Gene_locus Report for: medtr-q6xsh3 Medicago truncatula (Barrel medic) lecithine cholesterol acyltransferase-like protein
Relationship
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) cellular organisms: N E > Eukaryota: N E > Viridiplantae: N E > Streptophyta: N E > Streptophytina: N E > Embryophyta: N E > Tracheophyta: N E > Euphyllophyta: N E > Spermatophyta: N E > Magnoliophyta: N E > Mesangiospermae: N E > eudicotyledons: N E > Gunneridae: N E > Pentapetalae: N E > rosids: N E > fabids: N E > Fabales: N E > Fabaceae: N E > Papilionoideae: N E > Trifolieae: N E > Medicago: N E > Medicago truncatula: N E 6_AlphaBeta_hydrolase :
medtr-q1s6d2 Medicago truncatula (Barrel medic) alpha/beta hydrolase fold .
A85-EsteraseD-FGH :
medtr-b7fj60 Medicago truncatula (Barrel medic) (Medicago tribuloides) Putative uncharacterized protein ,
medtr-b7fl49 Medicago truncatula (Barrel medic) (Medicago tribuloides) Putative uncharacterized protein .
ABHD13-BEM46 :
medtr-a0a072urp3 Medicago truncatula (Barrel medic) (Medicago tribuloides). Alpha/beta-hydrolase superfamily protein .
ABHD17-depalmitoylase :
medtr-q2hux9 Medicago truncatula (Barrel medic) esterase/lipase/thioesterase .
ABHD18 :
medtr-a7uqu0 Medicago truncatula (Barrel medic) Putative uncharacterized protein .
Acidic_Lipase :
medtr-q6tgc0 Medicago truncatula (Barrel medic) triacylglycerol/steryl ester lipase-like protein .
AlphaBeta_hydrolase :
medtr-q1s193 Medicago truncatula (Barrel medic) alpha/beta hydrolase fold ,
medtr-q1sbn0 Medicago truncatula (Barrel medic) at1g13820 ,
medtr-q1t2k5 Medicago truncatula (Barrel medic) alpha/beta hydrolase fold .
Bodyguard :
medtr-a0a072vlq4 Medicago truncatula (Barrel medic) (Medicago tribuloides) Alpha/beta-hydrolase superfamily protein .
Carboxypeptidase_S10 :
medtr-b7fki4 Medicago truncatula (Barrel medic) (Medicago tribuloides) Uncharacterized protein ,
medtr-G7ILY3 Medicago truncatula (Barrel medic) (Medicago tribuloides) peptidase s10, serine carboxypeptidase ,
medtr-G7ILY5 Medicago truncatula (Barrel medic) (Medicago tribuloides) peptidase s10, serine carboxypeptidase ,
medtr-G7IT49 Medicago truncatula (Barrel medic) (Medicago tribuloides) peptidase s10, serine carboxypeptidase ,
medtr-G7IT50 Medicago truncatula (Barrel medic) (Medicago tribuloides) peptidase s10, serine carboxypeptidase ,
medtr-g7itl1 Medicago truncatula (Barrel medic) (Medicago tribuloides) Serine carboxypeptidase ,
medtr-g7iu67 Medicago truncatula (Barrel medic) (Medicago tribuloides) Serine carboxypeptidase ,
medtr-g7j641 Medicago truncatula (Barrel medic) (Medicago tribuloides) Serine carboxypeptidase II-3 ,
medtr-g7jtf8 Medicago truncatula (Barrel medic) (Medicago tribuloides) Serine carboxypeptidase ,
medtr-g7jtg2 Medicago truncatula (Barrel medic) (Medicago tribuloides) Serine carboxypeptidase ,
medtr-g7jtg4 Medicago truncatula (Barrel medic) (Medicago tribuloides) Serine carboxypeptidase ,
medtr-g7kem3 Medicago truncatula (Barrel medic) (Medicago tribuloides) Serine carboxypeptidase-like protein ,
medtr-G7KRJ6 Medicago truncatula (Barrel medic) peptidase s10, serine carboxypeptidase ,
medtr-g7ksx5 Medicago truncatula (Barrel medic) (Medicago tribuloides) Serine carboxypeptidase-like protein ,
medtr-g7leb3 Medicago truncatula (Barrel medic) (Medicago tribuloides) Uncharacterized protein ,
medtr-q1sgx5 Medicago truncatula (Barrel medic) (Medicago tribuloides) peptidase s10, serine carboxypeptidase ,
medtr-q6w6r6 Medicago truncatula (Barrel medic) (Medicago tribuloides) putative serine carboxypeptidase ,
medtr-scp1 Medicago truncatula (Barrel medic) (Medicago tribuloides). Serine carboxypeptidase 1 .
Chlorophyllase_Plant :
medtr-b7fmi1 Medicago truncatula (Barrel medic) (Medicago tribuloides) Putative uncharacterized protein ,
medtr-g7kml3 Medicago truncatula (Barrel medic) (Medicago tribuloides). Chlorophyllase-1 ,
medtr-a0a072vhg0 Medicago truncatula (Barrel medic) (Medicago tribuloides). Chlorophyllase .
CMBL :
medtr-q1t171 Medicago truncatula (Barrel medic) (Medicago tribuloides) . Carboxymethylenebutenolidase homolog .
DPP4N_Peptidase_S9 :
medtr-g7j6w0 Medicago truncatula (Barrel medic) Peptidase S9B, dipeptidylpeptidase IV terminal .
Duf_676 :
medtr-q1s3y4 Medicago truncatula (Barrel medic) hypothetical protein ,
medtr-q1s746 Medicago truncatula (Barrel medic) (esterase/lipase/thioesterase) ,
medtr-q1spi8 Medicago truncatula (Barrel medic) Alpha/beta-hydrolase superfamily protein ,
medtr-q1su07 Medicago truncatula (Barrel medic) Serine esterase family protein, putative ,
medtr-q1szf3 Medicago truncatula (Barrel medic) Serine esterase family protein .
Duf_1350 :
medtr-a0a072trw1 Medicago truncatula (Barrel medic) (Medicago tribuloides) Plant/MCA23-20 protein ,
medtr-a0a072vdn8 Medicago truncatula (Barrel medic) (Medicago tribuloides) DUF1350 family protein ,
medtr-g7ljd0 Medicago truncatula (Barrel medic) (Medicago tribuloides), Putative uncharacterized protein .
Epoxide_hydrolase :
medtr-a2q320 Medicago truncatula (Barrel medic) (Medicago tribuloides) Epoxide hydrolase ,
medtr-a2q321 Medicago truncatula (Barrel medic) (Medicago tribuloides) Epoxide hydrolase ,
medtr-b7fjm9 Medicago truncatula (Barrel medic) (Medicago tribuloides) Putative uncharacterized protein ,
medtr-b7fjp5 Medicago truncatula (Barrel medic) (Medicago tribuloides) Putative uncharacterized protein ,
medtr-b7fka3 Medicago truncatula (Barrel medic) (Medicago tribuloides) Putative uncharacterized protein ,
medtr-q1sfz2 Medicago truncatula (Barrel medic) epoxide hydrolase (alpha/beta hydrolase fold) ,
medtr-q1sfz3 Medicago truncatula (Barrel medic) alpha/beta hydrolase fold (alpha/beta hydrolase) ,
medtr-q1sg67 Medicago truncatula (Barrel medic) alpha/beta hydrolase fold ,
medtr-q1sql8 Medicago truncatula (Barrel medic) alpha/beta hydrolase fold .
FSH1 :
medtr-q1sd74 Medicago truncatula (Barrel medic)(Medicago tribuloides); Cicer arietinum (Chickpea) (Garbanzo) hypothetical protein .
Glutamyl_Peptidase_S9 :
medtr-g7kst7 Medicago truncatula (Barrel medic) (Medicago tribuloides). Glutamyl endopeptidase, putative .
Hydroxynitrile_lyase :
medtr-b7fix6 Medicago truncatula (Barrel medic) (Medicago tribuloides) Putative uncharacterized protein ,
medtr-g7k9e1 Medicago truncatula (Barrel medic) (Medicago tribuloides) Methyl jasmonate esterase ,
medtr-g7k9e3 Medicago truncatula (Barrel medic) (Medicago tribuloides) Uncharacterized protein ,
medtr-g7k9e5 Medicago truncatula (Barrel medic) (Medicago tribuloides) Esterase PIR7B ,
medtr-g7k9e8 Medicago truncatula (Barrel medic) (Medicago tribuloides) Polyneuridine-aldehyde esterase ,
medtr-g7k9e9 Medicago truncatula (Barrel medic) (Medicago tribuloides) Pentatricopeptide repeat-containing protein ,
medtr-g7lbp2 Medicago truncatula (Barrel medic) (Medicago tribuloides) Methyl jasmonate esterase ,
medtr-g7lch3 Medicago truncatula (Barrel medic) (Medicago tribuloides) Methyl jasmonate esterase ,
medtr-i3sgp2 Medicago truncatula (Barrel medic) (Medicago tribuloides) Uncharacterized protein .
Lipase_3 :
medtr-q1sda9 Medicago truncatula (Barrel medic) lipase, class 3 .
LYsophospholipase_carboxylesterase :
medtr-q1s407 Medicago truncatula (Barrel medic) phospholipase/carboxylesterase .
Monoglyceridelipase_lysophospholip :
medtr-q1rus1 Medicago truncatula (Barrel medic) alpha/beta hydrolase ,
medtr-q1s4m1 Medicago truncatula (Barrel medic) alpha/beta hydrolase .
Ndr_family :
medtr-g7ib94 Medicago truncatula (Barrel medic) (Medicago tribuloides) Pollen-specific protein SF21 ,
medtr-g7ljk8 Medicago truncatula (Barrel medic) (Medicago tribuloides) Pollen specific protein SF21 ,
medtr-g7i6w5 Medicago truncatula (Barrel medic) (Medicago tribuloides) Pollen-specific protein SF21 .
PC-sterol_acyltransferase :
medtr-q1s687 Medicago truncatula (Barrel medic) lecithin:cholesterol acyltransferase ,
medtr-Q8GZM5 Medicago truncatula (Barrel medic) (Medicago tribuloides) putative phosphatidylcholine acyltransferase ,
medtr-q71ce5 Medicago truncatula (Barrel medic) lecithine cholesterol acyltransferase-like protein .
Pectinacetylesterase-Notum :
medtr-b7fkl0 Medicago truncatula (Barrel medic) (Medicago tribuloides) Putative uncharacterized protein ,
medtr-q2hru2 Medicago truncatula (Barrel medic) (Medicago tribuloides) Pectinacetylesterase ,
medtr-g7kvg4 Medicago truncatula (Barrel medic) (Medicago tribuloides). Pectinacetylesterase family protein ,
medtr-g7iam1 Medicago truncatula (Barrel medic) (Medicago tribuloides). Pectinacetylesterase family protein ,
medtr-g7iam3 Medicago truncatula (Barrel medic) (Medicago tribuloides). Pectinacetylesterase family protein ,
medtr-g7l754 Medicago truncatula (Barrel medic) (Medicago tribuloides). Pectinacetylesterase family protein ,
medtr-g7jr41 Medicago truncatula (Barrel medic) (Medicago tribuloides). Pectinacetylesterase family protein ,
medtr-g7l4f5 Medicago truncatula (Barrel medic) (Medicago tribuloides). Pectinacetylesterase family protein ,
medtr-i3scp6 Medicago truncatula (Barrel medic) (Medicago tribuloides). Uncharacterized protein ,
medtr-g7l755 Medicago truncatula (Barrel medic) (Medicago tribuloides). Pectinacetylesterase family protein ,
medtr-a0a072vyl4 Medicago truncatula (Barrel medic) (Medicago tribuloides). Pectinacetylesterase family protein ,
medtr-g7jwk8 Medicago truncatula (Barrel medic) (Medicago tribuloides). Pectinacetylesterase family protein .
Pheophytinase :
medtr-g7izm0 Medicago truncatula (Barrel medic) (Medicago tribuloides) Alpha/beta hydrolase-fold family protein .
Plant_carboxylesterase :
medtr-q1s1e6 Medicago truncatula (Barrel medic) esterase/lipase/thioesterase ,
medtr-q1s5d8 Medicago truncatula (Barrel medic) (Medicago tribuloides) alpha/beta hydrolase fold, putative (esterase/lipase/thioesterase) ,
medtr-q1smf6 Medicago truncatula (Barrel medic) esterase/lipase/thioesterase ,
medtr-q1smf7 Medicago truncatula (Barrel medic) esterase/lipase/thioesterase ,
medtr-q1szi1 Medicago truncatula (Barrel medic) lipolytic enzyme ,
medtr-q1szi4 Medicago truncatula (Barrel medic) esterase/lipase/thioesterase ,
medtr-q1szi7 Medicago truncatula (Barrel medic) esterase/lipase/thioesterase ,
medtr-q1szi9 Medicago truncatula (Barrel medic) esterase/lipase/thioesterase ,
medtr-q2hrx9 Medicago truncatula (Barrel medic) esterase/lipase/thioesterase ,
medtr-a0a072uuf6 Medicago truncatula (Barrel medic) (Medicago tribuloides). Putative carboxylesterase ,
medtr-g7zzc3 Medicago truncatula (Barrel medic) (Medicago tribuloides). Putative carboxylesterase ,
medtr-g7ie19 Medicago truncatula (Barrel medic) (Medicago tribuloides). 2-hydroxyisoflavanone dehydratase .
Plant_lipase_EDS1-like :
medtr-q1s9m3 Medicago truncatula (Barrel medic) Enhanced disease susceptibility protein ,
medtr-a0a072v056 Medicago truncatula (Barrel medic) (Medicago tribuloides).. Enhanced disease susceptibility protein .
Proline_iminopeptidase :
medtr-b7fl89 Medicago truncatula (Barrel medic) (Medicago tribuloides). Putative uncharacterized protein .
RsbQ-like :
medtr-MtD14 Medicago truncatula (Barrel medic) (Medicago tribuloides) D14 Sigma factor sigb regulation rsbq-like protein Medtr1g018320 ,
medtr-b7fk41 Medicago truncatula (Barrel medic) (Medicago tribuloides) KAI2A Putative alpha/Beta hydrolase, strigolactone esterase Medtr5g016150 ,
medtr-q1sed4 Medicago truncatula (Barrel medic) KAI2B Medtr4g095310 alpha/beta hydrolase fold .
Triacylglycerol-lipase-OBL1-like :
medtr-q1s2a7 Medicago truncatula (Barrel medic)(Medicago tribuloides) lipase, putative ,
medtr-q1sh82 Medicago truncatula (Barrel medic)(Medicago tribuloides) lipase, class 3 ,
medtr-a0a072u5k5 Medicago truncatula (Barrel medic) (Medicago tribuloides). Triacylglycerol lipase-like protein .
UCP031088 :
medtr-a0a072vrv9 Medicago truncatula (Barrel medic) (Medicago tribuloides). Alpha/beta fold hydrolase ,
medtr-g7kmk5 Medicago truncatula (Barrel medic) (Medicago tribuloides). Putative alpha/Beta hydrolase Molecular evidence
Database
No mutation No structure No kinetic No Substrate No inhibitor
Sequence
Graphical view for this peptide sequence: medtr-q6xsh3 Colored MSA for PC-sterol_acyltransferase (raw)
MALIRRRKGSEPEKGQSENSEPKIQKKEDEDDDNDKNKKNKKKNKDEVGV
FKKSTRWSCVDSCCWFVGCICTLWWFLLFMYNVMPASFPQYVTEAITGPM
PDPPGLKLKKEGLSVKHPVVFVPGIVTGGLELWEGHQCAEGLFRKRLWGG
TFGEVYKRPSCWVEHMSLDNETGMDPPGITVRPVSGLVAADYFAAGYFVW
ADLIANLARIGYEEKTMYMAAYDWRISFQNTEVRDQTLSRIKSNIELMVS
TNGGNKAVIIPHSMGVLYFLHFMKWVEAPAPMGGGGGPDWCSKYIKAIVN
IGGPFLGVPKAIAGLFSAEARDIAVARAIAPGFLDNDMFRIQTLQHVMKM
TRTWDSTMSMIPKGGDTIWGDLDWSPEESCGLHTRKQSSNNTQLTEQKTN
KTNVNYGRMISFGKAVAEADSSKIDIVDFRGAIKGPNVANTSCRDVWTEY
HDMGVEGVRAVAAVAEHKVYTAGSIIDLLQFVAPKMMARGSDHFSYGVAD
NLDDPKYEHYKYWSNPLETKLPNAPDMEIFSLYGVGLPTERAYIYKLTPF
AECYIPFEIDPNAEGGDKVSCLKDGVYTVDGDETVPVLSSGYMCAKGWRG
KTRFNPSGIRTYVREYDHSPPANLLEGRGTQSGAHVDIMGNFALIEDVMR
VAAGAKGEELGGDKVYSDIFKWSDRIKLPL
Legend
This sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA
M A L I R R R K G S E P E K G Q S E N S E P K I Q K K E D E D D D N D K N K K N K K K N K D E V G V F K K S T R W S C V D S C C W F V G C I C T L W W F L L F M Y N V M P A S F P Q Y V T E A I T G P M P D P P G L K L K K E G L S V K H P V V F V P G I V T G G L E L W E G H Q C A E G L F R K R L W G G T F G E V Y K R P S C W V E H M S L D N E T G M D P P G I T V R P V S G L V A A D Y F A A G Y F V W A D L I A N L A R I G Y E E K T M Y M A A Y D W R I S F Q N T E V R D Q T L S R I K S N I E L M V S T N G G N K A V I I P H S M G V L Y F L H F M K W V E A P A P M G G G G G P D W C S K Y I K A I V N I G G P F L G V P K A I A G L F S A E A R D I A V A R A I A P G F L D N D M F R I Q T L Q H V M K M T R T W D S T M S M I P K G G D T I W G D L D W S P E E S C G L H T R K Q S S N N T Q L T E Q K T N K T N V N Y G R M I S F G K A V A E A D S S K I D I V D F R G A I K G P N V A N T S C R D V W T E Y H D M G V E G V R A V A A V A E H K V Y T A G S I I D L L Q F V A P K M M A R G S D H F S Y G V A D N L D D P K Y E H Y K Y W S N P L E T K L P N A P D M E I F S L Y G V G L P T E R A Y I Y K L T P F A E C Y I P F E I D P N A E G G D K V S C L K D G V Y T V D G D E T V P V L S S G Y M C A K G W R G K T R F N P S G I R T Y V R E Y D H S P P A N L L E G R G T Q S G A H V D I M G N F A L I E D V M R V A A G A K G E E L G G D K V Y S D I F K W S D R I K L P L
Reference
Title: Expression in yeast of a novel phospholipase A1 cDNA from Arabidopsis thalianaNoiriel A , Benveniste P , Banas A , Stymne S , Bouvier-Nave P Ref: European Journal of Biochemistry, 271 :3752, 2004 : PubMed Abstract ESTHER: Noiriel_2004_Eur.J.Biochem_271_3752 PubMedSearch: Noiriel 2004 Eur.J.Biochem 271 3752 PubMedID: 15355352 Gene_locus related to this paper: arath-At5g13640 ,
arath-LCAT1 ,
arath-LCAT4 ,
arath-LCAT3 ,
lyces-q71lx7 ,
medtr-q6xsh3 ,
medtr-Q8GZM5 ,
medtr-q71ce5 ,
tobac-q71lw1 Abstract
During a search for cDNAs encoding plant sterol acyltransferases, we isolated four full-length cDNAs from Arabidopsis thaliana that encode proteins with substantial identity with animal lecithin : cholesterol acyltransferases (LCATs). The expression of one of these cDNAs, AtLCAT3 (At3g03310), in various yeast strains resulted in the doubling of the triacylglycerol content. Furthermore, a complete lipid analysis of the transformed wild-type yeast showed that its phospholipid content was lower than that of the control (void plasmid-transformed) yeast whereas lysophospholipids and free fatty acids increased. When microsomes from the AtLCAT3-transformed yeast were incubated with di-[1-14C]oleyl phosphatidylcholine, both the lysophospholipid and free fatty acid fractions were highly and similarly labelled, whereas the same incubation with microsomes from the control yeast produced a negligible labelling of these fractions. Moreover when microsomes from AtLCAT3-transformed yeast were incubated with either sn-1- or sn-2-[1-14C]acyl phosphatidylcholine, the distribution of the labelling between the free fatty acid and the lysophosphatidylcholine fractions strongly suggested a phospholipase A1 activity for AtLCAT3. The sn-1 specificity of this phospholipase was confirmed by gas chromatography analysis of the hydrolysis of 1-myristoyl, 2-oleyl phosphatidylcholine. Phosphatidylethanolamine and phosphatidic acid were shown to be also hydrolysed by AtLCAT3, although less efficiently than phosphatidylcholine. Lysophospatidylcholine was a weak substrate whereas tripalmitoylglycerol and cholesteryl oleate were not hydrolysed at all. This novel A. thaliana phospholipase A1 shows optimal activity at pH 6-6.5 and 60-65 degrees C and appears to be unaffected by Ca2+. Its sequence is unrelated to all other known phospholipases. Further studies are in progress to elucidate its physiological role.
Other Papers
