(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Opisthokonta: NE > Metazoa: NE > Eumetazoa: NE > Bilateria: NE > Protostomia: NE > Ecdysozoa: NE > Panarthropoda: NE > Arthropoda: NE > Mandibulata: NE > Pancrustacea: NE > Hexapoda: NE > Insecta: NE > Dicondylia: NE > Pterygota: NE > Neoptera: NE > Holometabola: NE > Diptera: NE > Nematocera: NE > Bibionomorpha: NE > Sciaroidea: NE > Sciaridae: NE > Bradysia: NE > Bradysia odoriphaga: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MEKHSGHYRFGLMPRPIRLRQLLLCTIGMLGVFDHILVECRHRELENAQI QLSQKHNSQSSSVPNDDDAFFTPYLGHGEAVRVLDAELGTLEMEQFRDTK ALTRRRGVSRRESSSHDPDDDDPMVITTDKGKIRGTVLTSGSGRKVDAWL GIPYAQPPIGALRYRLPRPIERWTGVRNATTPSNSCVQIVDVVFGDFPGA TMWNPNTPLSEDCLYINVVVPRPRPKNAPVMLWIFGGGFYSGTATLDVYD HKTLAAEENVIVVSMQYRVASLGFLYLGTPDAPGNAGLFDQNLALRWVRD NIHKFGGDPNRVTLFGESAGAVSVSMHLLSSLSRDLFQRAILESGSPTAP WALISREEAILRALRLAEAVDCPHDRHKLTDTVECLRTKDAKELVDNEWG TLGICEFPFVPVVDGAFLDETPQKSLAHGRFKKTEILTGSNTEEGYYFII YYLTELLRKEEGVTVSREEFYQAVKELNPYVNNPARQAIIFEYTDWIEPD NVHNNRDALDKMVGDLHFTCNVNEFANRDAKEGLNVYMYLYTHRTKSNPW PRWTGVMHGDEINYIFGEPLNPNHSYTEEEKDFSRKIMRYWSNFAKYGDP NGATPDVQVWPKHTATGKHYLELGINTTHVGRGPRLRQCAFWKEYLPQLI QATSQTSTNPTSEPCANSGALNGHSLLFLIISFFICILRFENLIQI
Reference
Title: Characterization and functional analysis of two acetylcholinesterase genes in Bradysia odoriphaga Yang et Zhang (Diptera: Sciaridae) Ding Q, Xu X, Wang X, Ullah F, Gao X, Song D Ref: Pestic Biochem Physiol, 174:104807, 2021 : PubMed
Two acetylcholinesterase genes (Boace1 and Boace2) were cloned from Bradysia odoriphaga, a devastating soil pest that mainly damages Chinese chives. The Boace1 encodes BoAChE1 protein consisting of 696 amino acid residues, while Boace2 encodes BoAChE2 containing 638 amino acids. Phylogenetic analysis showed that Boace1 and Boace2 are appeared to be distinct clusters. The gene expression patterns at different development stages and various body parts tissues were examined, and their biological functions were characterized by RNA interference and analog docking prediction. The results showed that both Boace genes were expressed in all developmental stages and examined tissues. The transcript level of Boace2 was significantly higher than Boace1 in all tested samples, and Boace1 was found most abundant in the head while Boace2 was highly expressed in the fat body of B. odoriphaga. The silencing of Boace1 and Boace2 significantly decreased the AChE activity of 36.6% and 14.8% separately, and increased the susceptibility of B. odoriphaga to phoxim, with 60.8% and 44.7% mortality. Besides, overexpression and gene duplication of Boace1 were found in two field resistant populations, and two major mutations, A319S and G400V, were detected in Boace1. Moreover, the docking results revealed that BoAChE1 had a higher affinity towards organophosphorus than BoAChE2. It is concluded that Boace2 is the most abundant ace type in B. odoriphaga, while both Boace play vital roles. Boace1 might play a major neurological function and more likely be the prime target for insecticides, while Boace2 might play some important unidentified roles.
