(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Opisthokonta: NE > Fungi: NE > Dikarya: NE > Ascomycota: NE > saccharomyceta: NE > Pezizomycotina: NE > leotiomyceta: NE > Eurotiomycetes: NE > Eurotiomycetidae: NE > Onygenales: NE > Onygenales incertae sedis: NE > Paracoccidioides: NE > Paracoccidioides brasiliensis: NE
Warning: This entry is a compilation of different species or line or strain with more than 90% amino acide identity. You can retrieve all strain data
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) Paracoccidioides brasiliensis Pb03: N, E.
Paracoccidioides brasiliensis Pb18: N, E.
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MKSLVLGLLVGSAIASGPLQHVLHAPPDPEPKPEPEPQVVKDPFEELRDT FDRLRNKAGDIWDDVMDNIPNIMSNMRPLTIPAKKFTRRPDSEWTHIVRG ADLEALWVDDESGYKHRKIDGKLAQYDLRIKAVDPSDLGIDKVKQYSGYL DDNANDKHLFFWFFESRNDPFGDPVVLWLNGGPGCSSLTGMFFELGPASI DENITANYNPYSWNSNSSIIFLDQPVNVGYSYSSQAVSDTVTAAKDVYAL LTLFFTQFRQYSAQDFHIAGESYAGHYIPVFASEILHHNNTNINLQSVLI GNGLTDPLSQYPFYRPMACGDGGYPSVLDSQSCQSMDNALPRCLSMIKSC YDIESTFTCLPASIYCNNALIGPYQKTGRNPYDVRTNCTGNDLCYPQLNY ITEYLNKPHVMRSLGVEVDSYESCNMDINRNFLFHGDWMKPYHRLVPSLL ARIPVLIYAGDADFICNWLGNKAWTEALEYPGHAKFAEAPMENLTMINSQ GKNEVFGEVKSHSNLTFMRIFKAGHMTPFDSPQASLEFANSWLSGEWSEV
Paracoccidioides is a fungal pathogen and the cause of paracoccidioidomycosis, a health-threatening human systemic mycosis endemic to Latin America. Infection by Paracoccidioides, a dimorphic fungus in the order Onygenales, is coupled with a thermally regulated transition from a soil-dwelling filamentous form to a yeast-like pathogenic form. To better understand the genetic basis of growth and pathogenicity in Paracoccidioides, we sequenced the genomes of two strains of Paracoccidioides brasiliensis (Pb03 and Pb18) and one strain of Paracoccidioides lutzii (Pb01). These genomes range in size from 29.1 Mb to 32.9 Mb and encode 7,610 to 8,130 genes. To enable genetic studies, we mapped 94% of the P. brasiliensis Pb18 assembly onto five chromosomes. We characterized gene family content across Onygenales and related fungi, and within Paracoccidioides we found expansions of the fungal-specific kinase family FunK1. Additionally, the Onygenales have lost many genes involved in carbohydrate metabolism and fewer genes involved in protein metabolism, resulting in a higher ratio of proteases to carbohydrate active enzymes in the Onygenales than their relatives. To determine if gene content correlated with growth on different substrates, we screened the non-pathogenic onygenale Uncinocarpus reesii, which has orthologs for 91% of Paracoccidioides metabolic genes, for growth on 190 carbon sources. U. reesii showed growth on a limited range of carbohydrates, primarily basic plant sugars and cell wall components; this suggests that Onygenales, including dimorphic fungi, can degrade cellulosic plant material in the soil. In addition, U. reesii grew on gelatin and a wide range of dipeptides and amino acids, indicating a preference for proteinaceous growth substrates over carbohydrates, which may enable these fungi to also degrade animal biomass. These capabilities for degrading plant and animal substrates suggest a duality in lifestyle that could enable pathogenic species of Onygenales to transfer from soil to animal hosts.
