(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Bacteria: NE > Terrabacteria group: NE > Actinobacteria [phylum]: NE > Actinobacteria [class]: NE > Corynebacteriales: NE > Corynebacteriaceae: NE > Corynebacterium: NE > Corynebacterium glutamicum: NE
Warning: This entry is a compilation of different species or line or strain with more than 90% amino acide identity. You can retrieve all strain data
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) Corynebacterium glutamicum ATCC 14067: N, E.
Corynebacterium glutamicum R: N, E.
Corynebacterium glutamicum ATCC 13032: N, E.
Corynebacterium glutamicum SCgG1: N, E.
Corynebacterium glutamicum Z188: N, E.
Corynebacterium glutamicum S9114: N, E.
Corynebacterium glutamicum K051: N, E.
Corynebacterium glutamicum SCgG2: N, E.
Corynebacterium glutamicum MB001: N, E.
[Brevibacterium] flavum: N, E.
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MAENLNKHLSKLSKRGPHRVLVGDMNYAGIPGKIYTPAEGDGIPGVAFGH DWMKSIKYYHQTLRHLASWGIAVAAPDTENGFMPDHKGFASDLESSIQIL GGVKLGSGNVTVNPACLGVVGHGMGAGAAVLSAANRDLVRAVGAIYPAKT SPSAIDAAFAVKAPGLVIGSSSLGLFESGEPAKLAANWAGDVCYRESEKG NQQGFSEDTMFKLVAGIGSPQTGAQETVRGLLTGFLLHQLAGEKKYKAFS EPDAEAKKVVSYFGQELQEHAFPKDTSPFAFLNEK
The complete genomic sequence of Corynebacterium glutamicum ATCC 13032, well-known in industry for the production of amino acids, e.g. of L-glutamate and L-lysine was determined. The C. glutamicum genome was found to consist of a single circular chromosome comprising 3282708 base pairs. Several DNA regions of unusual composition were identified that were potentially acquired by horizontal gene transfer, e.g. a segment of DNA from C. diphtheriae and a prophage-containing region. After automated and manual annotation, 3002 protein-coding genes have been identified, and to 2489 of these, functions were assigned by homologies to known proteins. These analyses confirm the taxonomic position of C. glutamicum as related to Mycobacteria and show a broad metabolic diversity as expected for a bacterium living in the soil. As an example for biotechnological application the complete genome sequence was used to reconstruct the metabolic flow of carbon into a number of industrially important products derived from the amino acid L-aspartate.
Title: The alanine racemase gene alr is an alternative to antibiotic resistance genes in cloning systems for industrial Corynebacterium glutamicum strains Tauch A, Gotker S, Puhler A, Kalinowski J, Thierbach G Ref: J Biotechnol, 99:79, 2002 : PubMed
The potential of the alanine racemase gene alr from Corynebacterium glutamicum ATCC 13032 to substitute for antibiotic resistance determinants in cloning systems has been investigated. The alr gene was identified by a PCR technique and its nucleotide sequence was determined. The deduced protein revealed the highest amino acid sequence similarity to the Alr protein from Mycobacterium smegmatis with 45% identical and 58% similar amino acids. A defined alr deletion mutant of C. glutamicum displayed a strict dependence on the presence of D-alanine for growth on complex and minimal medium. The alr gene was placed on a novel C. glutamicum vector which is completely free of antibiotic resistance genes. In vivo complementation of the chromosomal alr deletion with alr-carrying vectors permitted growth of the mutant strain in the absence of external D-alanine and provided strong selective pressure to maintain the plasmid. The alr gene enabled the selection of C. glutamicum transformants with a similar efficiency as the tetracycline resistance gene tetA(33). These data provided experimental evidence that the alr gene can be applied as an alternative selection marker to antibiotic resistance genes in industrial C. glutamicum strains. In an application example, the novel deltaalr host-alr(+) vector-system for C. glutamicum was used to overproduce the vitamin D-pantothenic acid.
Corynebacterium glutamicum (Brevibacterium flavum), hypothetical protein cgl0343