(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Viridiplantae: NE > Streptophyta: NE > Streptophytina: NE > Embryophyta: NE > Tracheophyta: NE > Euphyllophyta: NE > Spermatophyta: NE > Magnoliophyta: NE > Mesangiospermae: NE > eudicotyledons: NE > Gunneridae: NE > Pentapetalae: NE > rosids: NE > fabids: NE > Malpighiales: NE > Euphorbiaceae: NE > Crotonoideae: NE > Jatropheae: NE > Jatropha: NE > Jatropha curcas: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MSIVAEAPGYLQVFSDGSVKRFAPETFPSRQPHNKYKFKDLVIDPSKPIT ARLFLPESSASRLPVLVYFHGGGFCVGSTTWLGYHHFLADFSVTSQSIVL SIDYRLAPENRLPIAYDDCYSSLEWLSHQVSVEPWLQSADLSRVFLSGDS AGGNISHNVAIRASSDKLPHVKIKGLLLIHPYFGSQERTEKEMADGAAKD VEMNDMFWRLSIPEGSNRDYYGCNFEIQDLSVAKWKEFPAAVVYVAGLDF LKERGVMYTLFLAKQGVKEVELVEAEGESHIFHVFSPDSEATRLLQQQMS KFIQSH
Reference
Title: Global analysis of gene expression profiles in physic nut (Jatropha curcas L.) seedlings exposed to salt stress Zhang L, Zhang C, Wu P, Chen Y, Li M, Jiang H, Wu G Ref: PLoS ONE, 9:e97878, 2014 : PubMed
BACKGROUND: Salt stress interferes with plant growth and production. Plants have evolved a series of molecular and morphological adaptations to cope with this abiotic stress, and overexpression of salt response genes reportedly enhances the productivity of various crops. However, little is known about the salt responsive genes in the energy plant physic nut (Jatropha curcas L.). Thus, excavate salt responsive genes in this plant are informative in uncovering the molecular mechanisms for the salt response in physic nut. METHODOLOGY/PRINCIPAL FINDINGS: We applied next-generation Illumina sequencing technology to analyze global gene expression profiles of physic nut plants (roots and leaves) 2 hours, 2 days and 7 days after the onset of salt stress. A total of 1,504 and 1,115 genes were significantly up and down-regulated in roots and leaves, respectively, under salt stress condition. Gene ontology (GO) analysis of physiological process revealed that, in the physic nut, many "biological processes" were affected by salt stress, particular those categories belong to "metabolic process", such as "primary metabolism process", "cellular metabolism process" and "macromolecule metabolism process". The gene expression profiles indicated that the associated genes were responsible for ABA and ethylene signaling, osmotic regulation, the reactive oxygen species scavenging system and the cell structure in physic nut. CONCLUSIONS/SIGNIFICANCE: The major regulated genes detected in this transcriptomic data were related to trehalose synthesis and cell wall structure modification in roots, while related to raffinose synthesis and reactive oxygen scavenger in leaves. The current study shows a comprehensive gene expression profile of physic nut under salt stress. The differential expression genes detected in this study allows the underling the salt responsive mechanism in physic nut with the aim of improving its salt resistance in the future.
