human-ABHD6

 
Homo sapiens (Human) ABHD6 Monoacylglycerol lipase EC: 3.1.1.23

Comment
Monoacylglycerol lipase ABHD6 hydrolyses the endocannabinoid 2-arachidonoylglycerol (2-AG). 2-AG regulates neurotransmission and neuroinflammation by activating CB1 cannabinoid receptors on neurons and CB2 cannabinoid receptors on microglia. (A MGLL human-MGLL or MAGLL is the other enzympe hydrolyzing 26AG.(belong to Monoglyceridelipase_lysophospholip family) ABHD6 of animals is homologous to some bacterial enzymes. For bacterial enzymes, this family correspond to family V.1 of the classification of Arpigny and Jaeger 1999. Alpha/beta-Hydrolase domain 6 deletion induces adipose browning and prevents obesity and type 2 diabetes and is published by Zhao et al. A study by Wei et al. shows that ABHD6 negatively regulates the surface delivery and synaptic function of AMPA receptors independantly of hydrolase activity Q9BV23 and Q9HBL9 differ only at cterminus (alternative splicing?)Q6ZMF7 hypothetical protein flj23958


Relationship
Family ABHD6-Lip
Block X
Homo sapiens position in NCBI Life Tree :
N link to NCBI taxonomic web page and E link to ESTHER gene locus found in this strain.
> cellular organisms: N E > Eukaryota: N E > Opisthokonta: N E > Metazoa: N E > Eumetazoa: N E > Bilateria: N E > Deuterostomia: N E > Chordata: N E > Craniata: N E > Vertebrata: N E > Gnathostomata: N E > Teleostomi: N E > Euteleostomi: N E > Sarcopterygii: N E > Dipnotetrapodomorpha: N E > Tetrapoda: N E > Amniota: N E > Mammalia: N E > Theria: N E > Eutheria: N E > Boreoeutheria: N E > Euarchontoglires: N E > Primates: N E > Haplorrhini: N E > Simiiformes: N E > Catarrhini: N E > Hominoidea: N E > Hominidae: N E > Homininae: N E > Homo: N E > Homo sapiens: N E

Molecular evidence
Database
No mutation
1 structure:
7OTS: Crystal structure of human Monoacylglycerol Lipase ABHD6 in complex with oleic acid and octyl glucoside
No kinetic

Substrate: PGE2-glyceryl-ester , Bis(monoacylglycerol)phosphate , AHMMCE , Octylglucoside , 2-Arachidonylglycerol ,
Inhibitor: HT-01 , JCP-265 , KT203 , KT182 , LEI-106 , BIA-10-2474 , Palmostatin-B , JZL195 , WWL113 , AM6701 , WWL70 , LY2183240 ,
>3 Genbank links 2 more: BC001698, AF225418, AK172797
>3 UniProt links 1 more: Q9BV23, C9JNE7, C9J010
1 Structure : 7OTS
>3 UniProtTrembl links 1 more: Q9BV23, Q9HBL9, C9JNE7
>3 Interpro links 1 more: Q9BV23, Q9HBL9, C9JNE7
>3 Prodom links 1 more: Q9BV23, Q9HBL9, C9JNE7
>3 Pfam links 1 more: Q9BV23, Q9HBL9, C9JNE7
>3 PIRSF links 1 more: Q9BV23, Q9HBL9, C9JNE7
>3 SUPERFAM links 1 more: Q9BV23, Q9HBL9, C9JNE7
>3 QuickSwissBlast links 1 more: Q9BV23, Q9HBL9, C9JNE7
1 EntrezGene : 57406
1 SNP : 57406
1 UniGene : 476454
1 HUGO HGNC : 21398
1 Ensembl : ENSG00000163686
 
Sequence
Graphical view for this peptide sequence: human-ABHD6
Colored MSA for ABHD6-Lip (raw)
MDLDVVNMFVIAGGTLAIPILAFVASFLLWPSALIRIYYWYWRRTLGMQV
RYVHHEDYQFCYSFRGRPGHKPSILMLHGFSAHKDMWLSVVKFLPKNLHL
VCVDMPGHEGTTRSSLDDLSIDGQVKRIHQFVECLKLNKKPFHLVGTSMG
GQVAGVYAAYYPSDVSSLCLVCPAGLQYSTDNQFVQRLKELQGSAAVEKI
PLIPSTPEEMSEMLQLCSYVRFKVPQQILQGLVDVRIPHNNFYRKLFLEI
VSEKSRYSLHQNMDKIKVPTQIIWGKQDQVLDVSGADMLAKSIANCQVEL
LENCGHSVVMERPRKTAKLIIDFLASVHNTDNNKKLD
Legend This sequence has been compared to family alignement (MSA)
red => minority aminoacid
blue => majority aminoacid
color intensity => conservation rate
title => sequence position(MSA position)aminoacid rate
Catalytic site
Catalytic site in the MSA

MDLDVVNMFVIAGGTLAIPILAFVASFLLWPSALIRIYYWYWRRTLGMQV
RYVHHEDYQFCYSFRGRPGHKPSILMLHGFSAHKDMWLSVVKFLPKNLHL
VCVDMPGHEGTTRSSLDDLSIDGQVKRIHQFVECLKLNKKPFHLVGTSMG
GQVAGVYAAYYPSDVSSLCLVCPAGLQYSTDNQFVQRLKELQGSAAVEKI
PLIPSTPEEMSEMLQLCSYVRFKVPQQILQGLVDVRIPHNNFYRKLFLEI
VSEKSRYSLHQNMDKIKVPTQIIWGKQDQVLDVSGADMLAKSIANCQVEL
LENCGHSVVMERPRKTAKLIIDFLASVHNTDNNKKLD

no DNA




References
39 more
    Title: ABHD6 Controls Amphetamine-Stimulated Hyperlocomotion: Involvement of CB(1) Receptors
    Deng L, Viray K, Singh S, Cravatt B, Stella N
    Ref: Cannabis Cannabinoid Res, :, 2021 : PubMed

            

    Title: Carnitine palmitoyltransferase 1C negatively regulates the endocannabinoid hydrolase ABHD6 in mice, depending on nutritional status
    Miralpeix C, Reguera AC, Fosch A, Casas M, Lillo J, Navarro G, Franco R, Casas J, Alexander SPH and Rodriguez-Rodriguez R <1 more author(s)>
    Ref: British Journal of Pharmacology, 178:1507, 2021 : PubMed

            

    Title: Termination of acute stress response by the endocannabinoid system is regulated through LSD1-mediated transcriptional repression of 2-AG hydrolases ABHD6 and MAGL
    Longaretti A, Forastieri C, Gabaglio M, Rubino T, Battaglioli E, Rusconi F
    Ref: Journal of Neurochemistry, :e15000, 2020 : PubMed

            


Other Papers


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Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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