(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Viridiplantae: NE > Streptophyta: NE > Streptophytina: NE > Embryophyta: NE > Tracheophyta: NE > Euphyllophyta: NE > Spermatophyta: NE > Magnoliophyta: NE > Mesangiospermae: NE > eudicotyledons: NE > Gunneridae: NE > Pentapetalae: NE > rosids: NE > malvids: NE > Malvales: NE > Malvaceae: NE > Helicteroideae: NE > Durio: NE > Durio zibethinus: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MDSHTLGEITGFREELIKKACSIAMAAHKSQTKPYIAEKSRTSSDVLFSF PGSWSVGHWFTRQPFGEIKVDQSELLSPMGNDKVATSLRSIGCDELATVN EGFLRRLEMILSNSSLQKEVEKANTEQKQIVFTGHSSGGAIAILATVWFL EQYFKPKKTGMSPLCVTFGSPFVADFIFNHALRRENWSNYFLHFVMRYDI VPRVLFAPLSSMQRELEQILYLLNQKARNSIQGSIAEASKFYQTVMRNAS ALASHAACQLMGNANPILETVASFINLSPYRPSGTFVFCTGNGKLVVVRN ADAILQLLFYSSQLCSENELEAIAERSLNNHFGYRSELQESLSKQNVVNL DHLEGLPLSSNGAAAENIAINMALTDLGLSTRARLCLRAAGEQEKQKLSN QQIMDKKKKDIDKGLAVLEEYKNKSAVCQVGYYDAFKISKDVDDFQANVK RLELTGIWDEIIEMLNRHELPDGFESEKDWVELATKYRRIVEPLDIANYY RHAKNEDTGPYMHKGRPRRYRYTQRWLEHALRMPVGSSEESCFWAEVEEL LLLYQGKDFKPGAFEDIRERILNLERKLEEWIHGNQISNDVFLEGSTFTK WWVSLPHQHKYVSRIRGLMNR
Durian (Durio zibethinus) is a Southeast Asian tropical plant known for its hefty, spine-covered fruit and sulfury and onion-like odor. Here we present a draft genome assembly of D. zibethinus, representing the third plant genus in the Malvales order and first in the Helicteroideae subfamily to be sequenced. Single-molecule sequencing and chromosome contact maps enabled assembly of the highly heterozygous durian genome at chromosome-scale resolution. Transcriptomic analysis showed upregulation of sulfur-, ethylene-, and lipid-related pathways in durian fruits. We observed paleopolyploidization events shared by durian and cotton and durian-specific gene expansions in MGL (methionine gamma-lyase), associated with production of volatile sulfur compounds (VSCs). MGL and the ethylene-related gene ACS (aminocyclopropane-1-carboxylic acid synthase) were upregulated in fruits concomitantly with their downstream metabolites (VSCs and ethylene), suggesting a potential association between ethylene biosynthesis and methionine regeneration via the Yang cycle. The durian genome provides a resource for tropical fruit biology and agronomy.
