(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Opisthokonta: NE > Fungi: NE > Dikarya: NE > Ascomycota: NE > saccharomyceta: NE > Saccharomycotina: NE > Saccharomycetes: NE > Saccharomycetales: NE > Pichiaceae: NE > Ogataea: NE > Ogataea parapolymorpha: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MGQQVSTQGTTPYSVAIPGHGTLTGYTFKAPRTGNSSVHRFAKVPFAKPS TPENRFKLPEPLPSDYDYTGDYKDFGLKCPQPSVPHPQFRYTKSPTEENI LYSNIWVPASDKYKPKDGWPVLIYIHGGFLQYSTPNNEMFNVHEMFDDED FTQKFILVAPGYRLNMFGFLSSKELLEEDAKCSNQGFWDQRLAIEWTYKN IKHFGGNPDKITVGGISAGAYSAFFQLAYELYHPEAEQIIKQAMFFSNMV YIQPKTIAEVQNQFDEIIEKLGIDPNASSAEKLQKLRSIDTGFIEDFIPT LSLHTFRAVTDGHFISPDLIKDIRSGKHAKMLAQKGVRILIGEVDNELVK YSYLNTPQTVSELPLQVQNYYPKVVVPTLMDLYGAEKLDENSPDFKEDLR KVYGAIISDGQVYASARGYINSLVQNGYPEKNIYRYRISFRAKWLDQHIS PDLNVPHACDAHLWFYCLREGYTEPERLVINKWLQPYLQFVNFEQDITEW PSFDAKKIRLLKRDGQAEYVEDPDWDWGVKVATAVYDKQIECTT
BACKGROUND: Hansenula polymorpha DL1 is a methylotrophic yeast, widely used in fundamental studies of methanol metabolism, peroxisome biogenesis and function, and also as a microbial cell factory for production of recombinant proteins and metabolic engineering towards the goal of high temperature ethanol production. RESULTS: We have sequenced the 9 Mbp H. polymorpha DL1 genome and performed whole-genome analysis for the H. polymorpha transcriptome obtained from both methanol- and glucose-grown cells. RNA-seq analysis revealed the complex and dynamic character of the H. polymorpha transcriptome under the two studied conditions, identified abundant and highly unregulated expression of 40% of the genome in methanol grown cells, and revealed alternative splicing events. We have identified subtelomerically biased protein families in H. polymorpha, clusters of LTR elements at G + C-poor chromosomal loci in the middle of each of the seven H. polymorpha chromosomes, and established the evolutionary position of H. polymorpha DL1 within a separate yeast clade together with the methylotrophic yeast Pichia pastoris and the non-methylotrophic yeast Dekkera bruxellensis. Intergenome comparisons uncovered extensive gene order reshuffling between the three yeast genomes. Phylogenetic analyses enabled us to reveal patterns of evolution of methylotrophy in yeasts and filamentous fungi. CONCLUSIONS: Our results open new opportunities for in-depth understanding of many aspects of H. polymorpha life cycle, physiology and metabolism as well as genome evolution in methylotrophic yeasts and may lead to novel improvements toward the application of H. polymorpha DL-1 as a microbial cell factory.
