Gene_locus Report for: phera-PrKAI2d3

Other strains: Phelipanche ramosa (Hemp broomrape) (Orobanche ramosa) parasitic plant; Phelipanche aegyptiaca

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms: N E > Eukaryota: N E > Viridiplantae: N E > Streptophyta: N E > Streptophytina: N E > Embryophyta: N E > Tracheophyta: N E > Euphyllophyta: N E > Spermatophyta: N E > Magnoliophyta: N E > Mesangiospermae: N E > eudicotyledons: N E > Gunneridae: N E > Pentapetalae: N E > asterids: N E > lamiids: N E > Lamiales: N E > Orobanchaceae: N E > Orobancheae: N E > Phelipanche: N E > Phelipanche ramosa: N E

Title: A Phelipanche ramosa KAI2 protein perceives strigolactones and isothiocyanates enzymatically
de Saint Germain A, Jacobs A, Brun G, Pouvreau JB, Braem L, Cornu D, Clave G, Baudu E, Steinmetz V and Boyer FD <6 more author(s)> de Saint Germain A, Jacobs A, Brun G, Pouvreau JB, Braem L, Cornu D, Clave G, Baudu E, Steinmetz V, Servajean V, Wicke S, Gevaert K, Simier P, Goormachtig S, Delavault P, Boyer FD (- 6)
Ref: Plant Commun, 2:100166, 2021 : PubMed
Abstract


ESTHER: de Saint Germain_2021_Plant.Commun_2_100166
PubMedSearch: de Saint Germain 2021 Plant.Commun 2 100166
PubMedID: 34746757
Gene_locus related to this paper: phera-PrKAI2c, phera-PrKAI2d1, phera-PrKAI2d2, phera-PrKAI2d3, phera-PrKAI2d4

Abstract

Phelipanche ramosa is an obligate root-parasitic weed that threatens major crops in central Europe. In order to germinate, it must perceive various structurally divergent host-exuded signals, including isothiocyanates (ITCs) and strigolactones (SLs). However, the receptors involved are still uncharacterized. Here, we identify five putative SL receptors in P. ramosa and show that PrKAI2d3 is involved in the stimulation of seed germination. We demonstrate the high plasticity of PrKAI2d3, which allows it to interact with different chemicals, including ITCs. The SL perception mechanism of PrKAI2d3 is similar to that of endogenous SLs in non-parasitic plants. We provide evidence that PrKAI2d3 enzymatic activity confers hypersensitivity to SLs. Additionally, we demonstrate that methylbutenolide-OH binds PrKAI2d3 and stimulates P. ramosa germination with bioactivity comparable to that of ITCs. This study demonstrates that P. ramosa has extended its signal perception system during evolution, a fact that should be considered for the development of specific and efficient biocontrol methods.



        

Title: PLANT EVOLUTION. Convergent evolution of strigolactone perception enabled host detection in parasitic plants
Conn CE, Bythell-Douglas R, Neumann D, Yoshida S, Whittington B, Westwood JH, Shirasu K, Bond CS, Dyer KA, Nelson DC
Ref: Science, 349:540, 2015 : PubMed
Abstract


ESTHER: Conn_2015_Science_349_540
PubMedSearch: Conn 2015 Science 349 540
PubMedID: 26228149
Gene_locus related to this paper: phera-PrKAI2c, phera-PrKAI2d1, phera-PrKAI2d2, phera-PrKAI2d3, phera-PrKAI2d4, strhe-ShD14, orocc-OcKAI2d2, trivs-TvKAI2c, strhe-ShKAI2D2, 9lami-a0a2u8xq24, orocc-OcD14, conam-CaKAI2c, 9lami-a0a2u8xq49, 9lami-a0a2u8xq53, oroce-OcKAI2d2, oroce-OcKAI2d3, orocc-OcKAI2d4, orocc-OcKAI2d1, 9lami-a0a2u8xqa1, oroce-OcD14, 9lami-a0a2u8xqb0, strhe-sHKAI2d1, conam-CaD14, 9lami-a0a2u8xqc8, 9lami-a0a2u8xqd2, 9lami-a0a2u8xqe1, 9lami-a0a2u8xqh0, oroce-OcKAI2d1, orocc-OcKAI2c, strhe-ShKAI2c, 9lami-a0a2u8xqv8, orocc-OcKAI2d6, orocc-OcKAI2d5, 9lami-a0a2u8xr73, oroce-OcKAI2c, 9lami-a0a2u8xr89, orocc-OcKAI2d3, 9lami-a0a2u8xrl6, strhe-ShKAI2i, 9lami-a0a2u8xrn7

Abstract

Obligate parasitic plants in the Orobanchaceae germinate after sensing plant hormones, strigolactones, exuded from host roots. In Arabidopsis thaliana, the alpha/beta-hydrolase D14 acts as a strigolactone receptor that controls shoot branching, whereas its ancestral paralog, KAI2, mediates karrikin-specific germination responses. We observed that KAI2, but not D14, is present at higher copy numbers in parasitic species than in nonparasitic relatives. KAI2 paralogs in parasites are distributed into three phylogenetic clades. The fastest-evolving clade, KAI2d, contains the majority of KAI2 paralogs. Homology models predict that the ligand-binding pockets of KAI2d resemble D14. KAI2d transgenes confer strigolactone-specific germination responses to Arabidopsis thaliana. Thus, the KAI2 paralogs D14 and KAI2d underwent convergent evolution of strigolactone recognition, respectively enabling developmental responses to strigolactones in angiosperms and host detection in parasites.