(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Opisthokonta: NE > Fungi: NE > Dikarya: NE > Ascomycota: NE > saccharomyceta: NE > Pezizomycotina: NE > leotiomyceta: NE > dothideomyceta: NE > Dothideomycetes: NE > Dothideomycetes incertae sedis: NE > Botryosphaeriales: NE > Botryosphaeriaceae: NE > Lasiodiplodia: NE > Lasiodiplodia theobromae: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MHLSSVCLVLSGLLLTNAAPAPVPENGLEKRQSLSSVLSALSGLTEPTAI LSQLEAVEATSTPTSVEQAQEQLEAIYGTTPTNIFENIAQQIADGLSTLT IVQALGFSPSGENSETNSNTREPSTTIYPKKSSSDAPYSITEEELRQAIY IPSDFTYGDKPPVIFVPGTGSYGGISFGSNLRKLLTGVSYADPVWLNVPD ALLRDAQTNGEFVAYAINYISGISGDANVSVVSWSQGGLDTQWAFTYWPS TRALVSDFVPVSPDFHGTVLANVICLNPGAGGVGLGPCAPAVLQQEYNSN FVTALRAAGGADAYVPTTSVFSGFLDEIVQPQSGTGASAYINDARGVGTT NAEVQVVCKGKGPAGGFYTHESLLVNPLTYALLVDALTHDGPGSVDRLDL DTVCSTVVAPGLGLDALLEIEGVNVLAAVNLLTYSDRRLAEPALMSYAA
References
Title: A Novel Lipase from Lasiodiplodia theobromae Efficiently Hydrolyses C8-C10 Methyl Esters for the Preparation of Medium-Chain Triglycerides' Precursors Ng AMJ, Yang R, Zhang H, Xue B, Yew WS, Nguyen GKT Ref: Int J Mol Sci, 22:, 2021 : PubMed
Medium-chain triglycerides (MCTs) are an emerging choice to treat neurodegenerative disorders such as Alzheimer's disease. They are triesters of glycerol and three medium-chain fatty acids, such as capric (C8) and caprylic (C10) acids. The availability of C8-C10 methyl esters (C8-C10 ME) from vegetable oil processes has presented an opportunity to use methyl esters as raw materials for the synthesis of MCTs. However, there are few reports on enzymes that can efficiently hydrolyse C8-C10 ME to industrial specifications. Here, we report the discovery and identification of a novel lipase from Lasiodiplodia theobromae fungus (LTL1), which hydrolyses C8-C10 ME efficiently. LTL1 can perform hydrolysis over pH ranges from 3.0 to 9.0 and maintain thermotolerance up to 70 degreesC. It has high selectivity for monoesters over triesters and displays higher activity over commercially available lipases for C8-C10 ME to achieve 96.17% hydrolysis within 31 h. Structural analysis by protein X-ray crystallography revealed LTL1's well-conserved lipase core domain, together with a partially resolved N-terminal subdomain and an inserted loop, which may suggest its hydrolytic preference for monoesters. In conclusion, our results suggest that LTL1 provides a tractable route towards to production of C8-C10 fatty acids from methyl esters for the synthesis of MCTs.
Lasiodiplodia theobromae (Botryosphaeriaceae, Ascomycota) is a plant pathogen and human opportunist whose pathogenicity is modulated by temperature. The molecular effects of temperature on L. theobromae are mostly unknown, so we used a multi-omics approach to understand how temperature affects the molecular mechanisms of pathogenicity. The genome of L. theobromae LA-SOL3 was sequenced (Illumina MiSeq) and annotated. Furthermore, the transcriptome (Illumina TruSeq) and proteome (Orbitrap LC-MS/MS) of LA-SOL3 grown at 25 degreesC and 37 degreesC were analysed. Proteins related to pathogenicity (plant cell wall degradation, toxin synthesis, mitogen-activated kinases pathway and proteins involved in the velvet complex) were more abundant when the fungus grew at 25 degreesC. At 37 degreesC, proteins related to pathogenicity were less abundant than at 25 degreesC, while proteins related to cell wall organisation were more abundant. On the other hand, virulence factors involved in human pathogenesis, such as the SSD1 virulence protein, were expressed only at 37 degreesC. Taken together, our results showed that this species presents a typical phytopathogenic molecular profile that is compatible with a hemibiotrophic lifestyle. We showed that L. theobromae is equipped with the pathogenesis toolbox that enables it to infect not only plants but also animals.
Lasiodiplodia theobromae Lipase B
