(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Opisthokonta: NE > Metazoa: NE > Eumetazoa: NE > Bilateria: NE > Protostomia: NE > Lophotrochozoa: NE > Mollusca: NE > Bivalvia: NE > Pteriomorphia: NE > Pectinoida: NE > Pectinoidea: NE > Pectinidae: NE > Mizuhopecten: NE > Mizuhopecten yessoensis: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MAAFLRLLSLFILSKITTGFLFTRQVCYQHIGCFSNARPFRNAKGYLPEP PDHIQTNFLLYTRQNTTHAKSLDPYNNATVTGSHFDSAKNTKVIIHGYRD SGHSHWMQQMVQVFLNTEDVNVIAVDWSLGADNINYIKSAANTRVVGATT AKLLEQLHHTTGLSYSRVHLIGHSLGSHIAGYAGRRVHGIGRITGLDPAG PLFENFDAQVRLDPTDASFVDVIHSDSDSLSKLGFGLDKALGHADFYPNG GEKQPGCSQEDVNHWFFLIALQIEQFTDTVACSHMRAIALFTESIPTSGC SFTAYPCQSKADYDAGRCHSCDQGCSEMGYHADKYSAHGKFYLSTTGSPP FCQ
Reconstructing the genomes of bilaterian ancestors is central to our understanding of animal evolution, where knowledge from ancient and/or slow-evolving bilaterian lineages is critical. Here we report a high-quality, chromosome-anchored reference genome for the scallop Patinopecten yessoensis, a bivalve mollusc that has a slow-evolving genome with many ancestral features. Chromosome-based macrosynteny analysis reveals a striking correspondence between the 19 scallop chromosomes and the 17 presumed ancestral bilaterian linkage groups at a level of conservation previously unseen, suggesting that the scallop may have a karyotype close to that of the bilaterian ancestor. Scallop Hox gene expression follows a new mode of subcluster temporal co-linearity that is possibly ancestral and may provide great potential in supporting diverse bilaterian body plans. Transcriptome analysis of scallop mantle eyes finds unexpected diversity in phototransduction cascades and a potentially ancient Pax2/5/8-dependent pathway for noncephalic eyes. The outstanding preservation of ancestral karyotype and developmental control makes the scallop genome a valuable resource for understanding early bilaterian evolution and biology.
Title: Pancreatic lipase-related protein (PY-PLRP) highly expressed in the vitellogenic ovary of the scallop, Patinopecten yessoensis Kim KS, Kim BK, Kim HJ, Yoo MS, Mykles DL, Kim HW Ref: Comparative Biochemistry & Physiology B Biochem Mol Biol, 151:52, 2008 : PubMed
A cDNA (1206 bp) encoding a pancreatic lipase-related protein (PY-PLRP) was obtained from the ovary of the scallop, Patinopecten yessoensis, using a differentially expressed gene system. The open reading frame specified a protein containing 353 amino acids (~38 kDa). The N-terminal catalytic domain, which contained the catalytic triad of serine, aspartate, and histidine residues, 10 cysteine residues involved in disulfide bridges, and the conserved lid domain, indicated that the protein would be catalytically active. However, PY-PLRP lacked the C-terminal colipase-binding domain present in mammalian PLRPs. Sequence analysis of the catalytic domains of PY-PLRP with members of the pancreatic triglyceride lipase (PTL) family suggested that PY-PLRP was related to mammalian PLRP1, PLRP2, and PTL. End-point reverse transcriptase-polymerase chain reaction (RT-PCR) after 25 cycles showed that PY-PLRP was expressed at a high level in the ovary and at low levels in testis and mantle; expression was not detected in gill, digestive gland, and adductor muscle. Quantitative PCR of RNA from ovaries at different stages of development showed that PY-PLRP was expressed at a significantly higher level in the late growth stage than in the ripe and spent stages. These data suggest that PY-PLRP plays a role in lipid metabolism associated with oocyte maturation and vitellogenesis that occurs during ovarian growth.
