Gene_locus Report for: 9zzzz-AncFT7 Synthetic construct of Renilla-type engineered ancestral luciferase variant (AncFT7) Comment Luciferase from Renilla reniformis (RLuc) catalyzes the degradation of coelenterazine in the presence of molecular oxygen, resulting in the product coelenteramide, carbon dioxide, and the desired photon of light (EC 1.13.12.5). This enzyme belongs to the Haloalkane dehalogenase family II with a different catalytic function (EC 3.8.1.5) Reconstruction of the ancestral enzyme shows it has both hydrolase and monooxygenase activities Relationship (Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> other sequences: N E > artificial sequences: N E > synthetic construct: N E
Molecular evidence | | Database | No mutation1 structure: 7OME: Azacoelenterazine-bound Renilla-type engineered ancestral luciferase variant (AncFT7) No kineticNo SubstrateNo inhibitor | |
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Sequence Graphical view for this peptide sequence: 9zzzz-AncFT7
Colored MSA for Haloalkane_dehalogenase-HLD2 (raw)
MVSASQRTTSTATGDEWWAKCKQVDVLDSEMSYYDSDPGKHKNTVIFLHG
NPTSSYLWRNVIPHVEPLARCLAPDLIGMGKSGKLPNHSYRFVDHYRYLS
AWFDSVNLPEKVTIVCHDWGSGLGFHWCNEHRDRVKGIVHMESVVDVIES
WDEWPDIEEDIALIKSEAGEEMVLKKNFFIERLLPSSIMRKLSEEEMDAY
REPFVEPGESRRPTLTWPREIPLVKGGKPDVIEIVKSYNKWLSTSKDIPK
LFINADPGFFSNAIKKVTKNWPNQKTVTVKGLHFLQEDSPEEIGEAIADF
LNELTK
Legend
This sequence has been compared to family alignement (MSA)
red => minority aminoacid
blue => majority aminoacid
color intensity => conservation rate
title => sequence position(MSA position)aminoacid rate
Catalytic site
Catalytic site in the MSA
MVSASQRTTSTATGDEWWAKCKQVDVLDSEMSYYDSDPGKHKNTVIFLHG
NPTSSYLWRNVIPHVEPLARCLAPDLIGMGKSGKLPNHSYRFVDHYRYLS
AWFDSVNLPEKVTIVCHDWGSGLGFHWCNEHRDRVKGIVHMESVVDVIES
WDEWPDIEEDIALIKSEAGEEMVLKKNFFIERLLPSSIMRKLSEEEMDAY
REPFVEPGESRRPTLTWPREIPLVKGGKPDVIEIVKSYNKWLSTSKDIPK
LFINADPGFFSNAIKKVTKNWPNQKTVTVKGLHFLQEDSPEEIGEAIADF
LNELTK
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