(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Bacteria: NE > Proteobacteria: NE > Alphaproteobacteria: NE > Rhizobiales: NE > Rhizobiaceae: NE > Rhizobium/Agrobacterium group: NE > Agrobacterium: NE > Agrobacterium tumefaciens complex: NE > Agrobacterium tumefaciens: NE
Warning: This entry is a compilation of different species or line or strain with more than 90% amino acide identity. You can retrieve all strain data
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) Agrobacterium tumefaciens str. C58: N, E.
Agrobacterium fabrum str. C58: N, E.
MSAISRHIIDRGDGAAVELFQAQTAFPPKGAILFVHGNQGGLLLGAKEAV
DNGMLLRFSSRLNVTAAAVSQPGFGASEGPTDFCGPTTQQAIIAALGFLQ
KQSLIDPERIILYGNSRGAVASAMVATQMPDLRAVILSSGVYDLKRAYQS
SPRGLQLAIEKEAGLTNTAFLDRSALFHSHKIRAETLLLHGKHDDRAPVD
QAERFANAISEAGLSVDLQTFECGHRIAGELTAPVLRNFLTRILAPAATF
H
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MSAISRHIIDRGDGAAVELFQAQTAFPPKGAILFVHGNQGGLLLGAKEAV DNGMLLRFSSRLNVTAAAVSQPGFGASEGPTDFCGPTTQQAIIAALGFLQ KQSLIDPERIILYGNSRGAVASAMVATQMPDLRAVILSSGVYDLKRAYQS SPRGLQLAIEKEAGLTNTAFLDRSALFHSHKIRAETLLLHGKHDDRAPVD QAERFANAISEAGLSVDLQTFECGHRIAGELTAPVLRNFLTRILAPAATF H
Agrobacterium tumefaciens is a plant pathogen capable of transferring a defined segment of DNA to a host plant, generating a gall tumor. Replacing the transferred tumor-inducing genes with exogenous DNA allows the introduction of any desired gene into the plant. Thus, A. tumefaciens has been critical for the development of modern plant genetics and agricultural biotechnology. Here we describe the genome of A. tumefaciens strain C58, which has an unusual structure consisting of one circular and one linear chromosome. We discuss genome architecture and evolution and additional genes potentially involved in virulence and metabolic parasitism of host plants.
The 5.67-megabase genome of the plant pathogen Agrobacterium tumefaciens C58 consists of a circular chromosome, a linear chromosome, and two plasmids. Extensive orthology and nucleotide colinearity between the genomes of A. tumefaciens and the plant symbiont Sinorhizobium meliloti suggest a recent evolutionary divergence. Their similarities include metabolic, transport, and regulatory systems that promote survival in the highly competitive rhizosphere; differences are apparent in their genome structure and virulence gene complement. Availability of the A. tumefaciens sequence will facilitate investigations into the molecular basis of pathogenesis and the evolutionary divergence of pathogenic and symbiotic lifestyles.
Title: A region of the Agrobacterium tumefaciens chromosome containing genes required for virulence and attachment to host cells Matthysse AG, Yarnall H, Boles SB, McMahan S Ref: Biochimica & Biophysica Acta, 1490:208, 2000 : PubMed
A 29 kb region of the circular chromosome of Agrobacterium tumefaciens containing genes required for bacterial attachment to host cells and virulence has been sequenced. Transposon mutants in many of the genes have been obtained. The mutants can be divided into two groups: those which can be complemented by conditioned medium and those whose phenotype is unaffected by conditioned medium. The first group includes mutants in genes with homology to ABC transporters, one possible transcriptional regulator, and some closely linked genes immediately downstream. The second group includes mutants in two possible transcriptional regulators, one ATPase, and a number of biosynthetic genes including a transacetylase required for the formation of an acetylated capsular polysaccharide. There are also several genes with no homology to genes of identified function. The presence of such a large number of genes required for attachment to host cells suggests that the ability of A. tumefaciens to bind to plant cells may play an important role in the life of these bacteria.
Agrobacterium tumefaciens (strain C58 / ATCC 33970) Esterase D