(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Bacteria: NE > Terrabacteria group: NE > Actinobacteria [phylum]: NE > Actinobacteria [class]: NE > Corynebacteriales: NE > Corynebacteriaceae: NE > Corynebacterium: NE > Corynebacterium glutamicum: NE
Warning: This entry is a compilation of different species or line or strain with more than 90% amino acide identity. You can retrieve all strain data
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) Corynebacterium glutamicum ATCC 14067: N, E.
Corynebacterium glutamicum R: N, E.
Corynebacterium glutamicum ATCC 13032: N, E.
Corynebacterium glutamicum SCgG1: N, E.
Corynebacterium glutamicum Z188: N, E.
Corynebacterium glutamicum S9114: N, E.
Corynebacterium glutamicum K051: N, E.
Corynebacterium glutamicum SCgG2: N, E.
Corynebacterium glutamicum MB001: N, E.
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MSERLNAPQAPIHPITRTHHGIDFVDNYEWLRDKESQETLDYLEAENAFT KQETEQLATLRDNIYEEIKSRVKETDMSIPVRAGKHWYYSRTEEGKSYGY SCRIPVTEGSDAWTPPVIPEGEPAQGETIIMDANELAEGHEFFSMGASSV TTSGRYLAYSTDVTGEERFTLRIKDLETGELLPDTLTGIFYGATWVGEEY LFYQRVDDAWRPDTVWRHKVGTPVEEDVLVYHEPDERYSTWVGTTRSEKF ILFGCASKITSEVRVLPFDQPEGTPEVLIPRAEGVEYDVDHAVVDGSDIW LVTHNAEGPNFSVGWAGVDKLNSLDALAPLVAHKDDVRIEGVDTYRDFII LGYRSGAIGQVAIMKLIDGTFGDFQQLEFDEEIYTVASGGNPEWDAPVIR LSYGSFTTPAQLFNYWIESGERTLLKQQEVLGGYKPSDYVASRLWVTAKD GAQIPVSLVHRTDLDVSKPNPTLLYGYGSYESSIDPGFSIARLSLMDRGM IFAIAHVRGGGEMGRGWYDNGKTTTKKNTFTDFIDVADALIEQKISAPEM LVAEGGSAGGMLMGAIANMAGDRFKAIEANVPFVDPLTSMLMPELPLTVI EWDEWGDPLHDKDVYEYMASYAPYENIEAKNYPNILAVTSLNDTRVLYVE PAKWVAQLRATATGGEFLLKTEMVAGHGGVSGRYEKWRETAFEYGWLINQ ATGVTE
References
Title: Isolation and characterization of a native composite transposon, Tn14751, carrying 17.4 kilobases of Corynebacterium glutamicum chromosomal DNA Inui M, Tsuge Y, Suzuki N, Vertes AA, Yukawa H Ref: Applied Environmental Microbiology, 71:407, 2005 : PubMed
A native composite transposon was isolated from Corynebacterium glutamicum ATCC 14751. This transposon comprises two functional copies of a corynebacterial IS31831-like insertion sequence organized as converging terminal inverted repeats. This novel 20.3-kb element, Tn14751, carries 17.4 kb of C. glutamicum chromosomal DNA containing various genes, including genes involved in purine biosynthesis but not genes related to bacterial warfare, such as genes encoding mediators of antibiotic resistance or extracellular toxins. A derivative of this element carrying a kanamycin resistance cassette, minicomposite Tn14751, transposed into the genome of C. glutamicum at an efficiency of 1.8 x 10(2) transformants per mug of DNA. Random insertion of the Tn14751 derivative carrying the kanamycin resistance cassette into the chromosome was verified by Southern hybridization. This work paves the way for realization of the concept of minimum genome factories in the search for metabolic engineering via genome-scale directed evolution through a combination of random and directed approaches.
The complete genomic sequence of Corynebacterium glutamicum ATCC 13032, well-known in industry for the production of amino acids, e.g. of L-glutamate and L-lysine was determined. The C. glutamicum genome was found to consist of a single circular chromosome comprising 3282708 base pairs. Several DNA regions of unusual composition were identified that were potentially acquired by horizontal gene transfer, e.g. a segment of DNA from C. diphtheriae and a prophage-containing region. After automated and manual annotation, 3002 protein-coding genes have been identified, and to 2489 of these, functions were assigned by homologies to known proteins. These analyses confirm the taxonomic position of C. glutamicum as related to Mycobacteria and show a broad metabolic diversity as expected for a bacterium living in the soil. As an example for biotechnological application the complete genome sequence was used to reconstruct the metabolic flow of carbon into a number of industrially important products derived from the amino acid L-aspartate.
Corynebacterium glutamicum (Brevibacterium flavum), hypothetical protein cgl0343