(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Bacteria: NE > Proteobacteria: NE > Gammaproteobacteria: NE > Legionellales: NE > Legionellaceae: NE > Legionella: NE > Legionella pneumophila: NE
Warning: This entry is a compilation of different species or line or strain with more than 90% amino acid identity. You can retrieve all strain data
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) Legionella pneumophila subsp. pneumophila: N, E.
Legionella pneumophila subsp. pneumophila str. Philadelphia 1: N, E.
Legionella pneumophila str. Lens: N, E.
Legionella pneumophila str. Paris: N, E.
Legionella pneumophila str. Corby: N, E.
Legionella pneumophila 2300/99 Alcoy: N, E.
Legionella pneumophila 130b: N, E.
Legionella pneumophila serogroup 1: N, E.
Legionella pneumophila str. Leg01/11: N, E.
Legionella pneumophila str. Leg01/53: N, E.
Legionella pneumophila str. Leg01/20: N, E.
Legionella pneumophila subsp. pneumophila ATCC 43290: N, E.
Legionella pneumophila subsp. pneumophila str. Thunder Bay: N, E.
Legionella pneumophila subsp. pneumophila LPE509: N, E.
Legionella pneumophila str. 121004: N, E.
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MDKQDIDLPYQMGHDFISYQLDYWQRSILFWDTLRERANNMMEHEQQGLP PLLNFKYELVLDGKSLEPKTNYALVKILEVGDVCFEECFDPHAHPVIIVD PRSGHGPGIGGFKRDSEVGIALHSGHPVYFVIFYPNPVPHQTLADVLMTL RHFVEKVQAWHEGKAPILYGNCQAGWMLALLASDCVGSVGLTVMNGSPVS YWSNSEEEANPMQLLGGLLGGSWSARFLSDLKEGILDGAWLVSNFELLNP TTAIWDKYYGLFDEIDAERERFLEFERWWNGFYQFSQEEIMATVNNLFIG NQLERGEMRIHKGCVYDLKRIQSPIVLFASQGDQITPPRQALHWIRTIYP TTQALKEAKQRVVYLLHPSVGHLGIFVSAKVVRLHHRAILEHGAAIEQLQ PGLYEMIIVNPTGNPDCSKEQYHVRFEARELTELCTTSSTQPFDKVRQTS EANDSIYQKVIQPFVQSLSNPFLSWWLEKTHPMRLSRYVFSEKVNPLMKA IEQVSPPIQANRQRVTESNFFKKTEHAWAQMMRDSLESVRIMRNEVMTNW FDALYKDPD
Legionella pneumophila, the causative agent of Legionnaires' disease, replicates as an intracellular parasite of amoebae and persists in the environment as a free-living microbe. Here we have analyzed the complete genome sequences of L. pneumophila Paris (3,503,610 bp, 3,077 genes), an endemic strain that is predominant in France, and Lens (3,345,687 bp, 2,932 genes), an epidemic strain responsible for a major outbreak of disease in France. The L. pneumophila genomes show marked plasticity, with three different plasmids and with about 13% of the sequence differing between the two strains. Only strain Paris contains a type V secretion system, and its Lvh type IV secretion system is encoded by a 36-kb region that is either carried on a multicopy plasmid or integrated into the chromosome. Genetic mobility may enhance the versatility of L. pneumophila. Numerous genes encode eukaryotic-like proteins or motifs that are predicted to modulate host cell functions to the pathogen's advantage. The genome thus reflects the history and lifestyle of L. pneumophila, a human pathogen of macrophages that coevolved with fresh-water amoebae.
We present the genomic sequence of Legionella pneumophila, the bacterial agent of Legionnaires' disease, a potentially fatal pneumonia acquired from aerosolized contaminated fresh water. The genome includes a 45-kilobase pair element that can exist in chromosomal and episomal forms, selective expansions of important gene families, genes for unexpected metabolic pathways, and previously unknown candidate virulence determinants. We highlight the genes that may account for Legionella's ability to survive in protozoa, mammalian macrophages, and inhospitable environmental niches and that may define new therapeutic targets.
Title: Macrophage-induced genes of Legionella pneumophila: protection from reactive intermediates and solute imbalance during intracellular growth Rankin S, Li Z, Isberg RR Ref: Infect Immun, 70:3637, 2002 : PubMed
A promoter-probe strategy was devised to identify genes specifically expressed by Legionella pneumophila during growth within the macrophage. Random fragments from the L. pneumophila chromosome were inserted upstream of a promoterless phage T4 td gene, and fragments that led to complementation of thymine auxotrophy during intracellular growth of the bacterium were identified. Two different selection strategies were employed to eliminate promoters that were also active during extracellular growth of the bacterium. Some of these genes were identified independently by using both of the selection strategies. The factors identified include orthologs of efflux-mediated resistance determinants and transporters, a transporter involved in protection from osmotic stress, a stress response GTP-binding protein, a response regulator, a sensor kinase, and two systems that increase the reducing potential of the bacterium, one of which encodes the L. pneumophila ortholog of ahpC. Five of the clones analyzed here were fusions to promoters that were closely linked to genes encoding three-component chemiosmotic efflux pumps that export heavy metals or toxic organic compounds. Analysis of ahpC gene expression indicates that levels increased at least sevenfold during intracellular growth of the bacterium. Inactivation of several of the genes at their chromosomal loci had no effect on the intracellular growth rate of L. pneumophila in cultured macrophages. This suggests that a number of genes with increased expression during intracellular growth may be part of redundant systems that allow survival and growth under the conditions encountered within host cells.
Legionella pneumophila Putative uncharacterized protein