9zzzz-AncHLDRLuc2
Synthetic construct of the common ancestor of haloalkane dehalogenases and Renilla luciferase with fragment transplabntation (Anc-FT) AncFT
Comment
Luciferase from Renilla reniformis (RLuc)<\/A> catalyzes the degradation of coelenterazine in the presence of molecular oxygen, resulting in the product coelenteramide, carbon dioxide, and the desired photon of light (EC 1.13.12.5). This enzyme belongs to the Haloalkane dehalogenase family II with a different catalytic function (EC 3.8.1.5) Reconstruction of the ancestral enzyme shows it has both hydrolase and monooxygenase activities ( Chaloupkova et al.<\/A>) Preprint available of: Engineering Protein Dynamics of Ancestral Luciferase DOI: 10.26434\/chemrxiv.12808295
Relationship
Molecular evidence
Database
Sequence
Peptide
ATGDEWWAKC KQVDVLDSEM SYYDSDPGKH KNTVIFLHGN PTSSYLWRNV IPHVEPLARC LAPDLIGMGK SGKLPNHSYR FVDHYRYLSA WFDSVNLPEK VTIVCHDWGS GLGFHWCNEH RDRVKGIVHM ESVVDVIESW DEWPDIEEDI ALIKSEAGEE MVLKKNFFIE RLLPSSIMRK LSEEEMDAYR EPFVEPGESR RPTLTWPREI PIKGDGPEDV IEIVKSYNKW LSTSKDIPKL FINADPGFFS NAIKKVTKNW PNQKTVTVKG LHFLQEDSPE EIGEAIADFL NELT
References
| Title : Catalytic mechanism for Renilla-type luciferases - Schenkmayerova_2023_Nat.Catal_6_23 |
| Author(s) : Schenkmayerova A , Toul M , Pluskal D , Baatallah R , Gagnot G , Pinto GP , Santana VT , Stuchla M , Neugebauer P , Chaiyen P , Damborsky J , Bednar D , Janin YL , Prokop Z , Marek M |
| Ref : Nature Catalysis , 6 :23 , 2023 |
| Abstract : Schenkmayerova_2023_Nat.Catal_6_23 |
| ESTHER : Schenkmayerova_2023_Nat.Catal_6_23 |
| PubMedSearch : Schenkmayerova_2023_Nat.Catal_6_23 |
| PubMedID: |
| Gene_locus related to this paper: 9zzzz-AncHLDRLuc2 , renre-luc |
| Title : A catalytic mechanism for Renilla-type bioluminescence - Schenkmayerova_2022_Biorxiv__ |
| Author(s) : Schenkmayerova A , Toul M , Pluskal D , Baatallah R , Gagnot G , Pinto GP , Santana VT , Stuchla M , Neugebauer P , Chaiyen P , Damborsky J , Bednar D , Janin YL , Prokop Z , Marek M |
| Ref : Biorxiv , : , 2022 |
| Abstract : Schenkmayerova_2022_Biorxiv__ |
| ESTHER : Schenkmayerova_2022_Biorxiv__ |
| PubMedSearch : Schenkmayerova_2022_Biorxiv__ |
| PubMedID: |
| Gene_locus related to this paper: 9zzzz-AncHLDRLuc2 , renre-luc |
| Title : Light-Emitting Dehalogenases: Reconstruction of Multifunctional Biocatalysts - Chaloupkova_2019_ACS.Catal_9_48103 |
| Author(s) : Chaloupkova, R , Liskova V , Tool M , Markova K , Sebestova E , Hernychova L , Marek M , Pinto GP , Pluskal D , Waterman J , Prokop Z , Damborsky J |
| Ref : ACS Catal , 9 :4810 , 2019 |
| Abstract : Chaloupkova_2019_ACS.Catal_9_48103 |
| ESTHER : Chaloupkova_2019_ACS.Catal_9_48103 |
| PubMedSearch : Chaloupkova_2019_ACS.Catal_9_48103 |
| PubMedID: |
| Gene_locus related to this paper: 9zzzz-AncHLDRLuc2 , 9zzzz-AncHLDRLuc , renre-luc |