 Uncultured bacterium clone pUE5 esterase/lipase (estE5), 9bact-q3yjm9uncultured bacterium lipase, 9bact-q3ymm7uncultured bacterium lipase/esterase, 9bact-q3ymn0uncultured bacterium lipase/esterase, 9bact-q4tzq3 uncultured bacterium esterase Est25, 9bact-q6rjl0uncultured bacterium lipase/esterase, 9bact-q6rjm3uncultured bacterium lipase/esterase, bacte-esterUncultured bacterium plasmid pAH116 esterase gene, complete cds, 9bact-d7nj24uncultured bacterium Lipase/esterase, 9bact-m1plb8uncultured bacterium Esterase/lipase protein, 9bact-g3cr00uncultured bacterium. Esterase estDL26, 9bact-a0a0k0l6c0uncultured bacterium. Lipase/esterase AS-Trib6, 9bact-b8y559environmental samples uncultured bacterium Lipolytic enzyme, 9bact-b0l3i6environmental samples uncultured bacterium Lipase/esterase, 9bact-b3vp83environmental samples uncultured bacterium; Bacillus firmus Lipase/esterase, 9bact-a0a0k0l6e1environmental samples uncultured bacterium Lipase/esterase AS-Trib20-ORF2, 9bact-b0l3i4environmental samples uncultured bacterium Lipase/esterase, 9bact-b8y564environmental samples uncultured bacterium Lipolytic enzyme, 9bact-h6bdx3environmental samples uncultured bacterium Lipolytic enzyme, 9bact-k7pez9environmental samples uncultured bacterium Lipolytic enzyme SBLip2, 9bact-b8y560environmental samples uncultured bacterium Lipolytic enzyme, 9bact-a0a0k0l6e0environmental samples uncultured bacterium Lipase/esterase AS-Trib20-ORF1, 9bact-a0a0d5w6z2environmental samples uncultured bacterium Esterase EstGX2, 9bact-k7pfj8environmental samples uncultured bacterium Lipolytic enzyme SBLip5, 9bact-a0a0k0l6j0environmental samples uncultured bacterium Alpha/beta hydrolase fold-3 domain-containing protein AS-Trib23, 9bact-a0a0k0l6d5environmental samples uncultured bacterium Alpha/beta hydrolase AS-Trib12, 9bact-g3cr02 environmental samples uncultured bacterium Esterase estDL136, 9bact-estC55.268uncultured bacterium. Lipolytic protein estC55-268, 9bact-estC55.145uncultured bacterium. Lipolytic protein estC55-145, 9bact-estC55.78uncultured bacterium. Lipolytic protein estC55-78, 9bact-estC55.8n1uncultured bacterium. Lipolytic protein estC55-8_1, 9bact-estC55.56uncultured bacterium. Lipolytic protein estC55-56, 9bact-estC55.60uncultured bacterium. Lipolytic protein estC55-60, 9bact-estC55.5uncultured bacterium. Lipolytic protein estC55-5, 9bact-estC55.19n1uncultured bacterium. Lipolytic protein estC55-19_1, 9bact-estC55.229uncultured bacterium. Lipolytic protein estC55-229, 9bact-estC55.23uncultured bacterium. Lipolytic protein, 9bact-estC76.135uncultured bacterium. Lipolytic protein estC76-135.
Hydrolase-2_PEP : 9bact-k2ehy0uncultured bacterium. Esterase/lipase/thioesterase family protein.
Hydrolase_RBBP9_YdeN : 9bact-q6jwt1uncultured bacterium hypothetical protein cxs042.
Lipase_2 : 9bact-estC55.235uncultured bacterium. Lipolytic protein estC55-235, 9bact-estC55.90uncultured bacterium. Lipolytic protein estC55-90, 9bact-estC55.105uncultured bacterium. Lipolytic protein estC55-105, 9bact-estC55.151uncultured bacterium. Lipolytic protein estC55-151, 9bact-estC55.71uncultured bacterium. Lipolytic protein estC55-71, 9bact-estC55.88uncultured bacterium. Lipolytic protein estC55-88.
Lipase_bact_N_lipase : 9bact-estC55.102uncultured bacterium. Lipolytic protein estC55-102.
Monoglyceridelipase_lysophospholip : 9bact-a0a1s5qlx2uncultured bacterium EH94 Alpha/beta hydrolase fold carboxyl esterase, 9bact-a4uz14uncultured bacterium PLA depolymerase pLA-M9 pLAM9.
NLS3-Tex30 : 9bact-k1ymn0uncultured bacterium Uncharacterized protein.
PAF-Acetylhydrolase : 9bact-Lip114Uncultured bacterium clone EH40 53D4 pCC1FOS Lip114.
Pectinacetylesterase-Notum : 9bact-h9ldb8uncultured bacterium. Est5, 9bact-g3c5s0uncultured bacterium. Pectinacetylesterase/lipase/esterase, 9bact-k7qe48uncultured bacterium. EH80 LipT.
Peptidase_S37 : 9bact-k1yi15uncultured bacterium; Rikenellaceae bacterium. Uncharacterized protein.
PGAP1 : 9bact-d8v1j0uncultured bacterium Esterase/lipase clone EM3L7, 9bact-c7f9k2uncultured bacterium Lipase/esterase.
Polyesterase-lipase-cutinase : 9bact-c3ryl0 uncultured bacterium Polyethylene terephthalate hydrolyzing lipase PET2 LipIAF5-2, 9bact-estC55.95uncultured bacterium. Lipolytic protein estC55-95.
Prolyl_oligopeptidase_S9 : 9bact-KY203037uncultured bacterium EH120 clone 00152_2_12ROV11 esterase/lipase, 9bact-q6wlc7uncultured bacterium csw020.
Tannase : 9bact-e7d7j5uncultured bacterium Multi-fuction esterase tannase tan410, 9bact-estC55.156uncultured bacterium. Lipolytic protein estC55-156, 9bact-estC55.169uncultured bacterium. Lipolytic protein estC55-169, 9bact-estC55.234uncultured bacterium. Lipolytic protein estC55-234.
UCP031982 : 9bact-d8v1j6uncultured bacterium Esterase/lipase, 9bact-estC55.13uncultured bacterium. Lipolytic protein estC55-13

Title: New Thermophilic alpha/beta Class Epoxide Hydrolases Found in Metagenomes From Hot Environments
Ferrandi EE, Sayer C, De Rose SA, Guazzelli E, Marchesi C, Saneei V, Isupov MN, Littlechild JA, Monti D
Ref: Front Bioeng Biotechnol, 6:144, 2018 : PubMed
Abstract


ESTHER: Ferrandi_2018_Front.Bioeng.Biotechnol_6_144
PubMedSearch: Ferrandi 2018 Front.Bioeng.Biotechnol 6 144
PubMedID: 30386778
Gene_locus related to this paper: 9bact-a0a1u9wz52, 9bact-a0a1u9wz58

Abstract

Two novel epoxide hydrolases (EHs), Sibe-EH and CH65-EH, were identified in the metagenomes of samples collected in hot springs in Russia and China, respectively. The two alpha/beta hydrolase superfamily fold enzymes were cloned, over-expressed in Escherichia coli, purified and characterized. The new EHs were active toward a broad range of substrates, and in particular, Sibe-EH was excellent in the desymmetrization of cis-2,3-epoxybutane producing the (2R,3R)-diol product with ee exceeding 99%. Interestingly these enzymes also hydrolyse (4R)-limonene-1,2-epoxide with Sibe-EH being specific for the trans isomer. The Sibe-EH is a monomer in solution whereas the CH65-EH is a dimer. Both enzymes showed high melting temperatures with the CH65-EH being the highest at 85 degrees C retaining 80% of its initial activity after 3 h thermal treatment at 70 degrees C making it the most thermal tolerant wild type epoxide hydrolase described. The Sibe-EH and CH65-EH have been crystallized and their structures determined to high resolution, 1.6 and 1.4 A, respectively. The CH65-EH enzyme forms a dimer via its cap domains with different relative orientation of the monomers compared to previously described EHs. The entrance to the active site cavity is located in a different position in CH65-EH and Sibe-EH in relation to other known bacterial and mammalian EHs.