(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Bacteria: NE > Terrabacteria group: NE > Actinobacteria [phylum]: NE > Actinobacteria [class]: NE > Pseudonocardiales: NE > Pseudonocardiaceae: NE > Amycolatopsis: NE > Amycolatopsis mediterranei: NE
Warning: This entry is a compilation of different species or line or strain with more than 90% amino acide identity. You can retrieve all strain data
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) Amycolatopsis mediterranei U32: N, E.
Amycolatopsis rifamycinica: N, E.
Amycolatopsis mediterranei S699: N, E.
Amycolatopsis tolypomycina: N, E.
Amycolatopsis pretoriensis: N, E.
Amycolatopsis thailandensis: N, E.
Amycolatopsis sp. GLM-1: N, E.
Amycolatopsis balhimycina DSM 5908: N, E.
Amycolatopsis vancoresmycina DSM 44592: N, E.
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MRRLFTAGAVLLALAGLAPAAAAAPDIKAQLQRIPGLTIVSEDAAPAGFR FFKLTYTQPADHRHPGAGTFQQRFTLLHRDFAAPTVAFTSGYNVSGSPNR SEPTQIVDGNQLSMEYRYFTPSRPEPENWAKQLTIWQAAADEHRAVQAFK AIYPGKWLATGGSKGGMTATYFRRFFPDDVDATIPYVAPNDVIDPIDVYN RFLSRVGDDPACRDALKAIQRDALKRRDELGPIAAADAAQRGLTFSIVGS ADKSLEISVIDSYFAFWQYQKQADCATVPPAGAPAADVYAWYEKVESLNT YSDQDLAPYIPYYYQAAVQLGSPEAYDGYLRDLLRYPGADQPKTFVPASI RLPRFDYLAMPDIDFWVKSRGTRLLFVYGANDPWGAEPFELGFGSRDSYR YYVQGGNHGSKIAQLAPPDAAAATATVRRWAGLPPVAPLAARSAPAGFPD FDTDLTPRARL
Amycolatopsis mediterranei is used for industry-scale production of rifamycin, which plays a vital role in antimycobacterial therapy. As the first sequenced genome of the genus Amycolatopsis, the chromosome of strain U32 comprising 10,236,715 base pairs, is one of the largest prokaryotic genomes ever sequenced so far. Unlike the linear topology found in streptomycetes, this chromosome is circular, particularly similar to that of Saccharopolyspora erythraea and Nocardia farcinica, representing their close relationship in phylogeny and taxonomy. Although the predicted 9,228 protein-coding genes in the A. mediterranei genome shared the greatest number of orthologs with those of S. erythraea, it was unexpectedly followed by Streptomyces coelicolor rather than N. farcinica, indicating the distinct metabolic characteristics evolved via adaptation to diverse ecological niches. Besides a core region analogous to that common in streptomycetes, a novel 'quasi-core' with typical core characteristics is defined within the non-core region, where 21 out of the total 26 gene clusters for secondary metabolite production are located. The rifamycin biosynthesis gene cluster located in the core encodes a cytochrome P450 enzyme essential for the conversion of rifamycin SV to B, revealed by comparing to the highly homologous cluster of the rifamycin B-producing strain S699 and further confirmed by genetic complementation. The genomic information of A. mediterranei demonstrates a metabolic network orchestrated not only for extensive utilization of various carbon sources and inorganic nitrogen compounds but also for effective funneling of metabolic intermediates into the secondary antibiotic synthesis process under the control of a seemingly complex regulatory mechanism.
Amycolatopsis mediterranei (Nocardia mediterranei) rifr Thioesterase domain