(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Viridiplantae: NE > Streptophyta: NE > Streptophytina: NE > Embryophyta: NE > Tracheophyta: NE > Euphyllophyta: NE > Spermatophyta: NE > Magnoliophyta: NE > Mesangiospermae: NE > eudicotyledons: NE > Gunneridae: NE > Pentapetalae: NE > rosids: NE > fabids: NE > Fabales: NE > Fabaceae: NE > Papilionoideae: NE > Phaseoleae: NE > Vigna: NE > Vigna angularis: NE > Vigna angularis var. angularis: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MAEKEAIEEAHSKLRFKLLREILPPKVRHYRKVVSPYYENSPPTKEEMEI MESCPKVDRENLEELLQEENFYLVMNWNQGSESGDEDVKSRRRIPWVPVL IYKPKESDNHKKRPAVVFLHDMNKNKEFFRPLLKAYASRGYIAVAVDACY HGERAMYTHAYRDVCDLMRLADYLTEREDIDPSRIGITGISLGGIHAWFA AVADTRYSVVVPLIAVRFIAEPGVGHQLTTFQLKESADWFDRFLNP
Second-generation sequencers (SGS) have been game-changing, achieving cost-effective whole genome sequencing in many non-model organisms. However, a large portion of the genomes still remains unassembled. We reconstructed azuki bean (Vigna angularis) genome using single molecule real-time (SMRT) sequencing technology and achieved the best contiguity and coverage among currently assembled legume crops. The SMRT-based assembly produced 100 times longer contigs with 100 times smaller amount of gaps compared to the SGS-based assemblies. A detailed comparison between the assemblies revealed that the SMRT-based assembly enabled a more comprehensive gene annotation than the SGS-based assemblies where thousands of genes were missing or fragmented. A chromosome-scale assembly was generated based on the high-density genetic map, covering 86% of the azuki bean genome. We demonstrated that SMRT technology, though still needed support of SGS data, achieved a near-complete assembly of a eukaryotic genome.
