Gene_locus Report for: mycs2-a0r619 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MSMEG_6394 Putative uncharacterized protein
Relationship
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) cellular organisms: N E > Bacteria: N E > Terrabacteria group: N E > Actinobacteria [phylum]: N E > Actinobacteria [class]: N E > Corynebacteriales: N E > Mycobacteriaceae: N E > Mycobacterium: N E > Mycobacterium smegmatis: N E 6_AlphaBeta_hydrolase :
mycs2-a0qnp5 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Hydrolase ,
mycs2-a0qpn5 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) alpha/beta hydrolase fold ,
mycs2-a0qrg4 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) hydrolase, alpha/beta fold family protein ,
mycs2-a0qsr7 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) alpha/beta hydrolase fold ,
mycs2-a0qsz4 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Hydrolase, alpha/beta fold family protein, putative ,
mycs2-a0qtr8 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) hydrolase, alpha/beta fold family protein ,
mycs2-a0qu09 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) hydrolase, alpha/beta fold family protein ,
mycs2-a0qvd3 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Putative carboxylesterase protein ,
mycs2-a0qwl5 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) putative hydrolase ,
mycs2-a0qxh0 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) transcriptional regulator, cadc ,
mycs2-a0qxk5 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) hydrolase ,
mycs2-a0qz09 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) transcriptional regulator, cadc ,
mycs2-a0qzm4 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) putative hydrolase ,
mycs2-a0qzz2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Uncharacterized protein ,
mycs2-a0r0r2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) hydrolase, N-terminal alpha/beta-hydrolase domain and a C-terminal cyclase homology domain ,
mycs2-a0r2g9 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Secreted protein ,
mycs2-a0r2r7 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Hydrolase, alpha/beta fold family protein ,
mycs2-a0r2r9 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Hydrolase ,
mycs2-a0r3z7 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) alpha/beta hydrolase fold ,
mycs2-a0r4a4 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Putative uncharacterized protein ,
mycs2-a0r5d4 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Alpha/beta hydrolase fold-1 ,
mycs2-a0r6f8 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) putative uncharacterized protein ,
mycs2-a0r6s8 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) alpha/beta hydrolase fold ,
mycs2-a0r7g7 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Putative hydrolase ,
mycs2-a0r262 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) hydrolase, alpha/beta fold family protein ,
mycs2-a0r568 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) hydrolase, alpha/beta hydrolase fold family protein ,
mycs2-a0r733 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Oxidoreductase ,
mycs2-a0r797 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) hydrolase, alpha/beta hydrolase fold family protein .
A85-Est-Putative :
mycs2-a0qr95 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Putative esterase family protein ,
mycs2-a0qyv2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Putative esterase family protein .
A85-Mycolyl-transferase :
mycs2-a0qu51 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Antigen 85-C ,
mycs2-a0qxv3 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Putative esterase family protein ,
mycs2-a0qy95 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Antigen 85-C ,
mycs2-a0r6k6 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Antigen 85-C ,
mycs2-a0r621 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Antigen 85-C ,
mycs2-a0r623 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Antigen 85-A ,
mycs2-a0r624 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Antigen 85-C .
Abhydrolase_9 :
mycs2-a0r2y6 Mycobacterium smegmatis, Conserved membrane-spanning protein ,
mycs2-a0r4g3 Mycobacterium smegmatis Conserved membrane-spanning protein .
Aclacinomycin-methylesterase_RdmC :
mycs2-a0qs51 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) hydrolase, alpha/beta fold family protein .
AlphaBeta_hydrolase :
mycs2-a0qsr0 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) carboxylesterase .
BD-FAE :
mycs2-a0qp66 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) alpha/beta hydrolase fold family protein ,
mycs2-a0qp80 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) peptidase, s9a/b/c families (EC 3.4.-.-) .
Carbon-carbon_bond_hydrolase :
mycs2-a0qvb5 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Hydrolase, alpha/beta fold family protein, putative ,
mycs2-a0qwd3 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase ,
mycs2-a0r1s8 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Hydrolase, alpha/beta fold family protein, putative ,
mycs2-a0r1t5 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) 2-hydroxy-6-ketonona-2,4-dienedoic acid hydrolase ,
mycs2-a0r6l0 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Alpha/beta hydrolase, putative ,
mycs2-a0r523 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) 2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase .
Carboxymethylbutenolide_lactonase :
mycs2-a0qtm5 Mycobacterium smegmatis 3-oxoadipate enol-lactonase (EC 3.1.1.24) Adhesin .
Carb_B_Bacteria :
mycs2-a0qpp6 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) para-nitrobenzyl esterase (EC 3.1.1.-) ,
mycs2-a0qq34 Mycobacterium smegmatis para-nitrobenzyl esterase (EC 3.1.1.-) ,
mycs2-a0qsc7 Mycobacterium smegmatis esterase (EC 3.1.1.7) ,
mycs2-a0qwn1 Mycobacterium smegmatis para-nitrobenzyl esterase (EC 3.1.1.-) ,
mycs2-a0qyi2 Mycobacterium smegmatis para-nitrobenzyl esterase (EC 3.1.1.-) ,
mycs2-a0qz01 Mycobacterium smegmatis para-nitrobenzyl esterase (EC 3.1.1.-) ,
mycs2-a0r5t4 Mycobacterium smegmatis para-nitrobenzyl esterase (EC 3.1.1.-) ,
mycsm-a0a0d6gfu5 Mycobacterium smegmatis. Carboxylic ester hydrolase .
CFTR-inhibitory-factor_Cif :
mycs2-a0r6y9 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Epoxide hydrolase ,
mycs2-a0r6z0 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Epoxide hydrolase .
Cutinase :
mycs2-a0qnx2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Serine esterase, cutinase family protein ,
mycs2-a0qsm2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Cutinase Cut4 ,
mycs2-a0r5y1 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Serine esterase, cutinase family protein ,
mycs2-a0qsa0 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Cutinase superfamily protein ,
mycs2-a0r4l6 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Serine esterase, cutinase family protein ,
mycs2-a0qsm3 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Serine esterase, cutinase family protein ,
mycs2-a0qu66 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Serine esterase, cutinase family protein ,
mycs2-a0r0q0 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Cutinase Cut1 ,
mycs2-a0qv75 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Probable cutinase Cut3 ,
mycs2-a0qrn8 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Serine esterase, cutinase family protein ,
mycs2-a0qsm1 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Putative cutinase cut2 .
Duf_1023 :
mycs2-a0qpe0 Mycobacterium smegmatis, Mycobacterium goodii, Uncharacterized protein ,
mycs2-a0r606 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Uncharacterized protein .
Epoxide_hydrolase :
mycs2-a0qnw0 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Epoxide hydrolase 1 ,
mycs2-a0qtx4 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Hydrolase, alpha/beta fold family protein ,
mycs2-a0qwe5 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Hydrolase ,
mycs2-a0qxg0 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Hydrolase, alpha/beta fold family protein ,
mycs2-a0qxs4 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Epoxide hydrolase ,
mycs2-a0r5g5 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Hydrolase, alpha/beta fold family protein ,
mycs2-a0r070 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Short chain dehydrogenase ,
mycs2-a0r403 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Epoxide hydrolase ,
mycs2-a0r589 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Epoxide hydrolase .
Fungal-Bact_LIP :
mycs2-a0quy5 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Putative uncharacterized protein ,
mycs2-a0qx73 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Triacylglycerol lipase .
Haloacetate_dehalogenase :
mycs2-a0qtw0 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Haloacetate dehalogenase H-1 ,
mycs2-a0r6x8 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Epoxide hydrolase .
Haloalkane_dehalogenase-HLD1 :
mycs2-a0r5u5 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Haloalkane dehalogenase 1 .
Haloalkane_dehalogenase-HLD2 :
mycsm-Q938B4 Mycobacterium smegmatis putative linb haloalkane dehalogenase (fragment) .
Haloperoxidase :
mycs2-a0qxg2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) gp61 protein ,
mycs2-a0r1c9 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) non-heme bromoperoxidase bpo-a2 (EC 1.11.1.-) .
HNLyase_Bact :
mycs2-a0qtd1 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) salicylate esterase ,
mycs2-a0qyz5 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Putative esterase ,
mycs2-a0r6y0 .
Homoserine_transacetylase :
mycs2-metx .
Homoserine_transacetylase_like_est :
mycs2-i7gg46 Mycobacterium smegmatis Homoserine O-acetyltransferase MSMEG_6772 .
Hormone-sensitive_lipase_like :
mycsm-Q938C6 Mycobacterium smegmatis putative carboxylesterase/lipase ,
mycs2-a0qwt7 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155). Esterase Lipase LipH .
MEST-like :
mycs2-a0qw20 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) alpha/beta hydrolase fold .
Monoglyceridelipase_lysophospholip :
mycs2-a0qnz7 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Monoglyceride lipase ,
mycs2-a0qvl1 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Putative uncharacterized protein ,
mycs2-a0r515 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Putative uncharacterized protein .
NLS3-Tex30 :
mycs2-a0qqp0 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Uncharacterized protein .
PE-PPE :
mycs2-a0qrn0 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Putative uncharacterized protein ,
mycs2-a0qvy2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Ppe family protein ,
mycs2-a0r1f5 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Putative uncharacterized protein ,
mycs2-a0r3f6 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Pe family protein ,
mycs2-a0r3k1 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Putative uncharacterized protein ,
mycs2-a0r3k2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Putative uncharacterized protein ,
mycs2-a0r4a0 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Pe family protein ,
mycs2-a0r5c7 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Pe family protein ,
mycs2-a0r356 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) PPE family protein, putative ,
mycs2-q2m5k2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Putative uncharacterized protein .
PHA_synth_III_C :
mycs2-a0qys3 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Acyl-CoA synthase .
Proline_iminopeptidase :
mycs2-a0qvs8 .
Thioesterase :
mycsm-FXBC Mycobacterium smegmatis fxbc .
Tiancimycin-TnmK-Tripeptidase-HIP :
mycs2-a0r084 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Hydrolase, alpha/beta fold family protein ,
mycs2-a0r085 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Protease Molecular evidence
Database
No mutation 1 structure :
3AJA : Crystal structure of Mycobacterium smegmatis MSMEG_6394 orthologue of Rv3802c
No kinetic 1 substrate :
Cutin 1 inhbitor :
Orlistat
Sequence
Graphical view for this peptide sequence: mycs2-a0r619 Colored MSA for Cutinase (raw)
MAKNARRKRHRILALIAAAAMALVVVLVVTIVVVIMRRPDTPATPPPSAE
PPGGVVVPPGTRKPRPEFQSADCPDVMMVSIPGTWESSPTDDPFNPTQFP
LSLMSNISKPLAEQFGPDRLQVYTTPYTAQFHNPFAADKQMSYNDSRAEG
MRTTVKAMTDMNDRCPLTSYVIAGFSQGAVIAGDIASDIGNGRGPVDEDL
VLGVTLIADGRRQMGVGQDVGPNPAGQGAEITLHEVPALSALGLTMTGPR
PGGFGALDNRTNQICGSGDLICSAPEQAFSVFNLPKTLETLSGSAAGPVH
ALYNTPQFWVENGQTATQWTLEWARNLVENAPHPKHG
Legend
This sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA
M A K N A R R K R H R I L A L I A A A A M A L V V V L V V T I V V V I M R R P D T P A T P P P S A E P P G G V V V P P G T R K P R P E F Q S A D C P D V M M V S I P G T W E S S P T D D P F N P T Q F P L S L M S N I S K P L A E Q F G P D R L Q V Y T T P Y T A Q F H N P F A A D K Q M S Y N D S R A E G M R T T V K A M T D M N D R C P L T S Y V I A G F S Q G A V I A G D I A S D I G N G R G P V D E D L V L G V T L I A D G R R Q M G V G Q D V G P N P A G Q G A E I T L H E V P A L S A L G L T M T G P R P G G F G A L D N R T N Q I C G S G D L I C S A P E Q A F S V F N L P K T L E T L S G S A A G P V H A L Y N T P Q F W V E N G Q T A T Q W T L E W A R N L V E N A P H P K H G
Reference
Title: Tetrahydrolipstatin inhibition, functional analyses, and three-dimensional structure of a lipase essential for mycobacterial viabilityCrellin PK , Vivian JP , Scoble J , Chow FM , West NP , Brammananth R , Proellocks NI , Shahine A , Le Nours J and Beddoe T <4 more author(s)> Crellin PK , Vivian JP , Scoble J , Chow FM , West NP , Brammananth R , Proellocks NI , Shahine A , Le Nours J , Wilce MC , Britton WJ , Coppel RL , Rossjohn J , Beddoe T (- 4) Ref: Journal of Biological Chemistry, 285 :30050, 2010 : PubMed Abstract ESTHER: Crellin_2010_J.Biol.Chem_285_30050 PubMedSearch: Crellin 2010 J.Biol.Chem 285 30050 PubMedID: 20656688 Gene_locus related to this paper: mycs2-a0r619 Inhibitor(s) related to this paper: Orlistat Abstract
The highly complex and unique mycobacterial cell wall is critical to the survival of Mycobacteria in host cells. However, the biosynthetic pathways responsible for its synthesis are, in general, incompletely characterized. Rv3802c from Mycobacterium tuberculosis is a partially characterized phospholipase/thioesterase encoded within a genetic cluster dedicated to the synthesis of core structures of the mycobacterial cell wall, including mycolic acids and arabinogalactan. Enzymatic assays performed with purified recombinant proteins Rv3802c and its close homologs from Mycobacterium smegmatis (MSMEG_6394) and Corynebacterium glutamicum (NCgl2775) show that they all have significant lipase activities that are inhibited by tetrahydrolipstatin, an anti-obesity drug that coincidently inhibits mycobacterial cell wall biosynthesis. The crystal structure of MSMEG_6394, solved to 2.9 A resolution, revealed an alpha/beta hydrolase fold and a catalytic triad typically present in esterases and lipases. Furthermore, we demonstrate direct evidence of gene essentiality in M. smegmatis and show the structural consequences of loss of MSMEG_6394 function on the cellular integrity of the organism. These findings, combined with the predicted essentiality of Rv3802c in M. tuberculosis, indicate that the Rv3802c family performs a fundamental and indispensable lipase-associated function in mycobacteria.
Other Papers
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MSMEG_6394 Putative uncharacterized protein
