(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Viridiplantae: NE > Streptophyta: NE > Streptophytina: NE > Embryophyta: NE > Tracheophyta: NE > Euphyllophyta: NE > Spermatophyta: NE > Magnoliophyta: NE > Mesangiospermae: NE > eudicotyledons: NE > Gunneridae: NE > Pentapetalae: NE > asterids: NE > lamiids: NE > Gentianales: NE > Apocynaceae: NE > Rauvolfioideae: NE > Tabernaemontaneae: NE > Tabernaemontaninae: NE > Tabernanthe: NE > Tabernanthe iboga: NE
Molecular evidence
Database
No mutation 1 structure: 6RJ8: Structure of the alpha-beta hydrolase CorS (Coronaridine synthase) from Tabernathe iboga No kinetic
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MANSTANSDEIVFDLHPYIRVFKNGKVERLHDTPYVPPSLEDPATGVSWK DVPISSDVSARVYLPKISEAEKKKLPIFVYFHGAGFCLESAFKSFFHTYV KHVVAETKAVGVSVEYRLAPEHPLPAAYEDCWTALQWVASHVGLDNSSLK NAIDKEPWIINHGDLNKLYLGGDSPGGNIVHNVLIRAGKESLHGGVKIRG AILYYPYFLIRTSKRQSDYMEIDYRGYWKLAYPSAPGGTDNPMINPVAKN APDLAGYGCSRLLVSMVSDETRDITLLYLEALKKSGWKGELEVGDYEAHF FDLFSPENEVGKTWIKRSSDFINKE
Cycloaddition reactions generate chemical complexity in a single step. Here we report the crystal structures of three homologous plant-derived cyclases involved in the biosynthesis of iboga and aspidosperma alkaloids. These enzymes act on the same substrate, named angryline, to generate three distinct scaffolds. Mutational analysis reveals how these highly similar enzymes control regio- and stereo-selectivity.