(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Bacteria: NE > Proteobacteria: NE > Acidithiobacillia: NE > Acidithiobacillales: NE > Acidithiobacillaceae: NE > Acidithiobacillus: NE > Acidithiobacillus ferrooxidans: NE
Warning: This entry is a compilation of different species or line or strain with more than 90% amino acid identity. You can retrieve all strain data
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) Acidithiobacillus ferrooxidans ATCC 23270: N, E.
Acidithiobacillus ferrooxidans ATCC 53993: N, E.
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MPVEIPADSVGLVTPQTVTFSAPLELECGRSLPGYTLTYETYGTLNAQRS NAILLCHALSGDHHAAGYHSMDDRKPGWWEHLLGPGKAMDTERFFFVCAN FIGSCKGSTGPASTNPETGAPWGLDFPMVTVRDWVKTQAELADYLGIEQW AAVVGGSLGGMQVMQWSMDYPERLRHAVVIAAAPKLTAQNIAFNEVCRQA IMTDPEFYNGRYYAHNTKPRRGLSLARMIGHITYLSDNAMRTKFGRDTRG GKAFSFGFDVDFEVESYLRYQGSSFVERFDANSYLYITKALDYFDPASTW GGNLAEAFASTRANFLVISFSSDWRFSPERSREIVQALYTCNRDVSYAEI EAEHGHDSFLMPIPQYVAVLSTYLGRVAEEIGA
BACKGROUND: Acidithiobacillus ferrooxidans is a major participant in consortia of microorganisms used for the industrial recovery of copper (bioleaching or biomining). It is a chemolithoautrophic, gamma-proteobacterium using energy from the oxidation of iron- and sulfur-containing minerals for growth. It thrives at extremely low pH (pH 1-2) and fixes both carbon and nitrogen from the atmosphere. It solubilizes copper and other metals from rocks and plays an important role in nutrient and metal biogeochemical cycling in acid environments. The lack of a well-developed system for genetic manipulation has prevented thorough exploration of its physiology. Also, confusion has been caused by prior metabolic models constructed based upon the examination of multiple, and sometimes distantly related, strains of the microorganism. RESULTS: The genome of the type strain A. ferrooxidans ATCC 23270 was sequenced and annotated to identify general features and provide a framework for in silico metabolic reconstruction. Earlier models of iron and sulfur oxidation, biofilm formation, quorum sensing, inorganic ion uptake, and amino acid metabolism are confirmed and extended. Initial models are presented for central carbon metabolism, anaerobic metabolism (including sulfur reduction, hydrogen metabolism and nitrogen fixation), stress responses, DNA repair, and metal and toxic compound fluxes. CONCLUSION: Bioinformatics analysis provides a valuable platform for gene discovery and functional prediction that helps explain the activity of A. ferrooxidans in industrial bioleaching and its role as a primary producer in acidic environments. An analysis of the genome of the type strain provides a coherent view of its gene content and metabolic potential.
