Gene_locus Report for: drome-1vite

The fly Drosophila melanogaster is one of the most intensively studied organisms in biology and serves as a model system for the investigation of many developmental and cellular processes common to higher eukaryotes, including humans. We have determined the nucleotide sequence of nearly all of the approximately 120-megabase euchromatic portion of the Drosophila genome using a whole-genome shotgun sequencing strategy supported by extensive clone-based sequence and a high-quality bacterial artificial chromosome physical map. Efforts are under way to close the remaining gaps; however, the sequence is of sufficient accuracy and contiguity to be declared substantially complete and to support an initial analysis of genome structure and preliminary gene annotation and interpretation. The genome encodes approximately 13,600 genes, somewhat fewer than the smaller Caenorhabditis elegans genome, but with comparable functional diversity.

We have isolated recombinant DNA clones coding for female specific proteins from Drosophila melanogaster. By screening with 32P-(A)+RNA from male and female flies, respectively, we were able to isolate a set of 100 cDNA clones which showed a positive hybridization signal for RNA from female flies. These clones have been rescreened with RNA isolated from fat body of two day old male and female flies. We obtained four positive cDNA clones. Isolation of the corresponding genomic sequences, construction of the physical map and comparing it with the restriction maps published by Barnett et al. (1) led us to conclude that we had isolated the genes coding for two of the three known yolk protein precursors (vitellogenins), YP I and YP II. The sequence of the YP I gene was determined. It gives rise to a protein of 48 700 dalton MW which might be cleaved to a MW of 46 700 during transport. The coding sequence is interrupted by a single intron of 75 bases in length. The proposed leader sequence starts at a region homologous to six heat shock gene sequences at the site of initiation of transcription, suggesting the existence of an 11 bp cap specific consensus sequence for Drosophila melanogaster.

We have determined the complete nucleotide sequence of the hormonally regulated yolk protein 1 (YP1) gene of Drosophila melanogaster. We have also determined the sequence location of the 5' and 3' ends of both the mature mRNA and the gene's only intron. The YP1 gene contains sequences similar to those found in many other eukaryotic genes. Among these sequences are the Hogness-Goldberg box, the capping site, the ribosome binding site and the polyadenylation signal sequence, all perhaps involved in transcriptional or translational control. Also among these sequences are the consensus splice sequences. They occur at each end of the 76 nucleotide intron. One distinctive secondary structure likely to occur in either the DNA or RNA and which might be involved in the regulation of transcription or translation was also found in the YP1 gene sequence. We show the protein sequence predicted by the DNA sequence and the RNA mapping results.

The fly Drosophila melanogaster is one of the most intensively studied organisms in biology and serves as a model system for the investigation of many developmental and cellular processes common to higher eukaryotes, including humans. We have determined the nucleotide sequence of nearly all of the approximately 120-megabase euchromatic portion of the Drosophila genome using a whole-genome shotgun sequencing strategy supported by extensive clone-based sequence and a high-quality bacterial artificial chromosome physical map. Efforts are under way to close the remaining gaps; however, the sequence is of sufficient accuracy and contiguity to be declared substantially complete and to support an initial analysis of genome structure and preliminary gene annotation and interpretation. The genome encodes approximately 13,600 genes, somewhat fewer than the smaller Caenorhabditis elegans genome, but with comparable functional diversity.

Clones of genes coding for two of the yolk protein precursors from Drosophila melanogaster, YPI and YPII have been isolated. A single small intron was located in the YPII coding region at about the same position as it is found in YPI (6). The entire intergenic spacer region and most of the exon I from the YPII gene have been sequenced. The "capping" sites for both mRNAs have been determined using the S1 protection and cDNA synthesis methods. Comparison of the sequences which might be involved in transcriptional or translational control of these genes reveals for YPII a Hogness Goldberg box but no indication for a conserved sequence around-70-80 (CAT box). In contrast to YPI it resembles no significant homology to the 3' end of 18S rRNA. The first 60 amino acids of exon I from both genes share little homology except for the hydrophobic amino acids which should be involved in protein secretion.

We have isolated recombinant DNA clones coding for female specific proteins from Drosophila melanogaster. By screening with 32P-(A)+RNA from male and female flies, respectively, we were able to isolate a set of 100 cDNA clones which showed a positive hybridization signal for RNA from female flies. These clones have been rescreened with RNA isolated from fat body of two day old male and female flies. We obtained four positive cDNA clones. Isolation of the corresponding genomic sequences, construction of the physical map and comparing it with the restriction maps published by Barnett et al. (1) led us to conclude that we had isolated the genes coding for two of the three known yolk protein precursors (vitellogenins), YP I and YP II. The sequence of the YP I gene was determined. It gives rise to a protein of 48 700 dalton MW which might be cleaved to a MW of 46 700 during transport. The coding sequence is interrupted by a single intron of 75 bases in length. The proposed leader sequence starts at a region homologous to six heat shock gene sequences at the site of initiation of transcription, suggesting the existence of an 11 bp cap specific consensus sequence for Drosophila melanogaster.

We have determined the complete nucleotide sequence of the hormonally regulated yolk protein 1 (YP1) gene of Drosophila melanogaster. We have also determined the sequence location of the 5' and 3' ends of both the mature mRNA and the gene's only intron. The YP1 gene contains sequences similar to those found in many other eukaryotic genes. Among these sequences are the Hogness-Goldberg box, the capping site, the ribosome binding site and the polyadenylation signal sequence, all perhaps involved in transcriptional or translational control. Also among these sequences are the consensus splice sequences. They occur at each end of the 76 nucleotide intron. One distinctive secondary structure likely to occur in either the DNA or RNA and which might be involved in the regulation of transcription or translation was also found in the YP1 gene sequence. We show the protein sequence predicted by the DNA sequence and the RNA mapping results.