LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MHQIFPSTFFNFEFLRLLGTAPYLGAETGECLATAARIKDGDPESWYQAW YEQAQKALALADEAKAVGDGPGAAWGYIRASNYFRASEFLLHCTPEDPRI LSSAVASADAFDKGWILLDGSVRKVEIPYEGGNTLPGRLYLPAPHHQVSG KIPVVVQTGGFDSTQEELYYYGAAGALPRGYAVFSFDGPGQGLSLRKDKL YLRPDWEHVTSKVLDHVIGELAPVHNLDVDRLAVFGASLGGYLSLRAAAD PRVKAVVSCDGPLDLFDITRSRMPPWFINGWLSGWLSDGFFNWVIDRLAS VNFQLAWEFGHSKWVYGVKTPADVMRTMQKFSLKDGYLSKIKCPTLITGA ADSFYFTPQQNAHPIFDSLSALGPAEKHLWIGKDVEGGGLQAKIGALALM HHKMFAWLDETFGIRRDEL
We identified the biosynthetic gene cluster for lucilactaene, a cell cycle inhibitor from a filamentous fungus Fusarium sp. RK 97-94. The luc1 knockout strain accumulated demethylated analogs, indicating the involvement of Luc1 methyltransferase in lucilactaene biosynthesis. Lucilactaene showed potent antimalarial activity. Our data suggested that methylation and ether ring formation are essential for its potent antimalarial activity.
