(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Opisthokonta: NE > Metazoa: NE > Eumetazoa: NE > Bilateria: NE > Protostomia: NE > Ecdysozoa: NE > Panarthropoda: NE > Arthropoda: NE > Mandibulata: NE > Pancrustacea: NE > Hexapoda: NE > Insecta: NE > Dicondylia: NE > Pterygota: NE > Neoptera: NE > Holometabola: NE > Diptera: NE > Brachycera: NE > Muscomorpha: NE > Eremoneura: NE > Cyclorrhapha: NE > Schizophora: NE > Acalyptratae: NE > Ephydroidea: NE > Drosophilidae: NE > Drosophilinae: NE > Drosophilini: NE > Drosophila [fruit fly, genus]: NE > Drosophila [fruit fly, subgenus]: NE > virilis group: NE > Drosophila virilis: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MTQILLPIALLCLFAASTLSNPLLVELPNGELRGRDNGFYYSYESIPYAE PPIDDLCLEEPRPYTERWENTFDATRPPVECLQWSQLISQPNKLTGSEDC LTVSIYKPKNLTRISFPVVAHIFGGGWSFGGAIDDGVEPFMNSGNVIVVK INHRVGALGFMSTGDSVIPGNFGLKDQRLAIKWIRNNIARFGGDPHNIIL LGFGTGGSSVHLQLMHKDMEQLVKGAISISGTATVPWAVQANARDLAFRY GKLLGCNNPKNSRELKDCLKKTDAEEFVSTLRHLQVFDYVPFGPFGPVVE SPEVESPFLTELPLDTIRSGNFAQVPWLASYTPENGIYNAALLLAKDANG KERIEELNTRWNELAPYFLAYPYTLKRSEMNAHSQKLKYQYLGYKNFSVE NYFDVQRLFTNELYKKGIELSLDAHRKHGASPVYAYVYDNPADKSLAQFL AKRSDISLGTGMGDDYYLLMNNPLREPLRADEKIVSWKLVKMVEDFAAHE TLVYDDCVFPNNLGKKKFQLVVIGRNYCKQLEVDRLPDTVSNNLPPNPIV IY
MOTIVATION: Many genomes are sequenced by a collaboration of several centers, and then each center produces an assembly using their own assembly software. The collaborators then pick the draft assembly that they judge to be the best and the information contained in the other assemblies is usually not used. METHODS: We have developed a technique that we call assembly reconciliation that can merge draft genome assemblies. It takes one draft assembly, detects apparent errors, and, when possible, patches the problem areas using pieces from alternative draft assemblies. It also closes gaps in places where one of the alternative assemblies has spanned the gap correctly. RESULTS: Using the Assembly Reconciliation technique, we produced reconciled assemblies of six Drosophila species in collaboration with Agencourt Bioscience and The J. Craig Venter Institute. These assemblies are now the official (CAF1) assemblies used for analysis. We also produced a reconciled assembly of Rhesus Macaque genome, and this assembly is available from our website http://www.genome.umd.edu. AVAILABILITY: The reconciliation software is available for download from http://www.genome.umd.edu/software.htm
The esterase S gene (estS) of Drosophila virilis is specifically expressed in the ejaculatory bulbs of males. Its sequencing shows similarities between estS product and other esterases of different origin. The transcription of estS in ejaculatory bulbs is at least 2 orders of magnitude higher than in other tissues of males. Two promoters, P1 (distal) and P2 (proximal), and two different transcripts were identified. The promoter P2 is used much more efficiently, and in a stringent, tissue-specific manner. The transcription from P1 takes place in different tissues and stages of development of D. virilis. However, the mRNA transcribed from P1 seems to be inactive in translation as there are three open-reading frames (ORF) between P1 and P2, which may block the translation in P1 initiated mRNA. Insertion of sequence containing the three ORFs into the 5' untranslated region of the CAT gene strongly inhibited expression of CAT. Point mutations destroying the three ORFs completely eliminate the inhibitory effect. Hence tissue-specific expression of the estS gene may depend on control at the level of transcription, promoter selection and translation.
Title: [Primary structure of the transcription initiation region of the esterase S gene of Drosophila virilis] Enikolopov GN, Khechumian RK, Kastil'o K, Georgiev GP Ref: Bioorganicheskaia Khimiia, 14:1671, 1988 : PubMed
The transcription initiation region of the gene for tissue-specific esterase S of D. virilis has been analysed. By means of high-resolution S1-mapping we located a set of transcription initiation points, two of them being major ones. The position of the major start points, but not the efficiency of their usage, does not depend on age, sex or strain of the flies. The transcription initiation region is sequenced. The region contains various motifs characteristic for eukaryotic promoters.
