(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Bacteria: NE > Terrabacteria group: NE > Firmicutes: NE > Bacilli: NE > Bacillales: NE > Bacillaceae: NE > Bacillus: NE > Bacillus subtilis group: NE > Bacillus amyloliquefaciens group: NE > Bacillus amyloliquefaciens: NE
Warning: This entry is a compilation of different species or line or strain with more than 90% amino acid identity. You can retrieve all strain data
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) Bacillus amyloliquefaciens DSM 7: N, E.
Bacillus amyloliquefaciens LL3: N, E.
Bacillus amyloliquefaciens EGD-AQ14: N, E.
Bacillus amyloliquefaciens IT-45: N, E.
Bacillus amyloliquefaciens Y2: N, E.
Bacillus amyloliquefaciens TA208: N, E.
Bacillus amyloliquefaciens EBL11: N, E.
Bacillus amyloliquefaciens LFB112: N, E.
Bacillus amyloliquefaciens XH7: N, E.
Bacillus amyloliquefaciens UASWS BA1: N, E.
Bacillus amyloliquefaciens CC178: N, E.
Bacillus amyloliquefaciens UMAF6639: N, E.
Bacillus amyloliquefaciens KHG19: N, E.
Bacillus amyloliquefaciens FZB42: N, E.
Bacillus velezensis FZB42: N, E.
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MKAAEIDQNKPLTEYGFDSISSIQLLKKLEDGDSRISLEALQKCSTLQEI GMLLKTNQPQETGRNFPELVKLNEREKGRPVFWFHGGTGGVEAYQPFAKK SQRPFYGIQARGLTGKEPLQGIEETAASYKRIIQAVQPKGPYDLGGYSLG GMLAYETARLLQEEGHTVKSAVMIDTPYSEKWKERKPSVKSVMLQTINTM LTAYLKPENLEEALISREDITVQAEEEEVLKELIGLAKKRGLPKSEEAIR MQLEQMMKTQLAYGMEEYDMKPLPHPEALQCYYFRNKSGLFLGDLERYLT AEEEQIPLDHDAYWKEWEHQMKQFHLLDVHSSNHVTMLSEPKPQNAIKAF CEKLYANKGTISANFLKSFRKKLDETNELVKR
Bacillus amyloliquefaciens FZB42 is a Gram-positive, plant-associated bacterium, which stimulates plant growth and produces secondary metabolites that suppress soil-borne plant pathogens. Its 3,918-kb genome, containing an estimated 3,693 protein-coding sequences, lacks extended phage insertions, which occur ubiquitously in the closely related Bacillus subtilis 168 genome. The B. amyloliquefaciens FZB42 genome reveals an unexpected potential to produce secondary metabolites, including the polyketides bacillaene and difficidin. More than 8.5% of the genome is devoted to synthesizing antibiotics and siderophores by pathways not involving ribosomes. Besides five gene clusters, known from B. subtilis to mediate nonribosomal synthesis of secondary metabolites, we identified four giant gene clusters absent in B. subtilis 168. The pks2 gene cluster encodes the components to synthesize the macrolactin core skeleton.
Although bacterial polyketides are of considerable biomedical interest, the molecular biology of polyketide biosynthesis in Bacillus spp., one of the richest bacterial sources of bioactive natural products, remains largely unexplored. Here we assign for the first time complete polyketide synthase (PKS) gene clusters to Bacillus antibiotics. Three giant modular PKS systems of the trans-acyltransferase type were identified in Bacillus amyloliquefaciens FZB 42. One of them, pks1, is an ortholog of the pksX operon with a previously unknown function in the sequenced model strain Bacillus subtilis 168, while the pks2 and pks3 clusters are novel gene clusters. Cassette mutagenesis combined with advanced mass spectrometric techniques such as matrix-assisted laser desorption ionization-time of flight mass spectrometry and liquid chromatography-electrospray ionization mass spectrometry revealed that the pks1 (bae) and pks3 (dif) gene clusters encode the biosynthesis of the polyene antibiotics bacillaene and difficidin or oxydifficidin, respectively. In addition, B. subtilis OKB105 (pheA sfp(0)), a transformant of the B. subtilis 168 derivative JH642, was shown to produce bacillaene, demonstrating that the pksX gene cluster directs the synthesis of that polyketide. The GenBank accession numbers for gene clusters pks1(bae), pks2, and pks3(dif) are AJ 634060.2, AJ 6340601.2, and AJ 6340602.2, respectively.
