Gene_Locus Report

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Gene_locus Report for: castro-TS

Catharanthus roseus (Madagascar periwinkle) hydrolase 2, 2-hydroxyisoflavanone dehydratase Catharanthine Synthase CS

Comment
Component of iboga and aspidosperma monoterpenoid indole alkaloids (MIAs, e.g. tabersonine and catharanthine) biosynthesis pathway from 19E-geissoschizine. Catalyzes the conversion of O-acetylstemmadenine (OAS) to tabersonine, a precursor of vindoline.


Relationship
Family|Plant_carboxylesterase
Block| H
Position in NCBI Life Tree|Catharanthus roseus
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms: N E > Eukaryota: N E > Viridiplantae: N E > Streptophyta: N E > Streptophytina: N E > Embryophyta: N E > Tracheophyta: N E > Euphyllophyta: N E > Spermatophyta: N E > Magnoliophyta: N E > Mesangiospermae: N E > eudicotyledons: N E > Gunneridae: N E > Pentapetalae: N E > asterids: N E > lamiids: N E > Gentianales: N E > Apocynaceae: N E > Rauvolfioideae: N E > Vinceae: N E > Catharanthinae: N E > Catharanthus: N E > Catharanthus roseus: N E


Molecular evidence
Database
No mutation
1 structure:
6RS4: Structure of tabersonine synthase - an alpha-beta hydrolase from Catharanthus roseus
No kinetic





No Substrate
No inhibitor
2 Genbank : AVM85921, MF770513
1 UniProt : A0A2P1GIW3
1 Structure : 6RS4
1 UniProt : A0A2P1GIW3
1 Interpro : A0A2P1GIW3
1 Prodom : A0A2P1GIW3
1 Pfam : A0A2P1GIW3
1 PIRSF : A0A2P1GIW3
1 SUPERFAM : A0A2P1GIW3
Sequence
Graphical view for this peptide sequence: castro-TS
Colored MSA for Plant_carboxylesterase (raw)
MGSSDETIFDLPPYIKVFKDGRVERLHSSPYVPPSLNDPETGGVSWKDVP
ISSVVSARIYLPKINNHDEKLPIIVYFHGAGFCLESAFKSFFHTYVKHFV
AEAKAIAVSVEFRLAPENHLPAAYEDCWEALQWVASHVGLDISSLKTCID
KDPWIINYADFDRLYLWGDSTGANIVHNTLIRSGKEKLNGGKVKILGAIL
YYPYFLIRTSSKQSDYMENEYRSYWKLAYPDAPGGNDNPMINPTAENAPD
LAGYGCSRLLISMVADEARDITLLYIDALEKSGWKGELDVADFDKQYFEL
FEMETEVAKNMLRRLASFIK
Legend This sequence has been compared to family alignement (MSA)
red => minority aminoacid
blue => majority aminoacid
color intensity => conservation rate
title => sequence position(MSA position)aminoacid rate
Catalytic site
Catalytic site in the MSA

MGSSDETIFDLPPYIKVFKDGRVERLHSSPYVPPSLNDPETGGVSWKDVP
ISSVVSARIYLPKINNHDEKLPIIVYFHGAGFCLESAFKSFFHTYVKHFV
AEAKAIAVSVEFRLAPENHLPAAYEDCWEALQWVASHVGLDISSLKTCID
KDPWIINYADFDRLYLWGDSTGANIVHNTLIRSGKEKLNGGKVKILGAIL
YYPYFLIRTSSKQSDYMENEYRSYWKLAYPDAPGGNDNPMINPTAENAPD
LAGYGCSRLLISMVADEARDITLLYIDALEKSGWKGELDVADFDKQYFEL
FEMETEVAKNMLRRLASFIK


References
    Title: Structural basis of cycloaddition in biosynthesis of iboga and aspidosperma alkaloids
    Caputi L, Franke J, Bussey K, Farrow SC, Vieira IJC, Stevenson CEM, Lawson DM, O'Connor SE
    Ref: Nat Chemical Biology, 16:383, 2020 : PubMed

            

    Title: Missing enzymes in the biosynthesis of the anticancer drug vinblastine in Madagascar periwinkle
    Caputi L, Franke J, Farrow SC, Chung K, Payne RME, Nguyen TD, Dang TT, Soares Teto Carqueijeiro I, Koudounas K and O'Connor SE <5 more author(s)>
    Ref: Science, 360:1235, 2018 : PubMed

            

    Title: Solution of the multistep pathway for assembly of corynanthean, strychnos, iboga, and aspidosperma monoterpenoid indole alkaloids from 19E-geissoschizine
    Qu Y, Easson M, Simionescu R, Hajicek J, Thamm AMK, Salim V, De Luca V
    Ref: Proc Natl Acad Sci U S A, 115:3180, 2018 : PubMed

            


Other Papers


Send your questions or comments to :
Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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