Gene_locus Report for: crigr-a0a061hy43 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Uncharacterized protein
Relationship
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) cellular organisms: N E > Eukaryota: N E > Opisthokonta: N E > Metazoa: N E > Eumetazoa: N E > Bilateria: N E > Deuterostomia: N E > Chordata: N E > Craniata: N E > Vertebrata: N E > Gnathostomata: N E > Teleostomi: N E > Euteleostomi: N E > Sarcopterygii: N E > Dipnotetrapodomorpha: N E > Tetrapoda: N E > Amniota: N E > Mammalia: N E > Theria: N E > Eutheria: N E > Boreoeutheria: N E > Euarchontoglires: N E > Glires: N E > Rodentia: N E > Myomorpha: N E > Muroidea: N E > Cricetidae: N E > Cricetinae: N E > Cricetulus: N E > Cricetulus griseus: N E ABHD6-Lip :
crigr-g3hfm0 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) Monoacylglycerol lipase ABHD6 .
ABHD8 :
crigr-a0a061ijg4 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Abhydrolase domain-containing protein 8 .
ABHD10 :
crigr-g3h083 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Abhydrolase domain-containing protein 10, mitochondrial .
ABHD11-Acetyl_transferase :
crigr-g3ily8 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Abhydrolase domain-containing protein 11 .
ABHD13-BEM46 :
crigr-g3gtp1 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Abhydrolase domain-containing protein 13 .
ABHD16 :
crigr-g3hh02 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Uncharacterized protein C20orf135-like .
ACHE :
crigr-g3igt0 Cricetulus griseus (Chinese hamster) Acetylcholinesterase .
Arb2_FAM172A :
crigr-a0a061ihy9 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Arb2 domain containing protein .
Arylacetamide_deacetylase :
crigr-a0a061igd5 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Arylacetamide deacetylase-like 4-like protein ,
crigr-g3i9k7 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Arylacetamide deacetylase ,
crigr-g3i9k8 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Arylacetamide deacetylase-like 2 .
BCHE :
crigr-a0a061ikl2 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Cholinesterase .
Carb_B_Chordata :
crigr-g3hzj7 Cricetulus griseus (Chinese hamster) Liver carboxylesterase 31 ,
crigr-g3i7x4 Cricetulus griseus (Chinese hamster) Liver carboxylesterase 22 ,
crigr-g3i7x7 Cricetulus griseus (Chinese hamster) Liver carboxylesterase 1 ,
crigr-g3i766 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) Liver carboxylesterase ,
crigr-g3i767.2 Cricetulus griseus (Chinese hamster) Liver carboxylesterase ,
crigr-g3i769 Cricetulus griseus (Chinese hamster) Liver carboxylesterase ,
crigr-g3iig1 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) Liver carboxylesterase ,
crigr-g3iig3 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) Carboxylesterase 2 ,
crigr-a0a061i741 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Liver carboxylesterase-like protein ,
crigr-a0a061i7x9 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Liver carboxylesterase B-1-like protein ,
crigr-a0a061i8d9 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Carboxylesterase 5A-like protein ,
crigr-a0a061i734 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Carboxylesterase 4A-like protein ,
crigr-a0a061i9z6 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Liver carboxylesterase-like isoform 1 ,
crigr-a0a061iat4 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Liver carboxylesterase-like isoform 1 ,
crigr-a0a061ibf9 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Liver carboxylesterase 1-like protein ,
crigr-a0a061i6l1 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Liver carboxylesterase-like isoform 1 ,
crigr-a0a061idx0 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Liver carboxylesterase 1-like protein ,
crigr-a0a061iej6 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Liver carboxylesterase-like isoform 1 ,
crigr-a0a061iam3 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Carboxylesterase 3 .
Cholesterol_esterase :
crigr-g3iim8 Cricetulus griseus (Chinese hamster) Bile salt-activated lipase .
CIB-CCG1-interacting-factor-B :
crigr-g3h894 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Abhydrolase domain-containing protein 14B .
CMBL :
crigr-g3h7k6 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Carboxymethylenebutenolidase-like .
FSH1 :
crigr-g3hvc7 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Diphthamide biosynthesis protein 1 .
Hepatic_Lipase :
crigr-g3grm1 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Hepatic triacylglycerol lipase .
Kynurenine-formamidase :
crigr-g3h3f6 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). N-formylkynurenine formamidase .
Lipase_3 :
crigr-g3ifk5 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Sn1-specific diacylglycerol lipase alpha .
Lipoprotein_Lipase :
crigr-a0a061ika1 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Lipoprotein lipase ,
crigr-g3hqj0 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Endothelial lipase .
Maspardin-ACP33-SPG21_like :
crigr-g3huz3 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) Maspardin .
Neuroligin :
crigr-g3i7k0 Cricetulus griseus (Chinese hamster) Neuroligin-3 .
NLS3-Tex30 :
crigr-g3h1r9 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) Uncharacterized protein KIAA1310 ,
crigr-g3idw2 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) Uncharacterized protein C13orf27-like .
Pancreatic_lipase :
crigr-g3in33 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Pancreatic lipase .
Phospholipase :
crigr-g3i1j5 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Phospholipase A1 member A ,
crigr-g3hkm8 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Lipase member H .
SERHL :
crigr-a0a3l7ib08 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). SERHL .
Valacyclovir-hydrolase :
crigr-g3ieg5 Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) Valacyclovir hydrolase Molecular evidence
Database
No mutation No structure No kinetic No Substrate No inhibitor
Sequence
Graphical view for this peptide sequence: crigr-a0a061hy43 Colored MSA for Pectinacetylesterase-Notum (raw)
MTLSVPAYYLKESKGSRRWLLFLEGGWYCFNRENCDSRYSTMRRLMSSKD
WPHTRTGTGILSSQPEENPHWWNANMVFIPYCSSDVWSGASPKPEKNEYA
FMGSLIIQEVVRELLGQGLSGAKVLLLAGSSAGGTGVLLNVDRVAELLEE
LGYPSIQVRGLADSGWFLDNKQYRRSDCIDTINCAPTEAIRRGIRYWNGM
VPERCQRQFKEGEEWNCFFGYKVYPTLRCPVFVVQWLFDEAQLTVDNVHL
TGQPVQEGQWLYIQNLGRELRGTLKDVQASFAPACLSHEIIIRSYWTDVQ
VKGTSLPRALHCWDRSFHDSHKASKTPMKGCPFHLVDSCPWPHCNPSCPT
IRDQFTGQEMNVAQFLMHLGFDVQTVAQQQGMEPSKLLGMLSGGS
Legend
This sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA
M T L S V P A Y Y L K E S K G S R R W L L F L E G G W Y C F N R E N C D S R Y S T M R R L M S S K D W P H T R T G T G I L S S Q P E E N P H W W N A N M V F I P Y C S S D V W S G A S P K P E K N E Y A F M G S L I I Q E V V R E L L G Q G L S G A K V L L L A G S S A G G T G V L L N V D R V A E L L E E L G Y P S I Q V R G L A D S G W F L D N K Q Y R R S D C I D T I N C A P T E A I R R G I R Y W N G M V P E R C Q R Q F K E G E E W N C F F G Y K V Y P T L R C P V F V V Q W L F D E A Q L T V D N V H L T G Q P V Q E G Q W L Y I Q N L G R E L R G T L K D V Q A S F A P A C L S H E I I I R S Y W T D V Q V K G T S L P R A L H C W D R S F H D S H K A S K T P M K G C P F H L V D S C P W P H C N P S C P T I R D Q F T G Q E M N V A Q F L M H L G F D V Q T V A Q Q Q G M E P S K L L G M L S G G S
Reference
Title: The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell lineXu X , Nagarajan H , Lewis NE , Pan S , Cai Z , Liu X , Chen W , Xie M , Wang W and Palsson BO <12 more author(s)> Xu X , Nagarajan H , Lewis NE , Pan S , Cai Z , Liu X , Chen W , Xie M , Wang W , Hammond S , Andersen MR , Neff N , Passarelli B , Koh W , Fan HC , Wang J , Gui Y , Lee KH , Betenbaugh MJ , Quake SR , Famili I , Palsson BO (- 12) Ref: Nat Biotechnol, 29 :735, 2011 : PubMed Abstract ESTHER: Xu_2011_Nat.Biotechnol_29_735 PubMedSearch: Xu 2011 Nat.Biotechnol 29 735 PubMedID: 21804562 Gene_locus related to this paper: crigr-g3hfm0 ,
crigr-g3h894 ,
crigr-a0a061hy43 ,
crigr-g3h3f6 ,
crigr-g3i9k7 ,
crigr-g3i9k8 ,
crigr-g3grm1 ,
crigr-g3in33 ,
crigr-g3i1j5 ,
crigr-a0a061ika1 ,
crigr-g3hqj0 ,
crigr-g3hh02 ,
crigr-g3h083 ,
crigr-a0a3l7ib08 ,
crigr-a0a061ihy9 ,
crigr-g3ifk5 ,
crigr-g3ily8 ,
crigr-g3hvc7 ,
crigr-g3gtp1 ,
crigr-g3h7k6 ,
crigr-g3hkm8 Abstract
Chinese hamster ovary (CHO)-derived cell lines are the preferred host cells for the production of therapeutic proteins. Here we present a draft genomic sequence of the CHO-K1 ancestral cell line. The assembly comprises 2.45 Gb of genomic sequence, with 24,383 predicted genes. We associate most of the assembled scaffolds with 21 chromosomes isolated by microfluidics to identify chromosomal locations of genes. Furthermore, we investigate genes involved in glycosylation, which affect therapeutic protein quality, and viral susceptibility genes, which are relevant to cell engineering and regulatory concerns. Homologs of most human glycosylation-associated genes are present in the CHO-K1 genome, although 141 of these homologs are not expressed under exponential growth conditions. Many important viral entry genes are also present in the genome but not expressed, which may explain the unusual viral resistance property of CHO cell lines. We discuss how the availability of this genome sequence may facilitate genome-scale science for the optimization of biopharmaceutical protein production.
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