canar-LipB

 
Candida antarctica (Trichosporon oryzae) (yeast) Lipase B CALB, CALipB ,Novozyme 435

Comment
Other strains: Pseudozyma antarctica (strain T-34) (Yeast) (Candida antarctica); Pseudozyma aphidis DSM 70725 ; Through structure homology this protein is close to other fungus Lipase_3 but it is far from all other sequence in ESTHER. Novozyme 435 (Novozyme-435 N435, N-435, Novozym 435 Novozym435 Novozyme435) is CAL-B immobilized onto a macroporous acrylic polymer resin (Lewatit VP OC 1600, Bayer) by Novozyme. Fermenta Biotech BEX 10000 ; promiscuous activity 68/96 substrates Martinez-Martinez et al. 2018


Relationship
Family Canar_LipB
Block L
Candida antarctica position in NCBI Life Tree :
N link to NCBI taxonomic web page and E link to ESTHER gene locus found in this strain.
> cellular organisms: N E > Eukaryota: N E > Opisthokonta: N E > Fungi: N E > Dikarya: N E > Basidiomycota: N E > Ustilaginomycotina: N E > Ustilaginomycetes: N E > Ustilaginales: N E > Ustilaginaceae: N E > Moesziomyces: N E > Moesziomyces antarcticus: N E
Warning: This entry is a compilation of different species or line or strain with more than 90% amino acide identity. You can retrieve all strain data


Molecular evidence
Database
No mutation
25 structures (e.g. : 1LBS, 1LBT, 1TCA... more)
No kinetic

Substrate: alpha-Methylbenzyl-butyrate , 2-Ethylhexyl-acetate , Isobutyl-propionate , Ethyl-2-ethylhexanoate , Ethyl-2-methylbutyrate , Ethyl-Flurbiprofen , Ethyl-2-phenylpropionate , Pentadecalactone , Diethyl-adipate , Polybutylene-succinate , T-80 , DiFMU-octanoate , Octylglucoside , Methyl-3-hydroxypentanoate , Ethylbenzoate , Naproxen-Ethyl-Ester , Naproxen , Ketoprofen-Glycerol-Ester , Ketoprofen-Vinyl-Ester , Ketoprofen-Butyl-Ester , Ketoprofen-Ethyl-Ester , Phenylacetate , Tributyrin , 1,3-Diolein , Trihexanoin , Geranyl-acetate ,
Inhibitor: 6J1T-B7U , Rh-pNP , ArM-Rhubidium-cofactor-8 , Phosphonate-MSW , 4-methylumbelliferyl-hexylphosphonate , N-Hexylphosphonate-Ethyl-Ester ,
>3 Genbank links 1 more: Z30645, DF196778, AWNI01000010
>3 UniProt links 1 more: P41365, M9MDK9, W3VNS6
1 Ncbi-nid : 515791
1 Ncbi-pid : 515792
>3 Structure links 22 more: 1LBS, 1LBT, 1TCA
>3 UniProtTrembl links 1 more: P41365, M9MDK9, W3VNS6
>3 Interpro links 1 more: P41365, M9MDK9, W3VNS6
>3 Prodom links 1 more: P41365, M9MDK9, W3VNS6
>3 Pfam links 1 more: P41365, M9MDK9, W3VNS6
>3 PIRSF links 1 more: P41365, M9MDK9, W3VNS6
>3 SUPERFAM links 1 more: P41365, M9MDK9, W3VNS6
>3 QuickSwissBlast links 1 more: P41365, M9MDK9, W3VNS6
 
Sequence
Graphical view for this peptide sequence: canar-LipB
Colored MSA for Canar_LipB (raw)
MKLLSLTGVAGVLATCVAATPLVKRLPSGSDPAFSQPKSVLDAGLTCQGA
SPSSVSKPILLVPGTGTTGPQSFDSNWIPLSTQLGYTPCWISPPPFMLND
TQVNTEYMVNAITALYAGSGNNKLPVLTWSQGGLVAQWGLTFFPSIRSKV
DRLMAFAPDYKGTVLAGPLDALAVSAPSVWQQTTGSALTTALRNAGGLTQ
IVPTTNLYSATDEIVQPQVSNSPLDSSYLFNGKNVQAQAVCGPLFVIDHA
GSLTSQFSYVVGRSALRSTTGQARSADYGITDCNPLPANDLTPEQKVAAA
ALLAPAAAAIVAGPKQNCEPDLMPYARPFAVGKRTCSGIVTP
Legend This sequence has been compared to family alignement (MSA)
red => minority aminoacid
blue => majority aminoacid
color intensity => conservation rate
title => sequence position(MSA position)aminoacid rate
Catalytic site
Catalytic site in the MSA

MKLLSLTGVAGVLATCVAATPLVKRLPSGSDPAFSQPKSVLDAGLTCQGA
SPSSVSKPILLVPGTGTTGPQSFDSNWIPLSTQLGYTPCWISPPPFMLND
TQVNTEYMVNAITALYAGSGNNKLPVLTWSQGGLVAQWGLTFFPSIRSKV
DRLMAFAPDYKGTVLAGPLDALAVSAPSVWQQTTGSALTTALRNAGGLTQ
IVPTTNLYSATDEIVQPQVSNSPLDSSYLFNGKNVQAQAVCGPLFVIDHA
GSLTSQFSYVVGRSALRSTTGQARSADYGITDCNPLPANDLTPEQKVAAA
ALLAPAAAAIVAGPKQNCEPDLMPYARPFAVGKRTCSGIVTP

Graphical view for this nucleotide DNA sequence (1062 bp): canar-LipB



References
50 more
    Title: Evidence for a high pK(a) of an aspartic acid residue in the active site of CALB by a fully atomistic multiscale approach
    Capone M, Zanetti-Polzi L, Leonzi I, Spreti N, Daidone I
    Ref: J Biomol Struct Dyn, :1, 2022 : PubMed

            

    Title: Enhanced biodegradation of waste poly(ethylene terephthalate) using a reinforced plastic degrading enzyme complex
    Hwang DH, Lee ME, Cho BH, Oh JW, You SK, Ko YJ, Hyeon JE, Han SO
    Ref: Sci Total Environ, :156890, 2022 : PubMed

            

    Title: Principles of lipid-enzyme interactions in the limbus region of the catalytic site of Candida antarctica Lipase B
    Silvestrini L, Cianci M
    Ref: Int J Biol Macromol, :, 2020 : PubMed

            


Other Papers


Send your questions or comments to :
Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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