(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Bacteria: NE > Terrabacteria group: NE > Cyanobacteria/Melainabacteria group: NE > Cyanobacteria: NE > Synechococcales: NE > Merismopediaceae: NE > Synechocystis: NE > Synechocystis sp. PCC 6803: NE
Warning: This entry is a compilation of different species or line or strain with more than 90% amino acide identity. You can retrieve all strain data
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) Synechocystis sp. (strain PCC 6803): N, E.
Synechocystis sp. PCC 6803 substr. GT-S: N, E.
Synechocystis sp. PCC 6803 substr. Kazusa: N, E.
Synechocystis sp. PCC 6803 substr. PCC-P: N, E.
Synechocystis sp. PCC 6803 substr. GT-I: N, E.
Synechocystis sp. PCC 6803 substr. PCC-N: N, E.
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MKASFGTERNSFEVQVSGGRLIGSVSEGLGSFLGIPYAAPPVGALRWKPP VADIGWDGARSATSFGNPCTQYLDTIPVITERQRTPSEDCLYLNVWTPAP GDSAKLPVMVWFHGGAFVIGSGSATEFDGANLARHGVVVVTVNYRLGPFG FLSHPELADESSHGASGNYGLLDQLCALQWVAENIAAFGGDPGCVTLFGE SAGGISISCHLASPLSRGLFSRAIMQSGTWFTIPHGLPQASTDPEQAAAN ASKFAASLRCSTQGKVIEELRKRTDGELLAATERRMLFCPVVDGWFLPED PELIFATAKATPVPTILGFNADEGTRFTHTAPGSPEAEELSLRIAVEPTR RMAALMTAPSYCYRFSRVPNTELASRFGAYHGIEVPYVFGNLDARLGYEE EDFALSERMIRYWTTFASSACPDPVGAMTWLRYNKDDENILNLGEG
References
Title: Microevolution in cyanobacteria: re-sequencing a motile substrain of Synechocystis sp. PCC 6803 Trautmann D, Voss B, Wilde A, Al-Babili S, Hess WR Ref: DNA Research, 19:435, 2012 : PubMed
Synechocystis sp. PCC 6803 is a widely used model cyanobacterium for studying photosynthesis, phototaxis, the production of biofuels and many other aspects. Here we present a re-sequencing study of the genome and seven plasmids of one of the most widely used Synechocystis sp. PCC 6803 substrains, the glucose tolerant and motile Moscow or 'PCC-M' strain, revealing considerable evidence for recent microevolution. Seven single nucleotide polymorphisms (SNPs) specifically shared between 'PCC-M' and the 'PCC-N and PCC-P' substrains indicate that 'PCC-M' belongs to the 'PCC' group of motile strains. The identified indels and SNPs in 'PCC-M' are likely to affect glucose tolerance, motility, phage resistance, certain stress responses as well as functions in the primary metabolism, potentially relevant for the synthesis of alkanes. Three SNPs in intergenic regions could affect the promoter activities of two protein-coding genes and one cis-antisense RNA. Two deletions in 'PCC-M' affect parts of clustered regularly interspaced short palindrome repeats-associated spacer-repeat regions on plasmid pSYSA, in one case by an unusual recombination between spacer sequences.
The genome of the unicellular cyanobacterium Synechocystis sp. PCC 6803 consists of a single chromosome and several plasmids of different sizes, and the nucleotide sequences of the chromosome and three small plasmids (5.2 kb, 2.4 kb, and 2.3 kb) have already been sequenced. We newly determined the nucleotide sequences of four large plasmids, which have been identified in our laboratory (pSYSM:120 kb, pSYSX:106 kb, pSYSA:103 kb, and pSYSG:44 kb). Computer-aided analysis was performed to explore the genetic information carried by these plasmids. A total of 397 potential protein-encoding genes were predicted, but little information was obtained about the functional relationship of plasmids to host cell, as a large portion of the predicted genes (77%) were of unknown function. The occurrence of the potential genes on plasmids was divergent, and parA was the only gene common to all four large plasmids. The distribution data of a Cyanobacterium-specific sequence (HIP1: 5'-GCGATCGC-3') suggested that respective plasmids could have originated from different cyanobacterial strains.
