Gene_locus Report for: manes-HNL4

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms: N E > Eukaryota: N E > Viridiplantae: N E > Streptophyta: N E > Streptophytina: N E > Embryophyta: N E > Tracheophyta: N E > Euphyllophyta: N E > Spermatophyta: N E > Magnoliophyta: N E > Mesangiospermae: N E > eudicotyledons: N E > Gunneridae: N E > Pentapetalae: N E > rosids: N E > fabids: N E > Malpighiales: N E > Euphorbiaceae: N E > Crotonoideae: N E > Manihoteae: N E > Manihot: N E > Manihot esculenta: N E

ABHD13-BEM46 : manes-a0a2c9w6g7Manihot esculenta (Cassava) (Jatropha manihot). protein bem46-like, manes-a0a2c9ube2Manihot esculenta (Cassava) (Jatropha manihot). Protein bem46-like.
Bodyguard : manes-a0a2c9w9p1Manihot esculenta (Cassava) (Jatropha manihot) Uncharacterized protein, manes-a0a2c9wgj5Manihot esculenta (Cassava) (Jatropha manihot) Uncharacterized protein.
Glutamyl_Peptidase_S9 : manes-a0a251ksl2Manihot esculenta (Cassava) (Jatropha manihot). Peptidase_S9 domain-containing protein.
Hydroxynitrile_lyase : manes-hnl Manihot esculenta (Crantz) (Cassava) (Manioc) mRNA for alpha-hydroxynitrile lyase (MeHNL).
Plant_carboxylesterase : manes-a0a2c9uxc0Manihot esculenta (Cassava) (Jatropha manihot). Uncharacterized protein, manes-a0a2c9w3p4Manihot esculenta (Cassava) (Jatropha manihot). Abhydrolase_3 domain-containing protein, manes-a0a2c9ujw1Manihot esculenta (Cassava) (Jatropha manihot). Abhydrolase_3 domain-containing protein, manes-a0a2c9wk53Manihot esculenta (Cassava) (Jatropha manihot). Abhydrolase_3 domain-containing protein, manes-a0a2c9uly0Manihot esculenta (Cassava) (Jatropha manihot). Abhydrolase_3 domain-containing protein, manes-a0a2c9vx50Manihot esculenta (Cassava) (Jatropha manihot). Abhydrolase_3 domain-containing protein.
Plant_lipase_EDS1-like : manes-a0a2c9ujk6Manihot esculenta (Cassava) (Jatropha manihot). Uncharacterized protein, manes-a0a2c9vrv8Manihot esculenta (Cassava) (Jatropha manihot). Uncharacterized protein.
Triacylglycerol-lipase-OBL1-like : manes-a0a2c9u012Manihot esculenta (Cassava) (Jatropha manihot). Lipase_3 domain-containing protein, manes-a0a2c9usc6Manihot esculenta (Cassava) (Jatropha manihot). Lipase_3 domain-containing protein, manes-a0a2c9uws5Manihot esculenta (Cassava) (Jatropha manihot). Lipase_3 domain-containing protein, manes-a0a2c9uxf0Manihot esculenta (Cassava) (Jatropha manihot). Lipase_3 domain-containing protein, manes-a0a2c9uxi1Manihot esculenta (Cassava) (Jatropha manihot). Lipase_3 domain-containing protein, manes-a0a2c9uyh6Manihot esculenta (Cassava) (Jatropha manihot). Lipase_3 domain-containing protein, manes-a0a2c9v3j3Manihot esculenta (Cassava) (Jatropha manihot). Lipase_3 domain-containing protein, manes-a0a2c9vzn8Manihot esculenta (Cassava) (Jatropha manihot). Lipase_3 domain-containing protein, manes-a0a2c9w2t3Manihot esculenta (Cassava) (Jatropha manihot). Lipase_3 domain-containing protein.
UCP031088 : manes-a0a2c9vvl1Manihot esculenta (Cassava) (Jatropha manihot). Uncharacterized protein

Title: Genomic organization and structure of alpha-hydroxynitrile lyase in cassava (Manihot esculenta Crantz)
Hughes J, Keresztessy Z, Brown K, Suhandono S, Hughes MA
Ref: Archives of Biochemistry & Biophysics, 356:107, 1998 : PubMed
Abstract


ESTHER: Hughes_1998_Arch.Biochem.Biophys_356_107
PubMedSearch: Hughes 1998 Arch.Biochem.Biophys 356 107
PubMedID: 9705200
Gene_locus related to this paper: manes-HNL4

Abstract

Two clones with homology to the alpha-hydroxynitrile lyase (HNL) cDNA clone, MeHNL10, were isolatedfrom a lambdaEMBL3 cassava (Manihot esculenta Crantz) genomic library. Analysis of the sequences showed that both genomic clones contain HNL genes (MeHNL4, MeHNL24) which are interrupted by two introns. RT-PCR analysis of MeHNL4 shows that it is expressed at high levels in seedling roots and at lower levels in cotyledons and young leaves. The deduced amino acid sequences of MeHNL4, MeHNL10, and MeHNL24 show high sequence identity and homology to the HNL from Hevea brasiliensis whose tertiary structure has been solved at 1.9-A resolution by X-ray crystallography. This high homology allowed the construction of model structures for all of the cassava proteins using the MODELLER program. Homology modeling indicates that the short variable exon 2 encodes the "cap" region which is thought to influence the substrate specificity of the protein. Two hybrid proteins were modeled using the core alpha/beta domain of MeHNL10 and the cap region of either the Hevea HNL or a structurally related Zea protein of unknown function. This analysis suggests that changes in the active site can be engineered by swapping exons.