Gene_locus Report for: 9zzzz-g3crc6 uncultured organism. Putative uncharacterized protein EstGS
Comment
Classified Family XXVI in Hitch and Clavel (2019) Relationship
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > unclassified sequences: N E > environmental samples: N E > uncultured organism: N E
5_AlphaBeta_hydrolase :
9zzzz-EH82 uncultured organism EH82 EH76 Uncharacterized protein .
6_AlphaBeta_hydrolase :
9zzzz-a0a3g1qtk3 uncultured organism Alpha/beta hydrolases pLP25 Pho25C phosphatase/phytase ,
9zzzz-a0a223he11 uncultured organism Lipase/esterase EstDL33 3-hydroxypalmitic acid methyl ester hydrolase ,
9zzzz-a0a223heg6 uncultured organism Lipase/esterase ELP104 3-hydroxypalmitic acid methyl ester hydrolase ,
9zzzz-a0a223he13 uncultured organism Lipase/esterase ELP96 3-hydroxypalmitic acid methyl ester hydrolase ,
9zzzz-a0a0g3feh8 uncultured organism EH123 Uncharacterized protein ,
9zzzz-a0a0g3feg9 uncultured organism EH103 Uncharacterized protein ,
9bact-t1w006 uncultured bacterium EH72 Esterase ,
9zzzz-a0a0g3fes0 uncultured organism EH47 Uncharacterized protein ,
9zzzz-g3crf7 uncultured organism AB hydrolase-1 domain-containing protein ,
9zzzz-g3cre9 uncultured organism. Putative uncharacterized protein ,
9zzzz-g3cre1 environmental samples uncultured organism Uncharacterized protein ,
9zzzz-g3crf1 environmental samples uncultured organism Putative uncharacterized proteinpLE02 .
A85-Feruloyl-Esterase :
9zzzz-a0a0g3fh10 uncultured organism EH144 Uncharacterized protein ,
9zzzz-a0a0g3fkz4 uncultured organism EH135 Uncharacterized protein ,
9zzzz-a0a0g3fh07 uncultured organism EH134 Uncharacterized protein ,
9zzzz-a0a0g3feq8 uncultured organism EH126 Uncharacterized protein .
A85-IroE-IroD-Fes-Yiel :
uncor-Q99IX4 uncultured organism hypothetical 32.6 kda protein .
ABHD6-Lip :
9zzzz-a0a0g3fh19 uncultured organism EH122 Uncharacterized protein ,
9zzzz-a0a0g3fkz8 uncultured organism EH104 Uncharacterized protein ,
9zzzz-a0a0g3fei1 uncultured organism EH81 Uncharacterized protein ,
9zzzz-m1p1l5 uncultured organism Alpha/beta superfamily hydrolase/acyltransferase .
ABHD11-Acetyl_transferase :
9zzzz-q5g036 uncultured organism putative lipase .
AlphaBeta_hydrolase :
9zzzz-a0a0g3fj48 uncultured organism PLA depolymerase GEN0160 .
Bacterial_lip_FamI.8 :
9zzzz-a0a0g3fh31 uncultured organism EH131 Uncharacterized protein .
BD-FAE :
9bact-a0a1s5qly8 uncultured organism EH108 Uncharacterized protein ,
9bact-a0a1s5qmh4 uncultured organism EH90 Uncharacterized protein ,
9zzzz-KY483647 uncultured organism EH77 Uncharacterized protein ,
9zzzz-g3crf0 uncultured organism AB hydrolase-3 domain-containing protein .
CarbLipBact_2 :
9zzzz-a0a0g3fes4 uncultured organism EH100 Uncharacterized protein .
Carbon-carbon_bond_hydrolase :
9zzzz-g3crc8 environmental samples uncultured organism Uncharacterized protein .
Carb_B_Bacteria :
9zzzz-t1w7u0 uncultured organism Carboxylesterase family ,
9zzzz-t1w4p0 uncultured organism; Sphingomonas sp. Carboxylesterase family ,
9zzzz-t1w943 uncultured organism Carboxylesterase family ,
9zzzz-t1w5l7 uncultured organism Carboxylesterase family ,
9zzzz-a0a0g3feu0 uncultured organism. Uncharacterized protein .
Chlorophyllase :
9zzzz-a0a0g3fh34 uncultured organism EH132 Uncharacterized protein .
Dienelactone_hydrolase :
9zzzz-g3crd8 environmental samples uncultured organism DLH domain-containing protein .
Duf_3089 :
9zzzz-a0a0g3fh03 uncultured organism EH142 Uncharacterized protein .
Epoxide_hydrolase :
9zzzz-c3v8m6 uncultured organism Epoxide hydrolase ,
9zzzz-c3v8m7 uncultured organism Epoxide hydrolase .
Est9X :
9zzzz-a0a0g3fet6 uncultured organism EH71 Uncharacterized protein .
Esterase_phb :
9zzzz-a0a0g3feh5 uncultured organism EH137 Uncharacterized protein .
Glucuronoyl_esterase :
9zzzz-t1wak7 environmental samples uncultured organism Uncharacterized protein .
Homoserine_transacetylase :
9zzzz-a0a0g3fj60 environmental samples uncultured organism, EH28 Uncharacterized protein .
Hormone-sensitive_lipase_like :
9zzzz-XtjR8 uncultured organism hydrolase XtjR8 phthalate-hydrolase from a metagenomic library of lotus pond sludge ,
9zzzz-EstJ6 Uncultured organism clone estj6 EstJ6 phthalate-degrading hydrolase ,
9zzzz-a0a0g3fj39 uncultured organism PLA depolymerase GEN0105 ,
9bact-DMWf18.543 uncultured organism esterase DMWf18-543 ,
9bact-DMWf18.558 uncultured organism esterase DMWf18.558 ,
9zzzz-a0a0g3fh42 uncultured organism EH99 Uncharacterized protein ,
9zzzz-KY483651 uncultured organism EH91 Uncharacterized protein ,
9zzzz-EH87 uncultured organism EH87 Uncharacterized protein ,
9zzzz-a0a0g3fet2 uncultured organism EH79 Uncharacterized protein ,
9zzzz-a0a0g3fj57 Uncultured organism EH52 clone MGS0005 hypothetical protein ,
9zzzz-a0a0g3fh39 Uncultured organism EH43 clone MGS0089 hypothetical protein ,
9zzzz-a0a0g3fl25 Uncultured organism, EH21, clone MGS0091 hypothetical protein ,
9zzzz-q5g037 uncultured organism putative lipase ,
9zzzz-g3crg3 uncultured organism AB hydrolase-3 domain-containing protein ,
9zzzz-g3crf4 uncultured organism Abhydrolase_3 domain-containing protein ,
9zzzz-a0a0g3fej4 uncultured organism. clone MGS0095 EH18 Uncharacterized protein ,
9zzzz-g3crf5 uncultured organism. Putative uncharacterized protein ,
9zzzz-g3crf8 environmental samples uncultured organism Abhydrolase_3 domain-containing protein ,
9zzzz-g3crg1 environmental samples uncultured organism Abhydrolase_3 domain-containing protein ,
9zzzz-g3cre8 environmental samples uncultured organism Abhydrolase_3 domain-containing protein ,
9zzzz-g3crd0 environmental samples uncultured organism Abhydrolase_3 domain-containing protein ,
9zzzz-g3crc9 environmental samples uncultured organism Abhydrolase_3 domain-containing protein ,
9zzzz-g3crf6 environmental samples uncultured organism Abhydrolase_3 domain-containing protein ,
9zzzz-a0a0g3fej0 environmental samples uncultured organism clone MGS0090 EH16 Uncharacterized protein ,
9zzzz-g3crf9 environmental samples uncultured organism Abhydrolase_3 domain-containing protein ,
9zzzz-g3crg2 environmental samples uncultured organism Abhydrolase_3 domain-containing protein ,
9zzzz-g3crd6 environmental samples uncultured organism Abhydrolase_3 domain-containing protein ,
9zzzz-a0a0g3fj64 environmental samples uncultured organism clone MGS0077 EH6 Uncharacterized protein ,
9zzzz-g3cre3 environmental samples uncultured organism Abhydrolase_3 domain-containing protein ,
9zzzz-g3crd2 environmental samples uncultured organism Abhydrolase_3 domain-containing protein pLE02 ,
9zzzz-g3crc7 environmental samples uncultured organism Putative uncharacterized proteinpLE02 ,
9zzzz-a0a0g3feg6 environmental samples uncultured organism GEN0093 EH10 Uncharacterized protein ,
9zzzz-a0a0g3feu6 environmental samples uncultured organism clone MGS0103 EH13 Uncharacterized protein .
Lipase_2 :
uncor-Q91UY0 uncultured organism alkaline lipase (EC 3.1.1.3) ,
9zzzz-g3cre2 environmental samples uncultured organism Abhydrolase_3 domain-containing protein ,
9zzzz-g3cre4 environmental samples uncultured organism Abhydrolase_3 domain-containing protein .
Lipase_3 :
9zzzz-g3cre0 environmental samples uncultured organism Uncharacterized protein EstGH .
Lipase_bact_N_lipase :
9zzzz-a0a0g3fe15 uncultured organism EH75 Uncharacterized protein .
LYsophospholipase_carboxylesterase :
9zzzz-g3cre6 environmental samples uncultured organism Abhydrolase_2 domain-containing protein .
Monoglyceridelipase_lysophospholip :
9zzzz-d8vn29 uncultured organism Hydrolase alpha/beta fold family protein .
PGAP1 :
9zzzz-a0a0g3fj44 uncultured organism EH145 Uncharacterized protein
Molecular evidence
Database
No mutation No structure No kinetic No Substrate No inhibitor
Sequence
Graphical view for this peptide sequence: 9zzzz-g3crc6 Colored MSA for Chlorophyllase (raw)
MSLHPSPVRLVLVSIALATLVAACSDSGSGSPPATSEDFLAPGPFAVSST
SVSLIDTSRATPPAGTSPGSSTRVLPTDVYFPAQGGEPGALVPGAALDSD
GGPYPLILFAHGLFGARTNFQATLVHLASHGYVVAAADFPETNFGTIVAG
TANIADLINQPGDLSFLIDVLTTSPTPALEPIAAAVDGERIGVLGHSFGG
ATSILLAYGGSVADPRIDAVVTAAPFSCFAGAALFSGAREVPFLDLHGTS
DGIVDVAWTEDLFPRLPTPRYRGDIVGGDHLGFLSDPTVPSSFRDVQAYA
LFSTASGSFAAQFAALSEALATRVPAADVSLCASRPIFPDPTTPRDPFIG
VARQVEITDVATTAFFAAYVKDDGSARAYLADGFAAFAPDVRYRAEQ
Legend
This sequence has been compared to family alignement (MSA)
red => minority aminoacid
blue => majority aminoacid
color intensity => conservation rate
title => sequence position(MSA position)aminoacid rate
Catalytic site
Catalytic site in the MSA
M S L H P S P V R L V L V S I A L A T L V A A C S D S G S G S P P A T S E D F L A P G P F A V S S T S V S L I D T S R A T P P A G T S P G S S T R V L P T D V Y F P A Q G G E P G A L V P G A A L D S D G G P Y P L I L F A H G L F G A R T N F Q A T L V H L A S H G Y V V A A A D F P E T N F G T I V A G T A N I A D L I N Q P G D L S F L I D V L T T S P T P A L E P I A A A V D G E R I G V L G H S F G G A T S I L L A Y G G S V A D P R I D A V V T A A P F S C F A G A A L F S G A R E V P F L D L H G T S D G I V D V A W T E D L F P R L P T P R Y R G D I V G G D H L G F L S D P T V P S S F R D V Q A Y A L F S T A S G S F A A Q F A A L S E A L A T R V P A A D V S L C A S R P I F P D P T T P R D P F I G V A R Q V E I T D V A T T A F F A A Y V K D D G S A R A Y L A D G F A A F A P D V R Y R A E Q
Reference
Title: Identification of novel lipolytic genes and gene families by screening of metagenomic libraries derived from soil samples of the German Biodiversity Exploratories
Nacke H , Will C , Herzog S , Nowka B , Engelhaupt M , Daniel R
Ref: FEMS Microbiol Ecol, 78 :188, 2011 : PubMed Abstract ESTHER: Nacke_2011_FEMS.Microbiol.Ecol_78_188 PubMedSearch: Nacke 2011 FEMS.Microbiol.Ecol 78 188 PubMedID: 21395625 Gene_locus related to this paper: 9zzzz-g3crf3 ,
9zzzz-g3crg0 ,
9zzzz-g3crd1 ,
9zzzz-g3crg3 ,
9zzzz-g3crf0 ,
9zzzz-g3crf7 ,
9zzzz-g3crf4 ,
9zzzz-g3cre9 ,
9zzzz-g3crf5 ,
9zzzz-g3crf8 ,
9zzzz-g3crc8 ,
9zzzz-g3crg1 ,
9zzzz-g3cre0 ,
9zzzz-g3cre6 ,
9zzzz-g3cre1 ,
9zzzz-g3cre2 ,
9zzzz-g3cre8 ,
9zzzz-g3cre4 ,
9zzzz-g3crd0 ,
9zzzz-g3crc9 ,
9zzzz-g3crf6 ,
9zzzz-g3crf9 ,
9zzzz-g3crg2 ,
9zzzz-g3crd8 ,
9zzzz-g3crd6 ,
9zzzz-g3cre3 ,
9zzzz-g3crd2 ,
9zzzz-g3crf1 ,
9zzzz-g3crc7 ,
9zzzz-g3crc6 Abstract
Microbial metagenomes derived from soils are rich sources for the discovery of novel genes and biocatalysts. Fourteen environmental plasmid and seven fosmid libraries obtained from 10 German forest soils (A horizons) and six grassland soils (A and B horizons) were screened for genes conferring lipolytic activity. The libraries comprised approximately 29.3 Gb of cloned soil DNA. Partial activity-based screening of the constructed libraries resulted in the identification of 37 unique lipolytic clones. The amino acid sequences of the 37 corresponding lipolytic gene products shared 29-90% identity to other lipolytic enzymes, which were mainly uncharacterized or derived from uncultured microorganisms. Multiple sequence alignments and phylogenetic tree analysis revealed that 35 of the predicted proteins were new members of known families of lipolytic enzymes. The remaining two gene products represent two putatively new families. In addition, sequence analysis indicated that two genes encode true lipases, whereas the other genes encode esterases. The determination of substrate specificity and chain-length selectivity using different triacylglycerides and p-nitrophenyl esters of fatty acids as substrates supported the classification of the esterases.
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