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Gene_locus Report for: humla-1lipa

Thermomyces lanuginosus (Humicola lanuginosa) lipase (lip); Lipozyme TL; LIP_THELA lipolase TLL

Comment
Novozymes A/S Lipozym TL is a 1,3 specific lipase originating from Thermomyces lanuginosus. Lipozym TL IM is an imobilized form of the enzyme on a non-compressible silica gel carrier. Lipozyme TL IM is a highly effective catalyst for interesterification and can rearrange fatty acids preferentially, but not uniquely in the 1-and 3 positions of the triglycerides. Lipozyme TL IM exhibits a high degree of substrate selectivity allowing bulky side chains/large groups on the alcohol and acid part of the molecule. Lipozyme TL Lipolase TLL


Relationship
Family|Lipase_3
Block| L
Position in NCBI Life Tree|Thermomyces lanuginosus
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms: N E > Eukaryota: N E > Opisthokonta: N E > Fungi: N E > Dikarya: N E > Ascomycota: N E > saccharomyceta: N E > Pezizomycotina: N E > leotiomyceta: N E > Eurotiomycetes: N E > Eurotiomycetidae: N E > Eurotiales: N E > Trichocomaceae: N E > Thermomyces: N E > Thermomyces lanuginosus: N E


Molecular evidence
Database
No mutation
29 structures (e.g. : 1DT3, 1DT5, 1DTE... more)
No kinetic





6 substrates (e.g. : 2-hydroxy-3-(octadecanoyloxy)propyl-pentacosanoate, CNDE, Ethyl-acetoacetate... more)
3 inhibitors: Decanoate, Laurate, Palmitate
1 Genbank : AF054513
1 UniProt : O59952
1 Ncbi-nid : 2997732
1 Ncbi-pid : 2997733
>3 Structure links 26 more: 1TIB, 1DT3, 1DT5
1 UniProt : O59952
1 Interpro : O59952
1 Prodom : O59952
1 Pfam : O59952
1 PIRSF : O59952
1 SUPERFAM : O59952
Sequence
Graphical view for this peptide sequence: humla-1lipa
Colored MSA for Lipase_3 (raw)
MRSSLVLFFVSAWTALASPIRREVSQDLFNQFNLFAQYSAAAYCGKNNDA
PAGTNITCTGNACPEVEKADATFLYSFEDSGVGDVTGFLALDNTNKLIVL
SFRGSRSIENWIGNLNFDLKEINDICSGCRGHDGFTSSWRSVADTLRQKV
EDAVREHPDYRVVFTGHSLGGALATVAGADLRGNGYDIDVFSYGAPRVGN
RAFAEFLTVQTGGTLYRITHTNDIVPRLPPREFGYSHSSPEYWIKSGTLV
PVTRNDIVKIEGIDATGGNNQPNIPDIPAHLWYFGLIGTCL
Legend This sequence has been compared to family alignement (MSA)
red => minority aminoacid
blue => majority aminoacid
color intensity => conservation rate
title => sequence position(MSA position)aminoacid rate
Catalytic site
Catalytic site in the MSA

MRSSLVLFFVSAWTALASPIRREVSQDLFNQFNLFAQYSAAAYCGKNNDA
PAGTNITCTGNACPEVEKADATFLYSFEDSGVGDVTGFLALDNTNKLIVL
SFRGSRSIENWIGNLNFDLKEINDICSGCRGHDGFTSSWRSVADTLRQKV
EDAVREHPDYRVVFTGHSLGGALATVAGADLRGNGYDIDVFSYGAPRVGN
RAFAEFLTVQTGGTLYRITHTNDIVPRLPPREFGYSHSSPEYWIKSGTLV
PVTRNDIVKIEGIDATGGNNQPNIPDIPAHLWYFGLIGTCL


References
42 more
    Title: The Crystal Structures of Thermomyces (Humicola) Lanuginosa Lipase in Complex with Enzymatic Reactants
    McPherson A, Larson SB, Kalasky A
    Ref: Curr Enzym Inhib, 16:, 2020 : PubMed

            

    Title: A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography
    de Wijn R, Hennig O, Roche J, Engilberge S, Rollet K, Fernandez-Millan P, Brillet K, Betat H, Morl M and Sauter C <7 more author(s)>
    Ref: IUCrJ, 6:454, 2019 : PubMed

            

    Title: Lipases That Activate at High Solvent Polarities
    Skjold-Jorgensen J, Vind J, Svendsen A, Bjerrum MJ
    Ref: Biochemistry, 55:146, 2016 : PubMed

            


Other Papers


Send your questions or comments to :
Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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